Method capable of promoting Trichosanthes kirilowii tissue culture seedling differentiation
A technology of melon and culture medium is applied in the field of promoting the differentiation of cultivated seedlings of melon, which can solve the problems of long time for seed propagation, uncoordinated ratio of male and female plants, slow root propagation and germination, etc., so as to accelerate wound healing, increase The effect of using many ways and results
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Embodiment 1
[0020] A method for promoting the differentiation of Trichosanthes group cultured seedlings, comprising the following steps:
[0021] (1) Selection and treatment of explants: Select excellent plants with vigorous growth, more fruits, and strong stress resistance, and cut young leaves from the petiole, which will not cause damage to the Trichosanthes plant, and will not affect the growth and development of Trichosanthes. As a result, tender leaves come from a wide variety of sources, and tissue culture can be carried out at any stage, and the planting efficiency is significantly improved. They are directly placed in 4°C and 2.1% saline solution for 25 minutes, and then placed at 22°C after taking them out. Soak in 3% acetic acid solution for 40 minutes, rinse with running water for 20 minutes, soak in nutrient solution for 2 hours, and then rinse with sterile water for 3 to 4 times. The nutrition is balanced, the absorption is promoted, and the reproduction of pathogenic microor...
Embodiment 2
[0033] A method for promoting the differentiation of Trichosanthes group cultured seedlings, comprising the following steps:
[0034] (1) Selection and treatment of explants: Select excellent plants with vigorous growth, more fruits, and strong stress resistance, and cut young leaves from the petiole, which will not cause damage to the Trichosanthes plant, and will not affect the growth and development of Trichosanthes. As a result, tender leaves come from a wide range of sources, and tissue culture can be carried out at any stage, and the planting efficiency is significantly improved. They are directly placed in 5°C and 2.2% saline solution for 28 minutes, and then placed at 23°C after taking them out. Soak in 3% acetic acid solution for 40 minutes, rinse with running water for 20 minutes, soak in nutrient solution for 2.5 hours, and then rinse with sterile water for 3 to 4 times. The nutrition is balanced, promotes absorption, inhibits the reproduction of pathogenic microorga...
Embodiment 3
[0046] A method for promoting the differentiation of Trichosanthes group cultured seedlings, comprising the following steps:
[0047] (1) Selection and treatment of explants: Select excellent plants with vigorous growth, more fruits, and strong stress resistance, and cut young leaves from the petiole, which will not cause damage to the Trichosanthes plant, and will not affect the growth and development of Trichosanthes. As a result, the source of young leaves is wide, and tissue culture can be carried out at any period, and the planting efficiency is significantly improved. They are directly placed in 6°C and 2.3% saline solution for 30 minutes, and then placed at 24°C after taking them out. Soak in 4% acetic acid solution for 45 minutes, rinse with running water for 20 minutes, soak in nutrient solution for 3 hours, and then rinse with sterile water for 3 to 4 times. The nutrition is balanced, promotes absorption, inhibits the reproduction of pathogenic microorganisms, and can...
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