Method for promoting growth of rice and promoting rice to absorb iron element in iron deficiency environment

An iron deficiency and iron technology, applied in the field of maintaining the growth of rice, can solve the problems of insufficient iron intake, reduced chlorophyll synthesis, decreased biomass, etc., and achieves convenient, simple and diversified processing, promotes growth of iron absorption, and promotes iron. The effect of elemental absorption

Active Publication Date: 2020-07-31
湖南新长山农业发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the total content of iron in the soil is very rich, the effective content of iron in the soil is very low, especially in neutral and calcareous soils, most of which exist in the form of trivalent cations, with extremely low sol...

Method used

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  • Method for promoting growth of rice and promoting rice to absorb iron element in iron deficiency environment
  • Method for promoting growth of rice and promoting rice to absorb iron element in iron deficiency environment
  • Method for promoting growth of rice and promoting rice to absorb iron element in iron deficiency environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Under normal circumstances, foliar spraying Streptomyces sp. OsiSh-2, promoting rice growth

[0030] Experimental site: soil culture in greenhouse

[0031] Experimental crop: Rice (Indica 93-11).

[0032] Experimental treatment group: preparation Streptomyces sp. OsiSh-2 spore suspension at a concentration of 1 × 10 8 CFUmL -1 , adding 0.2% Tween 20 for treatment; water containing 0.2% Tween 20 was used as a control. The specific method for preparing the spore suspension is as follows:

[0033] (1) Inoculate the strain on ISP-2 solid medium and culture it statically in a constant temperature incubator at 30 °C for 14 days.

[0034] (2) Use an inoculation loop to collect the mature bacteria in step 1), transfer them to a conical flask containing sterile distilled water and glass beads, shake vigorously and then filter to obtain a spore suspension.

[0035] (3) Count the spore suspension obtained in step (2) with a hemocytometer to adjust its concentr...

Embodiment 2

[0041] Example 2: Under normal circumstances, Streptomyces sp. OsiSh-2 entraps rice seeds and promotes rice growth

[0042] Experimental conditions: Rice is grown hydroponically in a greenhouse constant temperature light incubator, the photoperiod is 16 hours of light / 8 hours of darkness, the cultivation temperature is 28-30°C, and the air humidity is 60-70%

[0043] Experimental crop: Rice (Indica 93-11).

[0044] Experimental treatment group: prepared according to the method of Example 1 Streptomyces sp. OsiSh-2 spore suspension at a concentration of 1 × 10 8 CFU mL -1 , the ratio of rice seeds / 0.3% (v / v) xanthan gum / spore suspension was 1g / 463 μl / 51 μl to embed the sterilized rice seeds, and rice seeds / 0.3% (v / v) The ratio of xanthan gum / sterile water is 1 g / 463 μl / 51 μl, as a control. Each treatment was repeated three times, and each group contained 48 rice seedlings. Three weeks after germination, the growth of rice was observed and its agronomic traits were ...

Embodiment 3

[0048] Embodiment 3: under iron deficiency situation, Streptomyces sp. OsiSh-2 entraps rice seeds to promote rice growth and iron absorption

[0049] Experimental conditions: Rice is grown hydroponically in a greenhouse constant temperature light incubator, the photoperiod is 16 hours of light / 8 hours of darkness, the cultivation temperature is 28-30°C, and the air humidity is 60-70%

[0050] Experimental crop: Rice (Indica 93-11).

[0051] Experimental treatment group: prepared according to the method of Example 1 Streptomyces sp. OsiSh-2 spore suspension at a concentration of 1 × 10 8 CFU mL -1 , the ratio of rice seeds / 0.3% (v / v) xanthan gum / spore suspension was 1g / 463 μl / 51 μl to embed the sterilized rice seeds, and rice seeds / 0.3% (v / v) The ratio of xanthan gum / sterile water was 1g / 463 μl / 51 μl, as a control, each treatment was repeated three times, and each group contained 48 rice seedlings. After germination, it was planted for one week under normal conditio...

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Abstract

The invention discloses a method capable of promoting growth of rice in a normal growth environment and capable of promoting the rice to absorb an iron element in an environment lacking of directly available iron. According to the method, a strain of Streptomycessp. OsiSh-2 is utilized, and a spore suspension thereof is employed; the rice under the normal condition can be treated with two different measures: (1) the spore suspension is sprayed on leaf surfaces of the rice, and (2) disinfected rice seeds are embedded with the spore suspension of Streptomycessp. OsiSh-2. The method disclosed bythe invention promotes the growth of the rice, and increases the chlorophyll content and the photosynthesis rate, and thus, rice biomass including plant height, root length, the fresh and dry weight of aboveground and underground parts and the like of the rice is significantly increased; and under the adverse situation of iron deficiency, the disinfected rice seeds are embedded with the spore suspension of Streptomycessp. OsiSh-2, so that the iron content in the rice is significantly increased, the normal growth of the rice under iron deficiency stress is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of biofertilizers, and specifically relates to using a rice endophytic actinomycete to promote the growth of rice under normal growth conditions and to promote the absorption of iron by rice in an environment lacking direct use of iron, so as to maintain the growth of rice method. Background technique [0002] Rice is an important food crop in my country and the world, and its yield and quality are closely related to human health. The means to promote rice growth currently mainly include spraying chemical or biological fertilizers. With the formulation of the zero-growth strategy of chemical fertilizers in my country, more and more attention has been paid to the research on environmentally friendly biological fertilizers. For example, the photosynthetic bacteria Rhodopseudomonas palustris PSB06 inoculum reported by Zeng Yibo et al. can promote the growth of rice seedlings; Trichoderma spp.'s biofertilizer ...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01C1/06A01G7/06C12N1/20C12R1/465
CPCA01G22/22A01C1/06A01G7/06C12N1/20
Inventor 朱咏华高艳曹力丹刘选明喻金兰卜志刚姚晴晴
Owner 湖南新长山农业发展股份有限公司
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