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Culture medium of Antrodia camphorate and preparation method and application of culture medium

A culture medium, the technology of Antrodia cintrodia, applied in the biological field, can solve the problems of unstable quality of Antrodia cinnamomea, unfavorable nutrient intake, long cultivation period, etc., and achieve the effects of rapid artificial breeding, low investment in production equipment, and accelerated growth

Pending Publication Date: 2018-11-30
GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The carbon source and nitrogen source of the medium are not conducive to the nutrient uptake of the later culture, the culture period is long, the yield is low, and the quality of the obtained Antrodia camphorata is unstable

Method used

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  • Culture medium of Antrodia camphorate and preparation method and application of culture medium
  • Culture medium of Antrodia camphorate and preparation method and application of culture medium
  • Culture medium of Antrodia camphorate and preparation method and application of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A kind of culture medium of Antrodia camphorata comprises the following components in parts by weight:

[0057]

[0058] Training method:

[0059] (1) Mix the components of the medium evenly, adjust the pH to 7.2, autoclave at 121°C for 20 minutes, and put it at room temperature for later use;

[0060] (2) Using the solid dish culture method, inoculate the colony of Antrodia cinnamomea on the culture medium, and activate Antrodia cinnamomea in a dark incubator at 28°C for 5 days until white mycelia;

[0061] (3) Take 1cm of activated Antrodia camphorata 2 , inoculated in the middle of the culture medium, set up three repeated groups, placed in a constant temperature mold incubator at 28°C, and cultured in the dark for 7 days.

Embodiment 2

[0063] A kind of culture medium of Antrodia camphorata comprises the following components in parts by weight:

[0064]

[0065]

[0066] Training method:

[0067] (1) Mix the components of the medium evenly, adjust the pH to 7.3, autoclave at 121°C for 20 minutes, and put it at room temperature for later use;

[0068] (2) Using the solid dish culture method, inoculate the colony of Antrodia cinnamomea on the culture medium, and activate Antrodia cinnamomea in a dark incubator at 28°C for 5 days until white mycelia;

[0069] (3) Take 1cm of activated Antrodia camphorata 2 , inoculated in the middle of the culture medium, set up three repeated groups, placed in a constant temperature mold incubator at 26°C, and cultured in the dark for 10 days.

Embodiment 3

[0071] A kind of culture medium of Antrodia camphorata comprises the following components in parts by weight:

[0072]

[0073]

[0074] Training method:

[0075] (1) Mix the components of the medium evenly, adjust the pH to 7.4, autoclave at 121°C for 20 minutes, and put it at room temperature for later use;

[0076] (2) Using the solid dish culture method, inoculate the colony of Antrodia cinnamomea on the culture medium, and activate Antrodia cinnamomea in a dark incubator at 28°C for 5 days until white mycelia;

[0077] (3) Take 1cm of activated Antrodia camphorata 2 , inoculated in the middle of the culture medium, set up three repeated groups, placed in a constant temperature mold incubator at 30°C, and cultured in the dark for 15 days.

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Abstract

The invention provides a culture medium of Antrodia camphorate and a preparation method and application of the culture medium. The culture medium comprises the following components: in parts by weight, 180-250 parts of a carbon source, 10-20 parts of a nitrogen source, 0.5-2 parts of inorganic salt, 0.1-0.6 part of an induction factor, 0.05-0.15 part of vitamin B, 10-30 parts of agar and 650-850 parts of water. The invention provides the culture medium and the preparation method and application of the culture medium, and the culture medium has a reasonable formula; selection of the carbon source and nitrogen source are beneficial to rapid growth of Antrodia Camphorate during whole growth, and meanwhile through synergistic interaction of the added induction factor and the carbon source andnitrogen source, rapid artificial culture of Antrodia camphorate is achieved.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a culture medium of Antrodia camphorata and its preparation method and application. Background technique [0002] Antrodia Camphorate (Antrodia Camphorate) is a perennial fungus belonging to the family Polyporaceae of the order Polyporaceae, also known as Antrodia camphorata, Antrodia red, and Ganoderma lucidum. Antrodia Cinnamomea has a wide range of biological activities, such as detoxification and liver protection, anti-tumor, anti-oxidation, immune regulation, detoxification, etc. It is a medicinal fungus with great development potential and application prospects, which has attracted the attention of researchers at home and abroad in recent years. However, due to factors such as its slow growth and strong host specificity, the natural yield is seriously insufficient to meet the market demand. Therefore, obtaining Antrodia antrodia through artificial rapid propagation techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20A01G18/00
CPCA01G18/00A01G18/20
Inventor 沈小钟郑彦云崔穗旭汪小根张雷红刘卫海沈小莉邱蔚芬
Owner GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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