Preparation and application of protein hydrolyzate of chilled Antarctic krill
A technology of protein hydrolyzate and Antarctic krill, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems that there are no literature reports on the use of Antarctic krill protein, and achieve good development prospects and raw materials The effect of rich source and high protein content
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Embodiment 1
[0032] Take 1.0kg of chilled Antarctic krill raw material, add it to 1.2L of water at a mass-volume ratio of 1.2 times, grind and homogenize at a speed of 250rpm / min for 5min, add alkaline protease (enzyme activity 1400U / mL) 10mL, and enzymolyze at 40°C for 2h; Filter under reduced pressure with a medium-speed qualitative filter paper in a Buchner funnel, take the aqueous solution and centrifuge at 4°C, 9000 rpm for 30 minutes, concentrate and dry the supernatant to obtain a protein hydrolyzate, 12.6 g, with a yield of 1.26%. The obtained Antarctic krill protein hydrolyzate contains 0% fat and 63% peptides or amino acids in total.
Embodiment 2
[0034] Take 1.0kg of chilled Antarctic krill raw material, add it to 1.5L of water at a mass-volume ratio of 1.5 times, grind and homogenize at a speed of 250rpm / min for 5min, add 7mL of alkaline protease (enzyme activity 2000U / mL) and enzymolyze at 45°C for 2h; Filtrate under reduced pressure with a medium-speed qualitative filter paper in a Buchner funnel, take the aqueous solution and centrifuge at 4 °C, 9000 rpm for 30 min, and concentrate and dry the supernatant to obtain a protein hydrolyzate, 13.7 g of the product, with a yield of 1.37%. The obtained Antarctic krill protein hydrolyzate contains 0% fat and 61% peptides or amino acids in total.
Embodiment 3
[0036] Take 1.0kg of chilled Antarctic krill raw material, add it into 3L of water according to the mass-volume ratio of 3 times, grind and homogenize it at a speed of 250rpm / min for 5min, add 14mL of alkaline protease (enzyme activity 2000U / mL) and enzymolyze it at 50℃ for 3h; The Buchner funnel was filtered with qualitative filter paper under reduced pressure, and the aqueous solution was centrifuged at 4°C and 9000 rpm for 30 minutes. The supernatant was concentrated and dried to obtain a protein hydrolyzate, 18.4 g, with a yield of 1.84%. The obtained Antarctic krill protein hydrolyzate contains 0% fat and 71% peptides or amino acids in total.
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