Novel microorganism and its gene fragment order and application

A nucleotide sequence and culture technology, applied in the field of actinomycetes, can solve the problems of antibiotic sensitivity, cure the symptoms but not the root cause, and have no bactericidal effect, and achieve the effect of eliminating breeding and improving the environment.

Inactive Publication Date: 2009-08-19
NORTHWEST A & F UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the strains of microecological preparations are often limited to traditional strains such as bacillus, lactic acid bacteria, bifidobacteria, and yeast. Most of these strains have weak or no bactericidal effect, and most of them are sensitive to commonly used antibiotics. To a certain extent or through indirect methods to prevent and control the occurrence of aquatic animal diseases, but its control effect is limited, and the symptoms are not cured

Method used

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  • Novel microorganism and its gene fragment order and application
  • Novel microorganism and its gene fragment order and application
  • Novel microorganism and its gene fragment order and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1. Isolation and screening of actinomycetes DL2-F-5

[0022] 1) The source of sea mud is collected from the seabed mud layer in Dalian sea area.

[0023] 2), isolation and screening of bacterial strains

[0024] The collected sea mud was airlifted to the laboratory through the sampling box. Take 3g of each sample, add 27mL sterilized deionized water respectively, stir with a magnetic stirrer for 20min, and then 10 -1 Concentration suspension, and then diluted with sterile deionized water to 10 -2 , 10 -3 , 10 -4 , take 0.1mL of the supernatant of each dilution respectively, smear it on the Gaoshi No. 1 medium plate (the medium contains 0.0075% potassium dichromate to inhibit the growth of bacteria and fungi), and put it into a culture medium at 28°C. Cultivate in the box for 7 days, pick colonies of different shapes and cultivate them on the Gaoshi No. 1 slope, and repeatedly streak and purify the isolated colonies on the plate until pure culture is obta...

Embodiment 2

[0026] Example 2. Identification of Actinomycetes DL2-F-5

[0027] 1) Morphological features

[0028] Actinomycetes DL2-F-5 strain was cultured on Gaoshi No. 1 by insert method for 3d, 7d, 15d, and 30d, and observed under an optical microscope. Diaphragm without rupture see ( figure 1 ); less aerial hyphae, spiral or curved, and oval spores ( figure 2 ).

[0029] 2), the characteristics of culture

[0030] Actinomycetes DL2-F-5 strain was cultured on 9 kinds of media at 28°C, and its growth, the color of air filaments and basal filaments and the production of pigments were observed on 3d, 7d, 15d, and 30d respectively. See Table 1.

[0031] Table 1 Culture characteristics of marine actinomycetes DL2-F-5 strain

[0032]

[0033] The components and proportioning of the above-mentioned medium are respectively:

[0034] Sucrose Chapei agar: 30g sucrose, NaNO 3 2g, K 2 HPO 4 1g, KCl 0.5g, MgSO 4 0.5g, FeSO 4 0.01g, 15g agar, 1000mL distilled water, pH 7.2, sterili...

Embodiment 3

[0103] Embodiment 3: Actinomycetes DL2-F-5 aquatic biological preparation using method and dosage

[0104] (1) Technical indicators of actinomycetes DL2-F-5 aquatic biological preparations

[0105] The obtained actinomycetes DL2-F-5 fermentation broth is concentrated 4 times by low temperature (20°C) airflow to make aquatic actinomycetes biological preparations. The preparations have solid and liquid dosage forms, and the solid preparations use bran or humic acid as adsorption Carrier, and determine the relative biological potency ≥ 10mg / g, the number of viable bacteria ≥ 10 9 cfu / g; the liquid preparation is concentrated, the relative biological potency is ≥10mg / mL, and the number of viable bacteria is ≥10 9 cfu / mL. Reach the established standard requirements and then pack in different volumes of packaging bags or bottles.

[0106] (2) Actinomycetes DL2-F-5 aquatic product preparation method and dosage

[0107] The preparation can be added to the feed of aquaculture anima...

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Abstract

A streptomycesfradiae DL2-F-5 is separated from marine or fresh water microbe beneficial bacterial group. It can produce antibacterial substances or increase immune active ingredients, prevent aquatic animal diseases, avoid destruction for purified water environment by antiseptics, inhibit pathogenic bacteria multiplying, decompose organic pollutant and residual baits in water body and improve water-body environment. It can be used in medicine, food and feed additives production.

Description

technical field [0001] The present invention relates to a novel actinomycete isolated from marine or fresh water microbial beneficial flora, as well as the gene sequence and application of the 16SrRNA of the actinomycete. technical background [0002] In recent years, my country's aquaculture industry has gradually transitioned from extensive aquaculture to intensive and large-scale aquaculture, which has not only increased the variety of aquaculture, but also greatly increased production and enriched people's lives. However, due to the continuous expansion of the scale of intensive farming, the serious pollution of the breeding water, and the destruction of the ecological environment of the breeding, the diseases of aquatic animals are becoming more and more rampant, which seriously restricts the development of the aquaculture industry. [0003] For a long time, in order to prevent the occurrence of diseases, chemical drugs have been widely used in my country as a fast, eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N15/31C02F3/34A61K35/74A23K1/16A61P31/00C12R1/54A23K10/18A23K20/10
Inventor 王高学顾忠旗叶道林原居林穆杨黄海洪付维法梁朝军崔婧姚嘉赟
Owner NORTHWEST A & F UNIV
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