Dendritic cell (DC) tumor vaccine and preparation method thereof

A technology of dendritic cells and tumor vaccines, applied in the field of biological vaccines and their preparation, can solve the problems of increased drug resistance, high recurrence and metastasis rates of tumors

Inactive Publication Date: 2012-11-28
北京清美联创干细胞科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Dendritic cells targeting tumor cells thus obtained are used to treat cancer, but there are problems of increased drug resistance, high recurrence and metastasis rates of tumors after chemotherapy

Method used

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  • Dendritic cell (DC) tumor vaccine and preparation method thereof
  • Dendritic cell (DC) tumor vaccine and preparation method thereof

Examples

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Embodiment Construction

[0030] For further illustrating the present invention, specifically illustrate in conjunction with following examples:

[0031] In this example, the preferred breast tumor tissue is used as the source of stem cells, and the prepared DC tumor vaccine is used to prepare drugs for the treatment of breast tumor diseases, but the present invention is not limited to breast tumors, and can be prepared using any other tumor tissue as the source of stem cells. The process and therapeutic effect are similar to this embodiment or can be obtained by those skilled in the art through limited trials.

[0032] 1. Isolation and culture method of breast cancer stem cells

[0033] The tumor tissue was washed with normal saline containing antibiotics, cut into pieces with ophthalmic surgical scissors, digested with enzymes at 37°C for 2 hours, and filtered with a cell sieve, and the cell suspension was filtered with Stemedia TM WIT-I TM Culture Medium (stemgent) culture.

[0034] This medium...

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Abstract

The invention relates to dendritic cell (DC) tumor vaccine in which a multi-medicine resistance tumor stem cell antigen composition is loaded. The antigen composition loaded in the DC tumor vaccine derives from tumor tissues, and is mainly used for tumor stem cells; and specifically, the tumor tissues are subjected to tumor stem cell primary separation and culture so as to obtain the tumor stem cells, the tumor stem cells are screened by a flow cytometry so as to obtain tumor stem cells with multi-medicine resistance, and finally full cell antigens are prepared.

Description

technical field [0001] The invention relates to a biological vaccine and a preparation method thereof, in particular to a tumor vaccine and a preparation method thereof. Background technique [0002] Conventional tumor treatment methods include surgery, radiation therapy, and chemotherapy. However, the existing tumor treatment methods often cannot solve the problem of tumor recurrence. The main reason is that the cells that make up the tumor contain tumor stem cells, which have the biological characteristics of stem cells, that is, self-renewal, multipotential differentiation, and high tolerance, that is, high resistance to chemotherapy drugs. Therefore, tumor stem cells are the real culprits in the occurrence and development of tumors. [0003] Dendritic cells (DC, Dendritic Cell) are the most powerful professional antigen-presenting cells in the human body, and have the function of activating naive T cells. In layman's terms, DC is equivalent to a messenger, which trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00
Inventor 田杰
Owner 北京清美联创干细胞科技有限公司
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