Preparation and application of protein hydrolyzate of chilled Antarctic krill

A technology of protein hydrolyzate and Antarctic krill, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems that there are no literature reports on the use of Antarctic krill protein, and achieve good development prospects and raw materials The effect of rich source and high protein content

Active Publication Date: 2016-09-28
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no literature report on the method of using alkaline protease to hydrolyze Antarctic krill protein, and there is no literature report on the use of Antarctic krill protein and its hydrolyzate as raw materials for microbial fermentation

Method used

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  • Preparation and application of protein hydrolyzate of chilled Antarctic krill
  • Preparation and application of protein hydrolyzate of chilled Antarctic krill
  • Preparation and application of protein hydrolyzate of chilled Antarctic krill

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 1.0kg of chilled Antarctic krill raw material, add it to 1.2L of water at a mass-volume ratio of 1.2 times, grind and homogenize at a speed of 250rpm / min for 5min, add alkaline protease (enzyme activity 1400U / mL) 10mL, and enzymolyze at 40°C for 2h; Filter under reduced pressure with a medium-speed qualitative filter paper in a Buchner funnel, take the aqueous solution and centrifuge at 4°C, 9000 rpm for 30 minutes, concentrate and dry the supernatant to obtain a protein hydrolyzate, 12.6 g, with a yield of 1.26%. The obtained Antarctic krill protein hydrolyzate contains 0% fat and 63% peptides or amino acids in total.

Embodiment 2

[0034] Take 1.0kg of chilled Antarctic krill raw material, add it to 1.5L of water at a mass-volume ratio of 1.5 times, grind and homogenize at a speed of 250rpm / min for 5min, add 7mL of alkaline protease (enzyme activity 2000U / mL) and enzymolyze at 45°C for 2h; Filtrate under reduced pressure with a medium-speed qualitative filter paper in a Buchner funnel, take the aqueous solution and centrifuge at 4 °C, 9000 rpm for 30 min, and concentrate and dry the supernatant to obtain a protein hydrolyzate, 13.7 g of the product, with a yield of 1.37%. The obtained Antarctic krill protein hydrolyzate contains 0% fat and 61% peptides or amino acids in total.

Embodiment 3

[0036] Take 1.0kg of chilled Antarctic krill raw material, add it into 3L of water according to the mass-volume ratio of 3 times, grind and homogenize it at a speed of 250rpm / min for 5min, add 14mL of alkaline protease (enzyme activity 2000U / mL) and enzymolyze it at 50℃ for 3h; The Buchner funnel was filtered with qualitative filter paper under reduced pressure, and the aqueous solution was centrifuged at 4°C and 9000 rpm for 30 minutes. The supernatant was concentrated and dried to obtain a protein hydrolyzate, 18.4 g, with a yield of 1.84%. The obtained Antarctic krill protein hydrolyzate contains 0% fat and 71% peptides or amino acids in total.

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Abstract

The invention relates to a method of preparing protein hydrolysate of chilled Antarctic krill. The method comprises the following steps: (1) mixing chilled Antarctic krill raw material with water in a ratio of 1-3L water per kilogram raw material, grinding and homogenizing, adding alkaline protease, and performing enzymolysis for 1-5 hours at the temperature of 35-55 DEG C; (2) performing solid-liquid separation to obtain clear liquor, concentrating and drying the clear liquor to obtain the protein hydrolysate, wherein the content of fat is 0%, and the total content of peptides and amino acid is more than 60%. The total yield of actinomycin produced through fermentation by virtue of streptomycete in an Antarctic krill protein hydrolysate group is equivalent to the yield of the existing commercialization nitrogen source group, and high performance liquid chromatography-mass spectrum analysis results show that the diversity of analogue of actinomycin D produced through fermentation in the Antarctic krill protein hydrolysate group is better.

Description

technical field [0001] The invention belongs to the field of preparation of enzymatic hydrolyzate of Antarctic krill protein, in particular to a preparation method of protein hydrolyzate of ice-fresh Antarctic krill and its use as a microbial fermentation raw material. Background technique [0002] Antarctic krill, also known as Antarctic krill, Latin name Euphausia superba Dana, crustaceans, is the largest marine plankton in the waters near Antarctica, with a biomass of 650-1000 million tons. Antarctic krill is rich in active ingredients such as protein and lipids, especially the crude protein content in dried Antarctic krill muscle is as high as 64%, and the total amount of 18 kinds of amino acids in krill protein is 57%, and the content of 8 kinds of essential amino acids is higher than 25%, accounting for more than 43% of the total amino acids. Therefore, the preparation of krill protein or its hydrolyzate from resource-rich Antarctic krill has broad application prospec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C12P21/02C12R1/465
Inventor 樊成奇黄艳青杨桥田晓清王成陆亚男马丽艳黄洪亮
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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