Serum-free cell culture medium additive, culture medium and application

A technology of serum-free medium and additives, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problems that are not specially suitable for mesenchymal stem cells, etc., to ensure normal growth, fast growth speed, avoid immune repelling effect

Inactive Publication Date: 2020-04-14
江苏艾洛特生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention patent with the patent application number 20181015233 discloses an additive for CHO cell serum-free medium, the additive is vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin H, pyridoxine hydrochloride, pyridoxal hydrochloride , inositol, folic acid, or a mixture of several of them; this invention studies the important vitamin supplements in serum-free medium, regulates cell growth metabolism and product expression, and adjusts for different cell lines and different products The nutrient components and the content of each nutrient component in the medium can increase the survival rate of cells and physiological activities such as proliferation, regulate the growth state of cells, thereby increase product yield, save costs, and obtain greater economic benefits, but this patent is not specifically applicable to Culture of Mesenchymal Stem Cells

Method used

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  • Serum-free cell culture medium additive, culture medium and application
  • Serum-free cell culture medium additive, culture medium and application
  • Serum-free cell culture medium additive, culture medium and application

Examples

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Embodiment 1

[0040] A serum-free medium supplement for cells, the supplement contains albumin, growth factors, glucose, sodium ions, triglycerides, calcium ions, magnesium ions, chloride ions, and phosphate ions.

[0041] Albumin concentration 10㎎ / ml, growth factor concentration 191.78ng / ml, glucose 3㎎ / ml, sodium ion 0.15mol / L, triglyceride 1.0mg / ml, calcium ion 0.7mg / ml, magnesium ion 22ug / ml, chloride ion 4.9mmol / L, phosphate ion 9mmol / L.

[0042] Growth factors include platelet-derived growth factor, vascular endothelial growth factor, epidermal growth factor, fibroblast growth factor, transforming growth factor. The concentration of platelet-derived growth factor is 64ng / ml, the concentration of vascular endothelial growth factor is 10ng / ml, the concentration of epidermal growth factor is 4.75ng / ml, the concentration of fibroblast growth factor is 8.88ng / ml, and the concentration of transforming growth factor is 104.15ng / ml ml. Its preparation method is common knowledge of those ski...

Embodiment 2

[0060] A serum-free medium supplement for cells, similar to the examples, except that the albumin concentration is 6㎎ / ml, the growth factor concentration is 191.78ng / ml, the glucose is 2㎎ / ml, the sodium ion is 0.1mol / L, Triglyceride 0.5mg / ml, calcium ion 0.4mg / ml, magnesium ion 15ug / ml, chloride ion 3mmol / L, phosphate ion 4mmol / L.

Embodiment 3

[0062] A serum-free medium supplement for cells, similar to the examples, except that the albumin concentration is 8㎎ / ml, the growth factor concentration is 191.78ng / ml, the glucose is 2.5㎎ / ml, and the sodium ion is 0.12mol / ml L, triglyceride 0.8mg / ml, calcium ion 0.6mg / ml, magnesium ion 20ug / ml, chloride ion 4.5mmol / L, phosphate ion 8mmol / L.

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Abstract

The invention discloses a serum-free cell culture medium additive, a culture medium and application. The serum-free cell culture medium additive is rich in cell factors and growth factors, replaces growth factors, adherent factors and the like contained in serum, and guarantees normal growth of mesenchymal stem cells withtout serum. Experiments prove that the culture medium can replace various commercially available mesenchymal stem cell culture media, and has an excellent expression effect on the culture of mesenchymal stem cells. The culture medium added with the additive can enable mesenchymal stem cells to be subjected to multi-generation amplification in an ideal nutrition balance state without differentiation (within at least 10 generations).

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a cell serum-free medium supplement, medium and application. Background technique [0002] At present, the potential market for stem cells has huge business opportunities, and stem cell research will have immeasurable medical value. The discovery of stem cells and the development of stem cell technology not only provide a unique perspective, method and means for the treatment of human diseases, but also provide new hope and dawn for the treatment of human diseases. Mesenchymal stem cells are a kind of adult stem cells, which have the commonality of stem cells and strong self-proliferation ability. Mesenchymal stem cells have good differentiation potential and can be used to repair the damage of specific tissues and organs; they can participate in the formation of a microenvironment that supports hematopoiesis, promote hematopoietic recovery, and co-transplantati...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N5/0663C12N5/0667C12N5/0668C12N2500/05C12N2500/12C12N2500/14C12N2500/16C12N2500/34C12N2500/36C12N2500/90C12N2501/11C12N2501/115C12N2501/135C12N2501/15C12N2501/165C12N2501/998
Inventor 刘会杰杨美家尹鸿萍张军华刘永军王岩
Owner 江苏艾洛特生物科技有限公司
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