34results about How to "Rapid value-added" patented technology

The invention relates to dendritic cell (DC) tumor vaccine in which a multi-medicine resistance tumor stem cell antigen composition is loaded. The antigen composition loaded in the DC tumor vaccine derives from tumor tissues, and is mainly used for tumor stem cells; and specifically, the tumor tissues are subjected to tumor stem cell primary separation and culture so as to obtain the tumor stem cells, the tumor stem cells are screened by a flow cytometry so as to obtain tumor stem cells with multi-medicine resistance, and finally full cell antigens are prepared.

The invention provides a method for producing ethanol through co-fermenting C5 and C6 by using microbes. The method comprises the steps of enlarged culture of the microbes, and oxygen supply mode adjusting fermentation. The method for producing ethanol through co-fermenting C5 and C6 by using microbes allows different environment conditions to be controlled in different stages of the ethanol production process using a microbe fermentation medium and especially the difference of oxygen supply environments to be controlled in stages in order to realize high efficiency production of ethanol through co-fermenting C5 and C6 by using microbes, so the content of ethanol in a fermentation solution is high.

The invention relates to a treatment method for hardly-degradable organic nitrogen-containing wastewater. The treatment method employs an anoxic unit, an aerobic unit and a denitrification unit, wherein nitrosation dominant bacterial communities and a microbial growth promoter I are fed into the aerobic unit to control the nitrosation rate of effluent to be in a range of 65% to 80%; a part of theeffluent of the aerobic unit flows back to the anoxic unit, and a reflux ratio is controlled to be in a range of 15% to 25%; the other part of the effluent enters the denitrification unit, and denitrifying granular sludge and a microbial growth promoter II are fed into the denitrification unit; the microbial growth promoter I comprises a metal salt, a polyamine substance, organic acid hydroxylamine and Na2SO3; and the microbial growth promoter II comprises glycolipid, sugar alcohol and an organic acid salt. The treatment method achieves the purpose of advanced treatment of COD and total nitrogen by feeding the nitrosation dominant bacterial communities, the denitrifying granular sludge and the growth promoters and promoting the synergism of the bacterial communities in virtue of controlling the reflux ratio of the aerobic unit; and the method has the characteristics of high treatment efficiency, low cost, low reflux ratio, no secondary pollution and so on.

The invention discloses a treatment method for difficultly-degraded organic wastewater. According to the treatment method, an anoxic unit, an aerobic unit and a denitrification unit are arranged, a nitrosation dominant flora and a microorganism growth promoter D are poured into the aerobic unit and the nitrosation rate in the effluent is controlled at 65-80%, one part of the effluent of the aerobic unit refluxes to the anoxic unit and the reflux ratio is controlled at 15-25%, the other part of the effluent of the aerobic unit enters the denitrification unit, denitrification particle sludge and a microorganism growth promoter E are poured into the denitrification unit, treatment is performed with the denitrification unit so as to discharge, the promoter D comprises a metal salt, a polyamine substance, organic acid hydroxylamine and Na2SO3, the promoter E comprises a metal salt, a polyamine substance, organic acid hydroxylamine and an organic acid salt, and the metal salt comprises a calcium salt, a copper salt, a magnesium salt and / or a ferrous salt. According to the present invention, the nitrosation dominant flora and the microorganism growth promoter D are poured into the aerobic unit to control the nitrosation rate, and the denitrification particle sludge and the microorganism growth promoter E are poured into the denitrification unit so as to achieve the deep treatment of the COD and the total nitrogen.

The invention relates to a biological and ecological multiplication reaction system. The biological and ecological multiplication reaction system is composed of a pre-treatment device, an ecological multiplication reactor and a vertical type bio-film reactor, which are connected in sequence, wherein the ecological multiplication reactor is composed of a tank body, a water flow driving device and an algae harvesting mechanism; a fluidized bed and alga, which are driven by the water flow driving device to flow along with sewage, are arranged in the tank body; the vertical type bio-film reactor comprises a shell, and an anaerobic region, an aerobic region, a reflowing region, a mud discharging region and a film water production region which are arranged in the shell; an integrated separation layer is arranged at the peripheries of the anaerobic region and the aerobic region; an annular region between the separation layer and a barrel body forms the reflowing region; a water outlet region is arranged at the top of the separation layer; a continuous or discontinuous dielectrophoresis electrode DEP (Diethyl Phthalate) film is arranged on an inner surface of the barrel body corresponding to the position of the water outlet region along the peripheral direction. According to the biological and ecological multiplication reaction system, the sewage is subjected to dual purification of the ecological multiplication reactor and the vertical type bio-film reactor; the biological and ecological multiplication reaction system has the advantages of enhancing removal effects of pollution substances including BOD (Biochemical Oxygen Demand), TN (Total Nitrogen), TP (Total Phosphorus) and the like, saving energy and reducing consumption and occupying a small area.

The invention discloses a production method of a microbial flora for processing ammonia containing wastewater. A microbial flora is placed in a biological reactor to carry out two grade amplification culture. For the first grade amplification culture, ammonium salts and a microbe growth promoter are added in batches. For the second grade amplification culture, at first, ammonium salts are added in batches, then water is changed, ammonium salt addition and water change are carried out alternatively; during ammonium salt addition process, when the concentration of nitration product reaches 3000-6000 mg / L, the water is changed in batches, at the same time, a microbe growth promoter is added; the promoter comprises 40 to 100 parts by weight of metal salts, 5 to 30 parts by weight of polyamine, and 0.5 to 15 parts by weight of inorganic acid hydroxylamine; the metal salts is composed of calcium salts, copper salts, magnesium salts, and / or ferrous salts; and after two grade amplification culture, the bacteria are collected. The production method has the advantages that the culture period is short, the culture efficiency is high, the activity of obtained microbial flora is high, the tolerance is strong, and the ammonia nitrogen containing wastewater can be effectively processed.

The invention provides a culture medium of Antrodia camphorate and a preparation method and application of the culture medium. The culture medium comprises the following components: in parts by weight, 180-250 parts of a carbon source, 10-20 parts of a nitrogen source, 0.5-2 parts of inorganic salt, 0.1-0.6 part of an induction factor, 0.05-0.15 part of vitamin B, 10-30 parts of agar and 650-850 parts of water. The invention provides the culture medium and the preparation method and application of the culture medium, and the culture medium has a reasonable formula; selection of the carbon source and nitrogen source are beneficial to rapid growth of Antrodia Camphorate during whole growth, and meanwhile through synergistic interaction of the added induction factor and the carbon source andnitrogen source, rapid artificial culture of Antrodia camphorate is achieved.

The invention relates to a reaction system for biologically and ecologically improving water quality. The reaction system is composed of a preprocessing device, an ecological multiplication reactor and a stereometric biomembrane reactor which are sequentially connected, wherein the ecological multiplication reactor is composed of a trough body, a waterflow driving device and an alga collection mechanism; a fluid bed and alga which are driven to flow along with sewage stream are placed in the trough body; the stereometric biomembrane reactor comprises a shell, as well as an anaerobic zone, an aerobic zone, a backflow zone, a spoil disposal zone and a membrane water producing zone which are arranged in the shell; the peripheries of the anaerobic zone and the aerobic zone are provided with an integrated separation layer; the backflow zone is formed in an annular region between the separation layer and a barrel body; the top of the separation layer is provided with a water discharge zone; the inner surface, which is corresponding to the water discharge zone, of the barrel body is peripherally provided with a continuous or discontinuous dielectrophoresis (DEP) electrode membrane. By virtue of the reaction system for biologically and ecologically improving the water quality, the sludge is dually purified by virtue of the ecological multiplication reactor and the stereometric biomembrane reactor; the reaction system has the advantages of enhanced removal effect on pollutants such as biological oxygen demand (BOD), total nitrogen (TN) and total phosphorus (TP), energy conservation and consumption reduction, and small occupied area.

The invention discloses a biological treatment method for organic wastewater hard to degrade. The biological treatment method includes an anerobic unit, an aerobiotic unit and a denitrification unit. Nitrosation dominant bacteria and a nitrite bacterium growth accelerant are put in the aerobiotic unit, and the nitritation rate in discharged water is controlled to range from 65% to 80%, wherein one part of the discharged water of the aerobiotic unit flows back to the anerobic unit, the reflux ratio is controlled to range from 25% to 50%, and the other part of the discharged water of the aerobiotic unit enters the denitrification unit and is discharged after being treated through the denitrification unit. The growth accelerant comprises metal salt, polyamine type substances, organic acid hydroxylamine and Na2SO3, wherein the metal salt is composed of calcium salt, copper salt, magnesium salt and / or ferrous salt. The synergistic effect between florae is brought into play by adding the growth accelerant and regulating the reflux ratio of the discharged water of the aerobiotic unit, and therefore treatment for making COD and total nitrogen in the wastewater both reach the standard is achieved. The method has the beneficial effects of being short in flow, capable of reducing land occupation, low in reflux ratio, small in bacterium addition amount, stable in control, high in treatment efficiency, and the like.

The invention relates to the field of vaccines, in particular to a novel coronavirus and a culture method thereof as well as a novel coronavirus inactivated vaccine. The novel coronavirus was preserved in China Center for Type Culture Collection on July 28th, 2020, with the preservation number of CCTCC NO: V202039 and the strain name of 2019-nCoV / K-T13. The invention provides a strain of the novelcoronavirus, the strain has high culture titer, fast proliferation and good passage stability, and the antigenic determinant (RBD) regional sequence of the strain is still not changed obviously evenif the strain is passed for more than 18 generations, so that the strain is suitable for large-scale culture of viruses and is suitable for being used as a vaccine strain. The optimal inactivation condition of the strain is further determined, and the novel coronavirus inactivated vaccine is obtained for the first time.

The invention relates to the field of medical treatment, in particular to a method of improving the killing effect of CIK cells on cancer cells. The method of improving the killing effect of the CIK cells on the cancer cells specifically comprises the following steps: in the process of inducing the CIK cells, a CD3 monoclonal antibody, interleukin-1, interleukin-2, interferon IFN-gamma and interleukin-24 are added. By means of the method of improving the killing effect of the CIK cells on the cancer cells, the effect-target ratio is increased, and the killing activity to tumor cells is promoted.

The invention discloses a serum-free cell culture medium additive, a culture medium and application. The serum-free cell culture medium additive is rich in cell factors and growth factors, replaces growth factors, adherent factors and the like contained in serum, and guarantees normal growth of mesenchymal stem cells withtout serum. Experiments prove that the culture medium can replace various commercially available mesenchymal stem cell culture media, and has an excellent expression effect on the culture of mesenchymal stem cells. The culture medium added with the additive can enable mesenchymal stem cells to be subjected to multi-generation amplification in an ideal nutrition balance state without differentiation (within at least 10 generations).

The invention relates to a method for controlling a nitrification reaction progress in a sewage biochemical treatment process. Ammonia-containing sewage is subjected to nitrogen removal treatment by adopting a biochemical method; a nitrite bacteria growth promoter is added in the aerobic nitrification process after biochemical treatment; the growth promoter comprises metal salt, polyamine, organic acid hydroxylamine and Na2SO3, wherein the metal salt is 40-100 parts by weight; the polyamine is 5-30 parts by weight; the organic acid hydroxylamine is 0.05-1.5 part by weight; the Na2SO3 is 10-40 parts by weight; the metal salt comprises calcium salt, copper salt, magnesium salt and / or ferrous salt. The method provided by the invention has the benefit that as a specific composition and ratio of nitrite bacteria growth promoter is added in the wastewater aerobic nitrification process, so that the nitrification reaction progress can be stably controlled, and the nitrogen removal by shortcut nitrification and denitrification is really realized.

The invention discloses a preparation method of a red date enzyme, adopting enzymolysis-coupling fermentation production technology. The preparation method comprises the following steps: (1) pulp preparation: cleaning selected red dates and carrying out pulp preparation to obtain red date pulp; (2) enzymolysis: adjusting the red date pulp to a pH value of 5.0-6.5 and a temperature of 25-50 DEG C,adding a complex enzyme at least consisting of rhamnosan degrading enzyme, pectinase, dextranase and galactosidase, and carrying out enzymolysis for 1-3 hours; (3) coupling fermentation: preparing lactobacillus rhamnosus into bacterial suspension of 10<4>-10<8> CFU / mL by using sterile normal saline; when the enzymolysis lasts for 1-3 hours, inoculating the lactobacillus rhamnosus aseptically, stirring the mixture, carrying out static culture for 12-48 hours, and stopping fermentation when the pH value is 3.5-4.5; and (4) post-treatment: carrying out solid-liquid separation on the enzyme fermented in the step (3) to obtain liquid red date enzyme. The method disclosed by the invention adopts the enzymolysis-coupling fermentation production technology, thus improving the utilization rates ofred date components and the yields of the enzyme and prebiotics.

The invention relates to bacillus subtilis culture media, a preparation method thereof and a bacillus subtilis culture method. The bacillus subtilis culture media include a seed culture medium and a fermentation culture medium. Effective components of the fermentation culture medium include 10-30g / L of sugar, 5-25g / L of broken-wall beer yeast, 1-15g / L of hydrolyzed feather meal, 1-5g / L of peptone, 1-5g / L of protein hydrolysate and 0.1-0.6g / L of first additive. The preparation method of the bacillus subtilis culture media includes weighing the components according to the proportion, adding the components into carrier liquid such as water, and stirring the carrier liquid. The method of culturing bacillus subtilis by the bacillus subtilis culture media includes seed-phase culture, fermentation-phase culture, post-treatment and detection. The bacillus subtilis culture media have rich nutritional ingredients, and high-density thalli and high-yield iturin can be obtained without later material supplement. The fermentation process is simple, convenience in operation is achieved, and mass fermentation and production of the iturin are benefited.

The invention discloses a fermentation method for promoting high yield of oil of rhizopus arrhizus. The method is characterized by firstly regulating and controlling the dissolved oxygen amount in a process of fermentation culture in a segmented way, wherein the higher dissolved oxygen amount at the early stage of fermentation is beneficial to the biomass accumulation of the rhizopus arrhizus, andthe lowering of the dissolved oxygen amount at the later stage of fermentation is beneficial to oil accumulation; secondly, in the stage of oil accumulation at the later stage of fermentation, addingsodium tungstate to promote oil synthesis in thallus of the rhizopus arrhizus to improve oil production. A formula of a fermentation culture medium involved in the invention comprises corn starch, glucose, ammonium nitrate, peanut meal zymolyte, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate, various trace elements, absolute methanol, melatonin, fulvic acid, 2-mercaptoethanol,4-chlorophenoxyacetic acid, N-methylmorpholine and sophorolipid, and the use of the culture medium has an obvious promoting effect on the improvement of the biomass and oil production of the rhizopusarrhizus. The method disclosed by the invention has the advantages that the operation is relatively simple, the production cost can be reduced, and the method is easy to apply to industrial production.

The invention discloses a method for in vitro expanding tumor stem cells. The invention aims to solve the problem that the stem cell quality is poor according to a traditional method for in vitro expanding tumor stem cells. According to the technical scheme, the method for in vitro expanding tumor stem cells comprises the following steps: 1) taking a serum-free medium and adding 20-35mg / L B27, 10-25mg / L IFO and 1-3mg / L MPF to the serum-free medium; 2) increasing the temperature of the medium to 37 DEG C and sterilizing with ultraviolet rays; 3) transferring the tumor tissues into the medium under an aseptic condition and stirring along a same direction; 4) adding 0.5mg / L Accutase into the medium acquired in the step 3) every 4 hours, wherein 0.1mg / L Accutase is additionally added at each time and the stirring direction is reversely adjusted when the Accutase is added each time; 5) culturing for 20-35h and then centrifugally separating, thus obtaining the tumor stem cells. The quality of stem cells obtained according to the method for in vitro expanding tumor stem cells is higher.

The present invention relates to the technical field of lactic acid bacteria, and proposes a method for detecting cheese strains mixed with Lactobacillus plantarum, comprising the following steps: B1, shaking the cultivated bacterial powder culture to be tested so that the clot is fully broken to obtain B1 passage; B2, Dilute passage B1 with saline; B3, select 10 3 、10 4 、10 5 For three consecutive dilutions, each dilution draws a sample homogeneous solution and places it in a solid chromogenic medium for surface coating; B4, observe the colony morphology after anaerobic culture at 36°C±1°C for 48h±2h. Through the above technical solution, the problem in the prior art that it is impossible to detect cheese strains mixed with Lactobacillus plantarum is solved.

The invention discloses a traditional Chinese medicine foodstuff formula for feeding geese. The traditional Chinese medicine foodstuff formula comprises the following raw material components in percentage: 1.5% of elephantopus scaber, 3.5% of sticktight, 2% of vitex negundo, 4% of swamp mahogany, 6% of houttuynia cordata, 8% of honeysuckle, 18% of crushed corn particles, 12% of crushed wheat particles, 10% of degreased soybean dregs, 4% of fish meal, 6% of soybean meal powder, 0.25% of table salt and 24.75% of pasture. By matching carbohydrate-rich crushed corn particles, crushed wheat particles, defatted soybean residues, soybean meal and other raw materials in the ingredients, the digestion and absorption speed of geese is high, and herbal traditional Chinese medicines such as elephantopus scaber, sticktight, vitex negundo, swamp mahogany, houttuynia cordata and honeysuckle are added in proportion, so that beneficial bacteria in large intestines and cecum of the geese are rapidly increased. Meanwhile, fermented crude fibers are torn down, the amount of the B-group vitamins is increased, the fat and the meat are rapidly increased, the outbreak of goose plague and viral hepatitis is greatly avoided, the feeding food has the medicinal value, the quality of the fed goose meat is improved, the disease is prevented, and the safety is improved.

The invention discloses biological anaerobic reaction equipment for textile printing and dyeing wastewater treatment and a use method thereof, and particularly relates to the field of textile production. The equipment comprises an anaerobic reaction box, a sealing box cover is arranged on the top surface of the anaerobic reaction box, and a nitrogen charging protection mechanism is fixedly mounted on one side of the anaerobic reaction box; wherein a deoxidizing liquid distributor is fixedly mounted on the bottom surface of an inner cavity of the anaerobic reaction box, a preheating mechanism, a microbial reaction mechanism and a condensation plate are fixedly mounted in the anaerobic reaction box, and the preheating mechanism, the microbial reaction mechanism and the condensation plate are sequentially arranged in the anaerobic reaction box from bottom to top. According to the scheme, the anaerobic reaction equipment is provided with a novel reaction grid block structure, and a plurality of biological reaction grids arranged on the surfaces of the reaction grid blocks are used for attachment of microorganisms, so that good conditions are created for active biological proliferation, the activity of the microorganisms in the reactor is high, the oxygen content is reduced by filling nitrogen, and the biological proliferation is quick; and the reaction efficiency can be effectively improved.

The invention discloses purple sweet potato noodles with high anthocyanin preservation rate and a making method thereof, and the method comprises the following process steps: fermenting purple sweet potatoes twice, mixing the fermented purple sweet potatoes with vital gluten, sweet potato polyphenols, pueraria flavones and high gluten flour, and performing dough kneading, dough standing, dough pressing, dough cutting and drying to obtain the purple sweet potato noodles. According to the purple sweet potato noodles disclosed by the invention, the content and activity of anthocyanin in the purple sweet potato raw material are fully reserved on the premise of keeping the cooking quality, the taste quality and the like of the noodles, and the nutritional quality of the purple sweet potato noodles is improved. Besides, after the purple sweet potato raw material is fermented, besides anthocyanin, functional components such as dehydroepiandrosterone, pectin and the like are fully dissociated, so that the purple sweet potato noodles are endowed with various nutritional and health-care effects of enhancing immunity, preventing cardiovascular system diseases, preventing cancers and the like, and a high-quality purple sweet potato noodle product is provided for the market and consumers.