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Immune cell freezing medium and cryopreservation method

A technology of immune cells and cryopreservation, applied in the field of immune cell cryopreservation and cryopreservation, can solve the problems of low survival rate of immune cell recovery and poor cryopreservation efficiency, so as to improve the survival rate of recovery, prolong the survival period, reduce the lost effect

Inactive Publication Date: 2016-07-27
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method that can improve the cryopreservation efficiency of immune cells and improve the recovery and survival rate of immune cells in view of the defects of poor cryopreservation efficiency and low recovery survival rate of immune cells in the existing immune cell cryopreservation technology. Immune Cell Cryopreservation Solution

Method used

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  • Immune cell freezing medium and cryopreservation method

Examples

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Effect test

Embodiment 1

[0047] The cryopreservation method of microencapsulated immune cells provided by the present invention comprises the following steps:

[0048] S1a, obtaining immune cells—CIK cells;

[0049] S11a, isolating mononuclear cells from blood;

[0050] S12a, mix the mononuclear cell suspension with 1000mm 3 1 x 10 per uncoagulated fibrin gel 4 After mixing at a ratio of 1:1, place it in a 37°C incubator until the fibrin gel is completely solidified, and then place the solidified fibrin gel with mononuclear cells in 50ng / ml of CD3McAb and 10g / ml of RetroNectin. In the coated culture flask, and add the GTT551 medium containing the PPP of 1% volume fraction and the IFN-γ of 1000IU / ml concentration;

[0051] S13a, on the second day, add IL-1α and IL-2, and make their final concentrations in the GTT551 medium in step S12a respectively 100IU / ml and 300IU / ml;

[0052] S14a, on the third day, supplement the GTT551 medium in step S12a to a volume of 100ml, add IL-2 at the same time, and m...

Embodiment 2

[0069] The difference from Example 1 is that in this example, the cell cryopreservation medium used includes AIM-V medium, dimethyl sulfoxide with a volume fraction of 5%, and human serum with a volume fraction of 5%. .

Embodiment 3

[0071] The difference from Example 1 is that in this example, the cell cryopreservation medium used includes AIM-V medium, dimethyl sulfoxide with a volume fraction of 15% and human serum with a volume fraction of 15% .

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Abstract

The invention relates to an immune cell freezing medium.The immune cell freezing medium comprises dimethyl sulfoxide, human serum and an AIM-V culture medium.According to the immune cell freezing medium, the recovery survival rate of immune cells can be increased, the cell activity is kept, the survival time of the immune cells is prolonged, and losses of surface antigens of the immune cells are reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immune cell cryopreservation solution and a cryopreservation method. Background technique [0002] Cellular immunotherapy is an emerging and brand-new anti-tumor treatment method with significant curative effect. It makes up for the disadvantages of traditional surgery, radiotherapy and chemotherapy. It has been recognized as the most active and effective tumor comprehensive treatment model in the 21st century. It is a promising treatment method, and it is also the only treatment method in the world that has the hope of completely eradicating tumor cells. [0003] Immune cells refer to cells involved in or related to immune responses, including lymphocytes, dendritic cells, monocytes / macrophages, granulocytes, mast cells, etc. Immune cells can be divided into many types, and various immune cells play important roles in the human body. Mononuclear cells isolated from blood includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/0783C12N5/0781C12N5/0784C12N5/0786C12N5/078
CPCA01N1/0231A01N1/0284C12N5/0634C12N5/0635C12N5/0636C12N5/0637C12N5/0638C12N5/0639C12N5/064C12N5/0645C12N5/0646
Inventor 曾宪卓曾明哲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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