Streptomyces sp. and screening method and application thereof
A screening method and technology of Streptomyces, applied in the field of microbiology, can solve the problems of wide host range, destructive long-term, no chemical control methods, etc., and achieve the effect of significant antagonism
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Embodiment 1
[0028] Example 1. Screening and identification of Streptomyces sp. NEAU-HV9.
[0029] 1. Isolation and cultivation of strains
[0030] The soil samples were collected in Bama Yao Autonomous County, Hechi City, Guangxi Zhuang Autonomous Region, China (24°15′N,107°26′E). 5g of the dried soil samples were dissolved in a 45ml Erlenmeyer bottle of sterile water (with glass beads) , Shake at 180rpm for 30-45 minutes, after standing for 10 minutes, take the upper layer liquid and dilute it by 1000 times, then pipet 200μl of the diluted liquid on HV medium (humic acid 0.1g, MgSO 4 ·7H 2 O 0.05g, KCl 0.17g, Na 2 HPO 4 ·3H 2 O 0.05g, FeSO 4 0.001g, CaCO 3 0.002g, multivitamin 0.1ml, agar 2g, sterile water 100ml) plate medium, placed at 28℃ for 7 days, pick a single colony to inoculate fresh ISP3 plate medium (Oats 2g, K 2 HPO 4 ·3H 2 O 0.05g, MgSO 4 ·7H 2 O 0.02g, KNO 3 0.02g, agar 2g, sterile water 100ml), the purified strain was obtained by repeated transfer, and the purified strain was n...
Embodiment 2
[0046] Example 2. Determination of the antagonistic activity of Streptomyces sp. NEAU-HV9.
[0047] Plant pathogen species and source: Tomato bacterial wilt pathogen Pseudomonas solanacearum is a well-known pathogen in the field that causes tomato bacterial wilt. The strain used in the present invention is provided by the State Key Laboratory of Plant Diseases and Insect Pest Biology, Institute of Plant Protection, Chinese Academy of Agricultural Sciences.
[0048] Preparation of tomato bacterial wilt pathogen suspension: 100 mL of physiological saline (0.9%) was used to wash the bacterial wilt pathogen in the eggplant bottle out of the conical flask for use.
[0049] Antagonistic activity determination: using agar well diffusion method. The specific steps are as follows: inoculate the strains in ISP2 liquid medium, culture in a shake flask to logarithmic phase at 28°C and 250 rpm, and inoculate the seed liquid in the fermentation medium (maltodextrin 4g, Lactose 4g, yeast extract p...
Embodiment 3
[0052] Example 3. Determination of the biological control characteristics of Streptomyces sp.NEAU-HV9 against tomato bacterial wilt at the tomato seedling stage.
[0053] Fill a sterile container with about 15-20ml of bacterial wilt pathogen suspension (OD 600 =0.3), take the sprouted seedlings, set up a total of 3 treatments, the first group, the positive control, the roots of the seedlings are immersed in sterile water for about 30s, then the seedlings are transferred to an empty 1.5 or 2 ml sterile EP tube ; The second group, the negative control, immersed the roots of the seedlings in the bacterial suspension (until the root-stem junction) for about 30 seconds, and then transferred the seedlings to an empty 1.5 or 2 ml sterile EP tube; the third group, In the treatment group, the roots of the seedlings were immersed in the bacterial suspension (until the root-stem junction) for about 30 seconds, and then the roots of the seedlings were respectively immersed in the antagonistic...
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