Quantitative detection method for saponin in tea saponin

Abstract

The invention discloses a quantitative detection method for saponin in tea saponin. According to the quantitative detection method, 3.0g-3.5g of extract of tea seed or the tea saponin is taken, the extract is subjected to alkali and acid hydrolysis and then is dissolved in water, hydrolysate is poured into the water to allow precipitation of the extract so as to measure mass of sapogenin, and then the mass of the sapogenin is converted to mass fraction of the saponin. The quantitative detection method has the characteristics that equipment is simple, the cost is low, the operation is convenient and easy, the accuracy is high, and the direct detection can be preformed, thus being suitable for detection on tea seeds, crude extract of the tea saponin, and high-purity tea saponin. The quantitative detection method is particularly applicable to pioneering small and medium-sized enterprises which are lack of instruments and equipment and are equipped with personnel with low education degree.

Description

technical field [0001] The invention relates to a detection technology, in particular to a quantitative detection method for saponins in tea saponin. Background technique [0002] Tea saponin, also known as tea saponin and tea saponin, is a pentacyclic triterpenoid saponin composed of seven ligands, four glycosides and two organic carboxylic acids, mainly derived from the camellia plants (such as tea, Camellia, Camellia). Tea saponin has the properties of antibacterial, anti-oxidation, anti-high cholesterol and high blood fat, moist foaming, etc. It is widely used in industries such as medicine, building materials, aquatic products, pesticides, cosmetics, and detergents. It is used as a green and environmentally friendly nonionic surfactant , has a good application prospect. my country is the country with the largest output of Camellia oleifera in the world. At present, the area of ​​Camellia oleifera forest has reached 3.6 million hectares, accounting for more than 80% of...

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Problems solved by technology

[0004] The quantitative detection methods of saponins in tea saponin reported in the past mainly include: gravimetric analysis, vanillin-concentrated sulfuric acid colorimetry, fluorescence photometry, velocity difference Spectrophotometry, thin layer scanning method, HPLC method and liquid chromatography-time-of-flight mass spectrometry (LC / TOF-MS) coupled instrument method, etc., but no special research has been done on the scope and accuracy of these methods

Extracting tea saponin from Camellia oleifera cake, there have been many research reports and patented technology announcements in history, such as: "A new process for the integration of solvent extraction and selective separation to prepare tea saponin" (public invention patent number: 200710168737.4 , 2007, Yan Liushui, Luo Xubiao, Tai Jiandong, Tu Xinman, Yu Bin, 2007), "A Method for Extracting Tea Saponin" (authorized invention patent number: ZL99115525.4, Fang Xiamin, 2008), but related patents generally only It involves extraction, refining and utilization, but there is no detection method suitable for camellia oleifera seed meal, crude tea saponin extract and high-purity tea saponin

In recent years, the process of extracting residual oil from tea dregs with n-butanol has been very mature, and has been scaled up in many oil and fat enterprises, but there is no report on the quantitative determination of tea saponin

At the same time, when tea saponin is extracted from camellia oleifera seed dregs, due to the presence of many impurities such as oil, resin, protein, polysaccharides, and tannins, the tea saponin product has high viscosity, low content, and deep black color. The content of tea saponin varies with varieties, cultivation environments and quantitative methods, which causes many difficulties for the quantitative analysis of tea saponin. Therefore, there is no unified mature detection method at home and abroad so far.

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