Fermentation method for promoting high yield of oil of rhizopus arrhizus and used culture medium

Abstract

The invention discloses a fermentation method for promoting high yield of oil of rhizopus arrhizus. The method is characterized by firstly regulating and controlling the dissolved oxygen amount in a process of fermentation culture in a segmented way, wherein the higher dissolved oxygen amount at the early stage of fermentation is beneficial to the biomass accumulation of the rhizopus arrhizus, andthe lowering of the dissolved oxygen amount at the later stage of fermentation is beneficial to oil accumulation; secondly, in the stage of oil accumulation at the later stage of fermentation, addingsodium tungstate to promote oil synthesis in thallus of the rhizopus arrhizus to improve oil production. A formula of a fermentation culture medium involved in the invention comprises corn starch, glucose, ammonium nitrate, peanut meal zymolyte, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate, various trace elements, absolute methanol, melatonin, fulvic acid, 2-mercaptoethanol,4-chlorophenoxyacetic acid, N-methylmorpholine and sophorolipid, and the use of the culture medium has an obvious promoting effect on the improvement of the biomass and oil production of the rhizopusarrhizus. The method disclosed by the invention has the advantages that the operation is relatively simple, the production cost can be reduced, and the method is easy to apply to industrial production.

Description

technical field [0001] The invention relates to a fermentation method for promoting the high-yield oil and fat of Rhizopus arrhizus and a culture medium used, belonging to the technical field of microbial fermentation. Background technique [0002] In recent years, the excessive consumption of energy and the use of fossil fuels have brought about a series of environmental problems, and the development of new, environmentally friendly non-petroleum energy and clean and renewable energy is imminent. Due to its unique advantages, biodiesel, as a potential energy alternative to fossil fuels, has received more and more attention in its development and research. At present, the main raw materials used to produce biodiesel at home and abroad are still animal and vegetable oils, but their costs are relatively high. Cheap waste oil has a wide range of sources, but the production utilization rate is low, and the oil composition is relatively complex, and it is difficult to form large...

AI Technical Summary

Problems solved by technology

If the culture time is short, the total number of strains will decrease, and the oil production will decrease accordingly; if the culture time is too long, the microorganisms will have distortion, swelling and other undesirable phenomena, which will make it difficult to collect oil and affect the quality and yield of microbial oil

[0006] The research team found that in the prior art, most of the molds used to ferment and produce oil were Mortierella or Mucor, while there were few studies on the fermentation of oil with Rhizopus arrhizus.

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