Production method of microbial flora for processing ammonia containing wastewater

Abstract

The invention discloses a production method of a microbial flora for processing ammonia containing wastewater. A microbial flora is placed in a biological reactor to carry out two grade amplification culture. For the first grade amplification culture, ammonium salts and a microbe growth promoter are added in batches. For the second grade amplification culture, at first, ammonium salts are added in batches, then water is changed, ammonium salt addition and water change are carried out alternatively; during ammonium salt addition process, when the concentration of nitration product reaches 3000-6000 mg / L, the water is changed in batches, at the same time, a microbe growth promoter is added; the promoter comprises 40 to 100 parts by weight of metal salts, 5 to 30 parts by weight of polyamine, and 0.5 to 15 parts by weight of inorganic acid hydroxylamine; the metal salts is composed of calcium salts, copper salts, magnesium salts, and / or ferrous salts; and after two grade amplification culture, the bacteria are collected. The production method has the advantages that the culture period is short, the culture efficiency is high, the activity of obtained microbial flora is high, the tolerance is strong, and the ammonia nitrogen containing wastewater can be effectively processed.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a production method of microbial flora for treating ammonia-containing wastewater, which can realize the industrialization and commercialization of ammonia nitrogen-removing microbial flora. Background technique [0002] Biological denitrification of wastewater includes two processes of nitrification and denitrification. The microorganisms involved in nitrification are mainly nitrifying bacteria, which are typical autotrophic bacteria that obtain energy from the process of biological oxidation of ammonia nitrogen to nitrate nitrogen. Generally, inorganic carbon is used as the Carbon source, so the metabolism time is long and the growth is relatively slow. It belongs to Gram-negative bacteria. The content of peptidoglycan in the cell wall is low, the content of protein and fat is high, and it is sensitive to environmental changes. Therefore, the adaptability ...

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Problems solved by technology

[0006] Although there are some technologies on the cultivation of nitrifying bacteria at present, the large-scale production efficiency is low, the product price is relatively expensive, and can only be used to treat ammonia nitrogen wastewater with low concentration such as lower than 500mg / L, such as the nitrification disclosed in Chinese patent CN1509991A The bacterial agent is mainly used for the removal of ammonia nitrogen in seawater aquaculture and high-density aquaculture in industrialized circulating seawater

Chinese patent CN200810228404.0 discloses a production method for treating ammonia-containing wastewater microbial flora, which adopts three-stage amplified cultivation, the first-stage adopts the method of adding ammonium salt in batches, and the second-stage and third-stage cultivation adopts batch supplementation. Alternate material and batch water changes. Although this method realizes the scale-up cultivation of the bacterial flora, it can realize the industrialization and commercialization of the ammonia nitrogen-removing bacterial flora, but the number of cultivation stages and the cultivation time of the flora are relatively long.

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