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Preparation and application of a composite microbial agent for coal gangue artificial ecological matrix

A compound microbial agent and artificial ecology technology, applied in the field of environmental biology, can solve the problems of small number and types of active microorganisms, difficulty in using nutrients directly by plants, increasing the number of active microorganisms in the artificial matrix of coal gangue, etc., to achieve the improvement of the types of active microorganisms and quantity, improving soil environmental quality, and improving the physical and chemical properties of the matrix

Active Publication Date: 2022-06-03
北京优生基生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: to provide a compound microbial functional bacterial agent for coal gangue artificial ecological matrix, to solve the problems that the nutrients in coal gangue are difficult to be directly utilized by plants, and the types and quantities of active microorganisms are small, and can be used in coal gangue. Prepare the artificial ecological matrix to make it mature, in which the refractory nutrients are effectively released, increase the number of active microorganisms in the artificial matrix of coal gangue, and realize the resource utilization effect of coal gangue

Method used

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  • Preparation and application of a composite microbial agent for coal gangue artificial ecological matrix
  • Preparation and application of a composite microbial agent for coal gangue artificial ecological matrix
  • Preparation and application of a composite microbial agent for coal gangue artificial ecological matrix

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Example 1 Study on Antagonism Between Strains

[0021]The above-mentioned 5 strains of actinomycetes were streaked with Pseudomonas on the Gao's No. 1 and LB solid medium containing equal volumes, respectively, and the 5 strains of actinomycetes were streaked with Penicillium oxalicum on the solid medium containing equal volumes. On the Gao's No. 1 and PDA solid medium, Pseudomonas rod and Penicillium oxalicum were streaked on the solid medium containing equal volumes of LB and PDA. . If a sterile area is formed at the intersection of the two bacteria, it means that there is a bacteriostatic zone between the two bacteria, and the two bacteria are antagonistic to each other and cannot be combined together. combination. From the results, there is no antagonism between the various strains, and they will not interfere with each other and affect the growth of the strains, so they can be mixed to make a compound bacterial agent.

[0022] Table 1 The results of the strain a...

Embodiment 2

[0026] Example 2 Effects of different strain combinations on physical and chemical indexes and microorganisms of coal gangue artificial ecological matrix

[0027] In the functional microbial inoculum of the present invention, 5 kinds of actinomycetes (including Streptomyces violaceum, Streptomyces thermoamylase, Streptomyces thermophilus carbon monoxide, Streptomyces lepidopianus, Streptomyces vulgaris), 1 species of bacteria (Pseudomonas vulgaris monas), 1 mold (Penicillium oxalicum). Five strains of Streptomyces were inoculated into sterile ISP2 liquid medium, pH 7.2-7.4, cultured at 50°C, 120rpm shaker conditions, and one strain of Pseudomonas was inoculated into sterile LB liquid medium, pH 7.2- 7.4 Incubate for 12h under 30℃, 150rpm shaker conditions, inoculate one strain of Penicillium oxalicum into potato liquid medium with natural pH value, and cultivate under 28℃, 170rpm shaker conditions for 96h. Set different strain combinations: treatment 1, 5 kinds of actinomycet...

Embodiment 3

[0030] The preparation of embodiment 3 compound bacterial agent

[0031] (1) Bacterial activation: take the slant of the microorganism of the present invention stored at 4°C, inoculate 5 kinds of actinomycetes into Gao's No. 1 solid plate medium respectively, cultivate in a constant chamber at 50°C for 3 days, and inoculate 1 kind of bacteria into LB solid plate The culture medium was cultured in a constant chamber at 35°C for 2 days, and one mold was inoculated into the PDA solid medium and cultured in a constant chamber at 28°C for 4 days to achieve bacterial activation.

[0032] (2) Seed liquid preparation: transfer the 5 kinds of actinomycetes to 200mL sterile ISP2 liquid culture medium, pH 7.2-7.4, 50°C, 120rpm shaker, respectively, on the strain plate activated by the inclined plane in step (1). Cultured under conditions, 1 type of bacteria was transferred to 200mL sterile LB liquid medium, pH 7.2-7.4, cultivated under the conditions of 35 ° C, 150rpm shaker, 1 type of m...

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Abstract

The invention discloses a compound microbial bacterial agent used for coal gangue artificial ecological matrix, including 5 kinds of actinomycetes: Streptomyces violaceum ( Streptomyces thermoviolaceus ), Streptomyces thermophilus ( S.thermodiastaticus ), Streptomyces carbon monoxide ( S.thermocarboxydus ), Streptomyces albus ( S. albidoflavus ) and Streptomyces vulgaris ( S. thermovulgaris ), 1 bacteria: Pseudomonas ( Pseudomonas sp.), and 1 mold: Penicillium oxalicum ( Penicillium oxalicum ). The preparation process of the bacterial agent includes four steps: activation of the bacterial strain, preparation of the seed liquid, preparation of the liquid composite bacterial agent, and preparation of the solid bacterial agent. The technology of the invention solves the problem that the coal gangue lacks microorganisms and the nutrients are not conducive to plant absorption and utilization, and provides a new way for the resource utilization of the coal gangue and landscaping waste.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a preparation, preparation and application of a composite microbial inoculant for a coal gangue artificial ecological substrate. Background technique [0002] Coal gangue is an associated product in coal production, and its output is 10%-20% of coal production. It is one of the largest industrial solid wastes in my country. The natural stacking of coal gangue not only occupies a large amount of land, but also causes irreversible pollution to the surrounding environment and groundwater. The main treatment methods of coal gangue include landfill, coal gangue power generation, industrial extraction of certain substances, and brick and cement production in the construction industry. In recent years, the use of coal gangue as a component of soil amendments or the preparation of compound fertilizers has also begun to be reported, but the general dosage is rel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C05G3/80A01G24/20A01G24/10C12R1/465C12R1/38C12R1/80
CPCC12N1/20C12N1/14C05G3/00A01G24/20A01G24/10Y02W30/40
Inventor 彭霞薇代兴和乔浩亮周金星
Owner 北京优生基生态科技有限公司
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