Bacillus subtilis culture media, preparation method thereof and bacillus subtilis culture method

A technology of Bacillus subtilis and cultivation methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems that cost control cannot be widely promoted, and achieve a wide range of market application value and prospects, rich in nutrients, The effect of simple fermentation process

Active Publication Date: 2017-03-15
广州市中添生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two methods cannot be widely promoted due to cost control and pollution control issues.

Method used

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  • Bacillus subtilis culture media, preparation method thereof and bacillus subtilis culture method

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Experimental program
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Effect test

Embodiment 1

[0037] A culture medium for cultivating high-yiurin-producing Bacillus subtilis, comprising a seed medium and a fermentation medium, the components of the fermentation medium and the contents of various components in water are:

[0038] Sucrose 10g / L, broken brewer's yeast 25g / L, hydrolyzed feather powder 1g / L, soybean peptone 5g / L, wheat hydrolyzed protein 1g / L, magnesium sulfate heptahydrate 0.5g / L and ferrous sulfate 0.05g / L.

Embodiment 2

[0040] A culture medium for cultivating high-yiurin-producing Bacillus subtilis, comprising a seed medium and a fermentation medium, the components of the fermentation medium and the contents of various components in water are:

[0041] Sucrose 30g / L, broken brewer's yeast 5g / L, hydrolyzed feather powder 15g / L, soybean peptone 1g / L, wheat hydrolyzed protein 5g / L, magnesium sulfate heptahydrate 0.1g / L and ferrous sulfate 0.1g / L.

Embodiment 3

[0043]A culture medium for cultivating high-yiurin-producing Bacillus subtilis, comprising a seed medium and a fermentation medium, the components of the fermentation medium and the contents of various components in water are:

[0044] Sucrose 15g / L, broken brewer's yeast 18g / L, hydrolyzed feather meal 6g / L, soybean peptone 4g / L, wheat hydrolyzed protein 3g / L, magnesium sulfate heptahydrate 0.5g / L and ferrous sulfate 0.05g / L.

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Abstract

The invention relates to bacillus subtilis culture media, a preparation method thereof and a bacillus subtilis culture method. The bacillus subtilis culture media include a seed culture medium and a fermentation culture medium. Effective components of the fermentation culture medium include 10-30g/L of sugar, 5-25g/L of broken-wall beer yeast, 1-15g/L of hydrolyzed feather meal, 1-5g/L of peptone, 1-5g/L of protein hydrolysate and 0.1-0.6g/L of first additive. The preparation method of the bacillus subtilis culture media includes weighing the components according to the proportion, adding the components into carrier liquid such as water, and stirring the carrier liquid. The method of culturing bacillus subtilis by the bacillus subtilis culture media includes seed-phase culture, fermentation-phase culture, post-treatment and detection. The bacillus subtilis culture media have rich nutritional ingredients, and high-density thalli and high-yield iturin can be obtained without later material supplement. The fermentation process is simple, convenience in operation is achieved, and mass fermentation and production of the iturin are benefited.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a culture medium of Bacillus subtilis with high yield of iturin, a preparation method thereof and a cultivation method of Bacillus subtilis. Background technique [0002] In my country, due to many reasons such as high animal breeding density, complex and diverse livestock and poultry diseases, and inadequate supervision, there are widespread problems of antibiotic abuse, such as excessive use of antibiotics in the prevention and treatment of livestock and poultry diseases, random addition of antibiotics to livestock and poultry feed, random The use of antibiotic drug filter residues and the use of prohibited antibiotics have made animal breeding and food safety problems increasingly serious, and the increase in bacterial resistance has also laid hidden dangers for the sustainable development of the breeding industry. At present, relevant departments and state agencies have i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/04C12R1/865
CPCC07K14/32C12N1/20C12P21/02
Inventor 张钧利周尧
Owner 广州市中添生物科技有限公司
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