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Method for producing ethanol through co-fermenting C5 and C6 by using microbes

A microbial and co-fermentation technology, applied in the field of microbial fermentation, can solve problems such as low conversion rate

Active Publication Date: 2015-07-29
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Therefore, the object of the present invention is to overcome the disadvantages of the differential consumption of fermentation supply in the process of microbial co-fermentation of C5 sugar and C6 sugar to produce ethanol in the prior art, and lead to too low conversion rate in actual production, thereby providing a Efficient use of microbial co-fermentation of C5 sugar and C6 sugar to produce ethanol fermentation process

Method used

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  • Method for producing ethanol through co-fermenting C5 and C6 by using microbes

Examples

Experimental program
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Embodiment 1

[0080] This embodiment provides an expanded culture method for co-fermenting C5 and C6 microorganisms to produce ethanol. Taking recombinant S. ) (such as Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae424A(LNH-ST), M.W.Lau, B.E.Dale, Proc Natl Acad Sci USA, 106(2009), pp.1368-1373; Two-step SSCF to convert AFEX-treated switchgrass to ethanol using commercial enzymes and Saccharomyces cerevisiae424A (LNH-ST), M.Jin, M.W.Lau, V.Balan, B.E.Dale, Bioresour Technol, 101(2010), pp.8171–8178, etc. report), for It is commercially available, and the expanded cultivation method adopts a first-stage expanded cultivation step.

[0081] Specifically include:

[0082] A. Seed liquid culture steps

[0083] Prepare YEPD medium, inoculate the preserved above bacteria into YEPD medium, control the temperature at 25°C, pH 6.0, rotation speed 200rpm, ventilation rate 0.2L / L / min, and cultivate for 12h until the concentration of the above bacteria is (0...

Embodiment 2

[0089] This example provides an expanded culture method for microorganisms that co-ferment C5 and C6 to produce ethanol. Taking Candida tropicalis as an example to carry out the expanded culture experiment, the Candida tropicalis is commercially available, and the expanded culture Methods A two-stage expanded culture step was adopted.

[0090] Specifically include:

[0091] A. Seed liquid culture steps

[0092] Prepare YEPD medium, inoculate the above-mentioned preserved bacteria into YEPD medium, control the temperature at 25°C, pH6.0, rotation speed 200rpm, ventilation rate 0.2L / L / min, and cultivate until the concentration of the above-mentioned bacteria is (0.1-0.5 ) x 10 9 a / mL;

[0093] The YEPD medium specifically contains 10 g / L of yeast powder, 20 g / L of peptone, and 20 g / L of glucose, and the pH is adjusted to 6.0.

[0094] B. Expand the cultivation steps

[0095] 1) Put the cultured seed solution into a 50L primary expansion tank containing a primary expansion m...

Embodiment 3

[0099]This embodiment provides an expanded culture method for co-fermenting C5 and C6 microorganisms to produce ethanol. Taking recombinant S. Tertiary scale-up cultivation steps.

[0100] Specifically include:

[0101] A. Seed liquid culture steps

[0102] Prepare YEPD medium, inoculate the preserved above-mentioned bacteria into YEPD medium, control the temperature at 30°C, pH6.0, ventilation rate 0.2L / L / min, rotation speed 200rpm, and cultivate for 14h until the concentration of the above-mentioned bacteria is (0.2 -0.3)×10 9 a / mL;

[0103] The YEPD medium specifically contains 10 g / L of yeast powder, 20 g / L of peptone, and 20 g / L of glucose, and the pH is adjusted to 6.5.

[0104] B. Expand the cultivation steps

[0105] 1) Put the cultured seed liquid into a 50L primary expansion tank containing a primary expansion medium at an inoculation amount of 10% (v / v), control the temperature at 30°C, pH 5.0, and rotate at 200rpm Ventilate the air, the ventilation rate is 0....

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Abstract

The invention provides a method for producing ethanol through co-fermenting C5 and C6 by using microbes. The method comprises the steps of enlarged culture of the microbes, and oxygen supply mode adjusting fermentation. The method for producing ethanol through co-fermenting C5 and C6 by using microbes allows different environment conditions to be controlled in different stages of the ethanol production process using a microbe fermentation medium and especially the difference of oxygen supply environments to be controlled in stages in order to realize high efficiency production of ethanol through co-fermenting C5 and C6 by using microbes, so the content of ethanol in a fermentation solution is high.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for producing ethanol by using microorganisms to co-ferment C5 and C6. Background technique [0002] The depletion of many non-renewable fossil energy sources such as oil has drawn more and more attention to renewable energy, especially biofuels, and has brought huge business opportunities and social significance. [0003] According to the relevant definitions of the International Energy Agency (IEA), conventional biofuels include sugar-based and starch-based ethanol, oil crop-based biodiesel, directly usable vegetable oils, and biogas produced through anaerobic digestion; Transformation technologies in R&D, pilot or demonstration stages are usually called second-generation technologies or third-generation technologies. This category includes hydrogenated vegetable oils (HVO) produced from animal fats and vegetable oils, as well as biofuels produced fro...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12R1/865C12R1/19C12R1/01C12R1/645C12R1/84C12R1/72C12R1/74C12R1/18C12R1/22C12R1/145C12R1/77
CPCC12P7/06Y02E50/10
Inventor 沈乃东李凡林鑫苏会波彭超熊强薛小舟林海龙李春玲袁敬伟刘文信
Owner COFCO NUTRITION & HEALTH RES INST
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