Quantitative detection method for saponin in tea saponin

A quantitative detection method and technology for tea saponin, applied in the field of detection, can solve the problems of high viscosity, low content and dark black color of tea saponin, and achieve the effects of high repeatability and accuracy, simple equipment and easy operation.

Inactive Publication Date: 2012-08-01
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The quantitative detection methods of saponins in tea saponin reported in the past mainly include: gravimetric analysis, vanillin-concentrated sulfuric acid colorimetry, fluorescence photometry, velocity difference Spectrophotometry, thin layer scanning method, HPLC method and liquid chromatography-time-of-flight mass spectrometry (LC/TOF-MS) coupled instrument method, etc., but no special research has been done on the scope and accuracy of these methods
Extracting tea saponin from Camellia oleifera cake, there have been many research reports and patented technology announcements in history, such as: "A new process for the integration of solvent extraction and selective separation to prepare tea saponin" (public invention patent number: 200710168737.4 , 2007, Yan Liushui, Luo Xubiao, Tai Jiandong, Tu Xinman, Yu Bin, 2007), "A Method for Extracting Tea Saponin" (authorized invention patent number: ZL99115525.4, Fang Xiamin, 2008), but r...

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  • Quantitative detection method for saponin in tea saponin
  • Quantitative detection method for saponin in tea saponin
  • Quantitative detection method for saponin in tea saponin

Examples

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Embodiment 1

[0039] Embodiment 1: Gravimetric method, vanillin-concentrated sulfuric acid method and HPLC quantitative detection of camellia oleifera seed meal saponins

[0040] (1) Quantitative detection by gravimetric method

[0041]Prepare the following reagents: (1) Methanol (analytical grade), hydrochloric acid (ρ=1.18 g / mL), sodium hydroxide (analytical grade), acetone (analytical grade), 20% hydrochloric acid solution (measure 504 mL of hydrochloric acid, add water Dilute to 1000 mL), sodium hydroxide methanol solution (weigh 10 g of sodium hydroxide, dissolve in 20 mL of water, add 80 mL of methanol) and mix well. Weigh 3.0~3.5g (accurate to 0.0001g) of powdered Camellia oleifera seed meal sample, put it in an Erlenmeyer flask, add 25 mL of sodium hydroxide methanol solution, connect it with a serpentine condenser, and place it in a fume hood for reflux in a boiling water bath for 2 h , take out and cool to room temperature. Adjust the above solution to pH 7 with 20% hydrochl...

Embodiment 2

[0055] Example 2: Quantitative detection of high-purity tea saponin and tea saponin crude extract by gravimetric method, vanillin-concentrated sulfuric acid method and HPLC method

[0056] (1) Quantitative detection by gravimetric method

[0057] Prepare the following reagents: (1) Methanol (analytical grade), hydrochloric acid (ρ=1.18 g / mL), sodium hydroxide (analytical grade), acetone (analytical grade), 20% hydrochloric acid solution (measure 504 mL of hydrochloric acid, add water Dilute to 1000 mL), sodium hydroxide methanol solution (weigh 10 g of sodium hydroxide, dissolve in 20 mL of water, add 80 mL of methanol) and mix well. Weigh 3.0-3.5 g of high-purity tea saponin and tea saponin crude extract sample, put it in an Erlenmeyer flask, add 25 mL of sodium hydroxide methanol solution, connect a serpentine condenser, and place it in a fume hood for reflux in a boiling water bath 2 h, removed and cooled to room temperature. The above solution was adjusted to pH 7 wi...

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Abstract

The invention discloses a quantitative detection method for saponin in tea saponin. According to the quantitative detection method, 3.0g-3.5g of extract of tea seed or the tea saponin is taken, the extract is subjected to alkali and acid hydrolysis and then is dissolved in water, hydrolysate is poured into the water to allow precipitation of the extract so as to measure mass of sapogenin, and then the mass of the sapogenin is converted to mass fraction of the saponin. The quantitative detection method has the characteristics that equipment is simple, the cost is low, the operation is convenient and easy, the accuracy is high, and the direct detection can be preformed, thus being suitable for detection on tea seeds, crude extract of the tea saponin, and high-purity tea saponin. The quantitative detection method is particularly applicable to pioneering small and medium-sized enterprises which are lack of instruments and equipment and are equipped with personnel with low education degree.

Description

technical field [0001] The invention relates to a detection technology, in particular to a quantitative detection method for saponins in tea saponin. Background technique [0002] Tea saponin, also known as tea saponin and tea saponin, is a pentacyclic triterpenoid saponin composed of seven ligands, four glycosides and two organic carboxylic acids, mainly derived from the camellia plants (such as tea, Camellia, Camellia). Tea saponin has the properties of antibacterial, anti-oxidation, anti-high cholesterol and high blood fat, moist foaming, etc. It is widely used in industries such as medicine, building materials, aquatic products, pesticides, cosmetics, and detergents. It is used as a green and environmentally friendly nonionic surfactant , has a good application prospect. my country is the country with the largest output of Camellia oleifera in the world. At present, the area of ​​Camellia oleifera forest has reached 3.6 million hectares, accounting for more than 80% of...

Claims

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Application Information

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IPC IPC(8): G01N5/02
Inventor 沈立荣陈莹刘松柏祝洪刚蒋炎阳
Owner ZHEJIANG UNIV
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