Application of 2-bromide-isovanillin for the manufacture of a medicament for anti-cancer or/and radiation/chemotherapy sensitization
a technology of isovanillin and bromide, which is applied in the field of compounds, can solve the problems of serious endangerment of human health and no effective treatment or prophylaxis yet available, and achieves the effect of improving the safety and efficacy of the produ
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example 1
Screening of Vanillin Derivatives Exhibiting a Remarkable Inhibitory Effect on the Proliferation of Cancer Cells
1. Effect of Vanillin on the Proliferation of Cancer Cells as Measured by MTT Assay
[0032]By way of example, human cervical cancer cell line HeLa was used to study the effect of vanillin on the proliferation of cancer cells by MTT assay. Specifically, human cervical cancer cells HeLa were seeded into a 96-well plate at a density of 5×103 cells / well, and were divided into four groups. To each of the groups vanillin was added at a concentration of 0, 100, 250 or 500 μmol / L, respectively, and was allowed to grow for 4 days. Using the MTT assay, the optical density (OD) values at 490 nm were measured and recorded every day, wherein the OD value is indicative of the number of cells, i.e., the proliferation of cells. The results, as shown in FIG. 1A, indicated that vanillin effectively inhibited the proliferation of cancer cells at a concentration above 250 μmol / L.
2. Effect of Va...
example 2
2-bromo-isovanillin Inhibited Proliferation and Induced Apoptosis in Cancer Cells
[0036]The effect of 2-bromo-isovanillin in inhibiting proliferation and inducing apoptosis in solid tumor cells of different tissue origins was investigated by clonogenic assay as follows:
1. Determination of the Killing of Cancer Cells by 2-Bromo-Isovanillin for 6 Days
[0037]By way of example, human cervical cancer cell line HeLa, lung cancer cell line A549, and ovarian cancer cell lines A2780 and HOC8 were used to determine the killing of cancer cells by 2-bromo-isovanillin for 6 days. Specifically, four groups were formed according to the type of the cancer cells. The cancer cells were diluted as appropriate, and were respectively plated into Petri dishes with a diameter of 60 cm, the amount of the cells adjusted depending on the concentration of 2-bromo-isovanillin to be added. Once adherence was achieved, each cell type received dosages of 0, 5, 10, 15, 20, 25, 30 and 35 μmol / L in triplicates. After ...
example 3
Determination of the Radiosensitizing Activity of 2-bromo-isovanillin
[0039]By way of example, human cervical cancer cell line HeLa was used to determine the radiosensitizing activity of 2-bromo-isovanillin.
[0040]Specifically, human cervical cancer cells HeLa were divided into six groups, diluted as appropriate, and plated into Petri dishes with a diameter of 60 cm. The amount of cells plated in each Petri dish was 300 / 500 for 0 Gy group (without Co-60 γ radiation), or 400 / 800 for 2 Gy group. Once adherence was achieved, three groups were treated with 20 μmol / L 2-bromo-isovanillin (BVAN3208) for 0, 4 and 18 hrs, respectively and then irradiated with Co-60 γ ray at 2 Gy, followed by two weeks under conventional culturing conditions. As to the other three groups, one group was treated with 20 μmol / L 2-bromo-isovanillin (BVAN3208) for 72 hrs and then cultured for two weeks under conventional conditions, one group received irradiation with Co-60 γ ray at 2 Gy and then was cultured for tw...
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