34results about How to "Rapid value-added" patented technology

The invention relates to a biological and ecological multiplication reaction system. The biological and ecological multiplication reaction system is composed of a pre-treatment device, an ecological multiplication reactor and a vertical type bio-film reactor, which are connected in sequence, wherein the ecological multiplication reactor is composed of a tank body, a water flow driving device and an algae harvesting mechanism; a fluidized bed and alga, which are driven by the water flow driving device to flow along with sewage, are arranged in the tank body; the vertical type bio-film reactor comprises a shell, and an anaerobic region, an aerobic region, a reflowing region, a mud discharging region and a film water production region which are arranged in the shell; an integrated separation layer is arranged at the peripheries of the anaerobic region and the aerobic region; an annular region between the separation layer and a barrel body forms the reflowing region; a water outlet region is arranged at the top of the separation layer; a continuous or discontinuous dielectrophoresis electrode DEP (Diethyl Phthalate) film is arranged on an inner surface of the barrel body corresponding to the position of the water outlet region along the peripheral direction. According to the biological and ecological multiplication reaction system, the sewage is subjected to dual purification of the ecological multiplication reactor and the vertical type bio-film reactor; the biological and ecological multiplication reaction system has the advantages of enhancing removal effects of pollution substances including BOD (Biochemical Oxygen Demand), TN (Total Nitrogen), TP (Total Phosphorus) and the like, saving energy and reducing consumption and occupying a small area.

The invention discloses a production method of a microbial flora for processing ammonia containing wastewater. A microbial flora is placed in a biological reactor to carry out two grade amplification culture. For the first grade amplification culture, ammonium salts and a microbe growth promoter are added in batches. For the second grade amplification culture, at first, ammonium salts are added in batches, then water is changed, ammonium salt addition and water change are carried out alternatively; during ammonium salt addition process, when the concentration of nitration product reaches 3000-6000 mg / L, the water is changed in batches, at the same time, a microbe growth promoter is added; the promoter comprises 40 to 100 parts by weight of metal salts, 5 to 30 parts by weight of polyamine, and 0.5 to 15 parts by weight of inorganic acid hydroxylamine; the metal salts is composed of calcium salts, copper salts, magnesium salts, and / or ferrous salts; and after two grade amplification culture, the bacteria are collected. The production method has the advantages that the culture period is short, the culture efficiency is high, the activity of obtained microbial flora is high, the tolerance is strong, and the ammonia nitrogen containing wastewater can be effectively processed.

The invention provides a culture medium of Antrodia camphorate and a preparation method and application of the culture medium. The culture medium comprises the following components: in parts by weight, 180-250 parts of a carbon source, 10-20 parts of a nitrogen source, 0.5-2 parts of inorganic salt, 0.1-0.6 part of an induction factor, 0.05-0.15 part of vitamin B, 10-30 parts of agar and 650-850 parts of water. The invention provides the culture medium and the preparation method and application of the culture medium, and the culture medium has a reasonable formula; selection of the carbon source and nitrogen source are beneficial to rapid growth of Antrodia Camphorate during whole growth, and meanwhile through synergistic interaction of the added induction factor and the carbon source andnitrogen source, rapid artificial culture of Antrodia camphorate is achieved.

The invention discloses a mouse embryo stem cell culturing method and specific cultivating base, which cultures embryo stem cell on the trophoblast cell, wherein the trophoblast cell is epithelial cell or endothelium cell; the cultivating base consists of epithelial cell or endothelium cell and culturing liquid of embryo stem cell, which is high-sugar DMEM with 10-20% embryo cow serum or calf serum with 1. 5-3. 0*10-4mol / L Monothioglycerol, MTG.

The invention discloses a production method of a microbial flora for processing ammonia containing wastewater. A microbial flora is placed in a biological reactor to carry out two grade amplification culture. For the first grade amplification culture, ammonium salts and a microbe growth promoter are added in batches. For the second grade amplification culture, at first, ammonium salts are added in batches, then water is changed, ammonium salt addition and water change are carried out alternatively; during ammonium salt addition process, when the concentration of nitration product reaches 3000-6000 mg / L, the water is changed in batches, at the same time, a microbe growth promoter is added; the promoter comprises 40 to 100 parts by weight of metal salts, 5 to 30 parts by weight of polyamine, and 0.5 to 15 parts by weight of inorganic acid hydroxylamine; the metal salts is composed of calcium salts, copper salts, magnesium salts, and / or ferrous salts; and after two grade amplification culture, the bacteria are collected. The production method has the advantages that the culture period is short, the culture efficiency is high, the activity of obtained microbial flora is high, the tolerance is strong, and the ammonia nitrogen containing wastewater can be effectively processed.

The invention discloses a purple potato noodle with a high anthocyanin preservation rate and a production method thereof. The method comprises the following process steps: after the purple potato is fermented twice successively, it is mixed with gluten powder, sweet potato polyphenols, pueraria flavonoids, high gluten The flour is mixed, and the noodles are kneaded, waked up, pressed, cut, and dried to obtain purple potato noodles. The purple sweet potato noodles of the present invention fully retain the content and activity of anthocyanins in the purple sweet potato raw material under the premise of maintaining the cooking quality and mouthfeel quality of the noodles, thereby improving the nutritional quality of the purple sweet potato noodles. In addition, after the purple potato raw material is fermented, in addition to anthocyanin, it also fully frees functional components such as dehydroepiandrosterone and pectin, which endows purple potato noodles with various functions such as enhancing immunity, preventing cardiovascular system diseases, and preventing cancer. Nutritious and health-care effects provide high-quality purple potato noodle products for the market and consumers.

The invention discloses a method for separating and culturing rabbit fat stem cells. It includes the following steps: (1) aseptically obtain rabbit adipose tissue; (2) prepare a small piece of fat tissue; (3) digest the adipose tissue with trypsin, and separate the upper layer solution and the lower layer precipitate; (4) the upper layer solution Digest by time gradient method; (5) collect adipose stem cells, mix with adipose stem cell culture medium, inoculate and culture; (6) in vitro culture of adipose stem cells; (7) detect and freeze. In the cell separation method, the tissue digestion is not complete enough, and the cell separation efficiency is low. The separation method of adipose stem cells is optimized, which makes the adipose tissue digestion more thorough, improves the cell separation efficiency, and thus provides a better source of adipose stem cells.

The present invention relates to the field of vaccines, in particular, to a novel coronavirus, a cultivation method thereof, and an inactivated vaccine of the novel coronavirus. The novel coronavirus was deposited in the China Center for Type Culture Collection on July 28, 2020, with the preservation number: CCTCC NO:V202039, and the strain name is 2019‑nCoV / K‑T13. The present invention provides a strain of novel coronavirus, which has high culture titer, fast multiplication, and good passage stability. Even if the strain has been passed on for more than 18 generations, the sequence of the antigenic determinant (RBD) region of the strain is still not obvious. Therefore, it is suitable for large-scale cultivation of viruses and is suitable for use as vaccine strains. The present invention also determines the optimal inactivation condition of this strain, and obtains the novel coronavirus inactivated vaccine for the first time.

The invention provides a method for producing ethanol through co-fermenting C5 and C6 by using microbes. The method comprises the steps of enlarged culture of the microbes, and oxygen supply mode adjusting fermentation. The method for producing ethanol through co-fermenting C5 and C6 by using microbes allows different environment conditions to be controlled in different stages of the ethanol production process using a microbe fermentation medium and especially the difference of oxygen supply environments to be controlled in stages in order to realize high efficiency production of ethanol through co-fermenting C5 and C6 by using microbes, so the content of ethanol in a fermentation solution is high.

The invention relates to a treatment method for hardly-degradable organic nitrogen-containing wastewater. The treatment method employs an anoxic unit, an aerobic unit and a denitrification unit, wherein nitrosation dominant bacterial communities and a microbial growth promoter I are fed into the aerobic unit to control the nitrosation rate of effluent to be in a range of 65% to 80%; a part of theeffluent of the aerobic unit flows back to the anoxic unit, and a reflux ratio is controlled to be in a range of 15% to 25%; the other part of the effluent enters the denitrification unit, and denitrifying granular sludge and a microbial growth promoter II are fed into the denitrification unit; the microbial growth promoter I comprises a metal salt, a polyamine substance, organic acid hydroxylamine and Na2SO3; and the microbial growth promoter II comprises glycolipid, sugar alcohol and an organic acid salt. The treatment method achieves the purpose of advanced treatment of COD and total nitrogen by feeding the nitrosation dominant bacterial communities, the denitrifying granular sludge and the growth promoters and promoting the synergism of the bacterial communities in virtue of controlling the reflux ratio of the aerobic unit; and the method has the characteristics of high treatment efficiency, low cost, low reflux ratio, no secondary pollution and so on.

The invention discloses a biological treatment method for organic wastewater hard to degrade. The biological treatment method includes an anerobic unit, an aerobiotic unit and a denitrification unit. Nitrosation dominant bacteria and a nitrite bacterium growth accelerant are put in the aerobiotic unit, and the nitritation rate in discharged water is controlled to range from 65% to 80%, wherein one part of the discharged water of the aerobiotic unit flows back to the anerobic unit, the reflux ratio is controlled to range from 25% to 50%, and the other part of the discharged water of the aerobiotic unit enters the denitrification unit and is discharged after being treated through the denitrification unit. The growth accelerant comprises metal salt, polyamine type substances, organic acid hydroxylamine and Na2SO3, wherein the metal salt is composed of calcium salt, copper salt, magnesium salt and / or ferrous salt. The synergistic effect between florae is brought into play by adding the growth accelerant and regulating the reflux ratio of the discharged water of the aerobiotic unit, and therefore treatment for making COD and total nitrogen in the wastewater both reach the standard is achieved. The method has the beneficial effects of being short in flow, capable of reducing land occupation, low in reflux ratio, small in bacterium addition amount, stable in control, high in treatment efficiency, and the like.

The invention relates to a method for controlling a nitrification reaction progress in a sewage biochemical treatment process. Ammonia-containing sewage is subjected to nitrogen removal treatment by adopting a biochemical method; a nitrite bacteria growth promoter is added in the aerobic nitrification process after biochemical treatment; the growth promoter comprises metal salt, polyamine, organic acid hydroxylamine and Na2SO3, wherein the metal salt is 40-100 parts by weight; the polyamine is 5-30 parts by weight; the organic acid hydroxylamine is 0.05-1.5 part by weight; the Na2SO3 is 10-40 parts by weight; the metal salt comprises calcium salt, copper salt, magnesium salt and / or ferrous salt. The method provided by the invention has the benefit that as a specific composition and ratio of nitrite bacteria growth promoter is added in the wastewater aerobic nitrification process, so that the nitrification reaction progress can be stably controlled, and the nitrogen removal by shortcut nitrification and denitrification is really realized.

The invention discloses biological anaerobic reaction equipment for textile printing and dyeing wastewater treatment and a use method thereof, and particularly relates to the field of textile production. The equipment comprises an anaerobic reaction box, a sealing box cover is arranged on the top surface of the anaerobic reaction box, and a nitrogen charging protection mechanism is fixedly mounted on one side of the anaerobic reaction box; wherein a deoxidizing liquid distributor is fixedly mounted on the bottom surface of an inner cavity of the anaerobic reaction box, a preheating mechanism, a microbial reaction mechanism and a condensation plate are fixedly mounted in the anaerobic reaction box, and the preheating mechanism, the microbial reaction mechanism and the condensation plate are sequentially arranged in the anaerobic reaction box from bottom to top. According to the scheme, the anaerobic reaction equipment is provided with a novel reaction grid block structure, and a plurality of biological reaction grids arranged on the surfaces of the reaction grid blocks are used for attachment of microorganisms, so that good conditions are created for active biological proliferation, the activity of the microorganisms in the reactor is high, the oxygen content is reduced by filling nitrogen, and the biological proliferation is quick; and the reaction efficiency can be effectively improved.