Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Formula of improved acetobacter growth and metabolism medium

A technology of medium and acetic acid bacteria, applied in the direction of bacteria, etc., can solve the problems of slow growth, unreasonable medium configuration, and influence on fermentation production, and achieve good fermentation production, rapid activation of growth, and promotion of bacterial growth.

Inactive Publication Date: 2017-08-18
JIANGSU HENGSHUN VINEGAR IND
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above-mentioned defects existing in the prior art field, the present invention aims to provide a kind of improved acetic acid bacteria growth fermentation medium, solves the prior art because the existing configuration of the medium is not reasonable enough, causes the growth speed of acetic acid bacteria to slow down after concentration, thereby affecting Problems with fermentation production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The present embodiment proposes a kind of improved culture medium formula of acetic acid bacteria, and its component ratio by weight is:

[0020] 8 parts of beef extract, 3 parts of glucose, 9 parts of tryptone, 5 parts of sodium chloride, 0.5 part of magnesium sulfate, 0.2 part of calcium lactate, 950 parts of distilled water, pH 7.3.

[0021] After being prepared according to the above formula, the medium was autoclaved at 121.3° C. for 30 minutes, and after the sterilization was completed, it was dried for later use to obtain an improved medium.

[0022] Acetic acid bacteria culture steps are:

[0023] (1) Return the bacteria from the -80°C refrigerated state to room temperature, and thaw the bacteria.

[0024] (2) Aseptic operation Inoculate the thawed bacteria into the culture medium to activate and restore the original vitality of the bacteria.

[0025] (3) Insert the activated acetic acid bacteria species into the improved medium according to the ratio of 5%-8%...

Embodiment 2

[0028] The present embodiment proposes a kind of improved culture medium formula of acetic acid bacteria, and its component ratio by weight is:

[0029] 5 parts of beef extract, 5 parts of glucose, 8 parts of tryptone, 6 parts of sodium chloride, 0.3 parts of magnesium sulfate, 0.3 parts of calcium lactate, 900 parts of distilled water, pH 7.2.

[0030] After being prepared according to the above formula, the medium was autoclaved at 121.3° C. for 30 minutes, and after the sterilization was completed, it was dried for later use to obtain an improved medium.

[0031] Acetic acid bacteria culture steps are:

[0032] (1) Return the bacteria from the -80°C refrigerated state to room temperature, and thaw the bacteria.

[0033] (2) Aseptic operation Inoculate the thawed bacteria into the culture medium to activate and restore the original vitality of the bacteria.

[0034] (3) Insert the activated acetic acid bacteria species into the improved medium according to the ratio of 5%-...

Embodiment 3

[0037] The present embodiment proposes a kind of improved culture medium formula of acetic acid bacteria, and its component ratio by weight is:

[0038] 10 parts of beef extract, 2 parts of glucose, 12 parts of tryptone, 3 parts of sodium chloride, 0.6 part of magnesium sulfate, 0.1 part of calcium lactate, 1000 parts of distilled water, pH 7.4.

[0039] After being prepared according to the above formula, the medium was autoclaved at 121.3° C. for 30 minutes, and after the sterilization was completed, it was dried for later use to obtain an improved medium.

[0040] Acetic acid bacteria culture steps are:

[0041] (1) Return the bacteria from the -80°C refrigerated state to room temperature, and thaw the bacteria.

[0042] (2) Aseptic operation Inoculate the thawed bacteria into the culture medium to activate and restore the original vitality of the bacteria.

[0043] (3) Insert the activated acetic acid bacteria species into the improved medium according to the ratio of 5%...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a formula of an improved acetobacter growth and metabolism medium. The formula comprises the following components by weight: 5-10 parts of beef extract, 2-5 parts of glucose, 8-12 parts of tryptone, 3-6 parts of sodium chloride, 0.3-0.6 part of magnesium sulfate, 0.1-0.3 part of calcium lactate, and 900-1000 parts of distilled water. And the pH of the medium is 7.2-7.4. The formula has effective and reasonable design, can effectively promote acetobacter growth and efficient secretion of metabolic product, and can promote fermentation production.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a medium formula for fermentation and cultivation of acetic acid bacteria. Background technique [0002] After the strain is transferred from the preservation state to the appropriate medium for activation, it is then transferred to the medium for fermentation. The following processes are required from the activation of the strain to the fermentation culture: [0003] (1) prepare the medium for the growth of acetic acid bacteria; [0004] (2) Return the strains from the cold storage state at -80°C to the room temperature state, and thaw the strains; [0005] (3) Aseptically inoculate the thawed strains into the previously prepared culture medium to activate and restore the original vitality of the strains; [0006] (4) Transfer the activated bacterial solution in step (3) to the fresh culture medium prepared in step (1) for fermentation. [0007] The formula o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 姚清余永建朱胜虎李信张俊红
Owner JIANGSU HENGSHUN VINEGAR IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com