Nanofiber substrate as well as preparation method of nanofiber substrate and applications of nanofiber substrate

A nanofiber and substrate technology, applied in the field of biomedical materials, can solve the problems of CTC purity and low biological activity

Inactive Publication Date: 2018-01-30
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a nanofiber substrate and its preparation method, as well as the application of the nanofiber substrate in CTC capture and release, so as to solve the problems of low purity and biological activity of CTC capture

Method used

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  • Nanofiber substrate as well as preparation method of nanofiber substrate and applications of nanofiber substrate
  • Nanofiber substrate as well as preparation method of nanofiber substrate and applications of nanofiber substrate
  • Nanofiber substrate as well as preparation method of nanofiber substrate and applications of nanofiber substrate

Examples

Experimental program
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Embodiment 1

[0072] Step 1: Prepare a glass substrate.

[0073] Step 2: Depositing a nanostructure layer on a glass substrate. Specifically: build an electrospinning device, which includes a high-voltage power supply, a micro-injection pump, a 5mL disposable syringe, and a receiving station; transfer the electrospinning solution prepared in the preparation example to a 5mL disposable syringe, and the syringe Use 5-7# needles for the needle, adjust the distance between the receiving station and the needle of the syringe to 10-18cm, adjust the voltage of the high-voltage power supply to 15-24kV, place the glass substrate on the receiving station, connect the high-voltage power supply, The injector is jet-spun to the receiving station, and the time of controlling the injector jet-spinning is 2min, so that a nanostructure layer with a thickness of no more than 200-300nm is produced, and the nanostructure layer is connected on the glass substrate, and the nanostructure layer is made of Chitosa...

Embodiment 2

[0082] The difference between this embodiment and Embodiment 1 is that in step 2 of this embodiment, the injection spinning time of the injector is controlled to be not less than 8 minutes, and a nanostructure layer with a thickness of not less than 5-10 μm is obtained, so that the nanostructure layer attached to a glass substrate. Other preparation steps are the same as in Example 1, and the nanofiber substrate prepared in this example is named JD-2. Such as figure 1 a) and figure 1 Shown in b), be the macrophotograph and the SEM image of the chitosan nanofibers that make in the present embodiment, wherein, figure 1 a) From right to left, respectively, the macrophotograph, the SEM image at 10 μm and the SEM image at 1 μm of the chitosan nanofibers prepared in this example.

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Abstract

The invention relates to a nanofiber substrate as well as a preparation method of the nanofiber substrate and applications of the nanofiber substrate in CTC efficient capture and non-destructive release. The nanofiber substrate provided by the invention comprises a lining substrate, a nano-structure layer, an adhesion-resisting molecular layer and an affinity capture molecular layer which are connected in sequence. The preparation method of the nanofiber substrate comprises the following steps: preparing for the lining substrate; depositing the nano-structure layer on the lining substrate; grafting the adhesion-resisting molecular layer to the nano-structure layer; and connecting the affinity capture molecular layer on the adhesion-resisting molecular layer. The nanofiber substrate provided by the invention has good cell compatibility, the captured CTC sample has relative high activity and purity, and free CTC samples can be obtained through the hybridization effect of complementary chains and aptamers.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a nanofiber substrate, a preparation method thereof, and an application of the nanofiber substrate in CTC capture and release. Background technique [0002] CTC (circulating tumor cell) is the abbreviation of circulating tumor cells. Circulating tumor cells are a general term for all kinds of tumor cells that exist in peripheral blood. Most of the CTCs undergo apoptosis or are phagocytized after entering the peripheral blood due to spontaneous or therapeutic operations that fall off from solid tumor lesions (primary tumors, metastases) , a small number can escape and develop into metastases, increasing the risk of death in patients with malignant tumors. It is the main reason for the vast majority of patients with malignant tumors to experience distant metastasis and recurrence after surgery. Studies have shown that the number of CTCs in the blood is closely related...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09B82Y30/00D01D5/00D06M13/463D06M101/16
Inventor 裴仁军孙娜
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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