Lactobacillus fermentum and application thereof in improving intestinal health

A Lactobacillus fermentum and intestinal health technology, applied in the field of microorganisms, can solve the problems of the lack of pertinence of Parabacteroides, hinder the treatment of Parabacteroides infection, etc., and achieve the effect of improving intestinal health, broad-spectrum oligosaccharide utilization, and huge application prospects.

Inactive Publication Date: 2020-04-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned probiotics lack specificity to Parabacteroides. At present, there is still no probiotic strain that c

Method used

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  • Lactobacillus fermentum and application thereof in improving intestinal health
  • Lactobacillus fermentum and application thereof in improving intestinal health
  • Lactobacillus fermentum and application thereof in improving intestinal health

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Screening and strain identification of Lactobacillus fermentum

[0048] 1. Screening

[0049] Taking the feces of villagers from Luoyi Village, Laocheng Town, Chengmai County, Hainan Province as samples, they were serially diluted 10 times with sterile normal saline to 10 -6 , and then take 100 μL dilution gradient of 10 -4 、10 -5 、10 -6 Spread the diluted liquid on the MRS solid medium, culture at 37°C for 48 hours, observe and record the colony shape; pick the colonies of different shapes on the MRS solid medium to separate them by streaking, and after culturing at 37°C for 48 hours, pick again Take the single colonies of different shapes on the MRS solid medium and separate them by streaking until the pure single colonies with the same shape are obtained; pick the pure colonies on the MRS solid medium and inoculate them in 5mL of MRS liquid medium, and culture them at 37°C for 18h; Put 1mL of the bacterial solution in a sterile centrifuge tube, centrif...

Embodiment 2

[0052] Embodiment 2: the cultivation of lactobacillus fermentum

[0053] Lactobacillus fermentum (Lactobacillus fermentum) CCFM1037 was inoculated on MRS solid medium and cultured at 37°C for 48 hours. The colonies were observed and found to be round, white, transparent and smooth.

Embodiment 3

[0054] Example 3: Tolerance of different lactobacillus fermentum to simulated gastrointestinal fluid

[0055] Specific steps are as follows:

[0056] 1. Tolerance of different Lactobacillus fermentum to simulated gastric juice

[0057] Lactobacillus fermentum (Lactobacillus fermentum) CCFM1037, Lactobacillus fermentum (Lactobacillus fermentum) LF1, Lactobacillus fermentum LF2, Lactobacillus fermentum LF3, Lactobacillus fermentum LF4 obtained in Example 1 , Lactobacillus fermentum LF5, Lactobacillus fermentum LF6, Lactobacillus fermentum LF7, Lactobacillus fermentum LF8, Lactobacillus fermentum LF9 and Lactobacillus fermentum ( Lactobacillus fermentum) LF10 were placed in MRS liquid medium and cultured at 37°C for 18 hours, and the cells were collected by centrifugation. The collected cells were washed with normal saline. After washing, the cells were collected by centrifugation again, and the collected cells were resuspended in pH 3 (pH adjusted by HCl) containing 3g / L pepsi...

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Abstract

The invention discloses lactobacillus fermentum and an application thereof in improving intestinal health, which belongs to the technical field of microorganisms and medicines. The lactobacillus fermentum CCFM1037 screened by the invention can improve the intestinal health, which is specifically embodied in that (1) the lactobacillus fermentum CCFM1037 can grow by taking fructo-oligosaccharide, xylooligosaccharide and galactooligosaccharide as a unique carbon source and metabolize to produce acid, and has broad-spectrum oligosaccharide utilization capability; (2) various short-chain fatty acids such as acetic acid, propionic acid and butyric acid can be produced through in-vitro metabolism; and (3) the relative abundance of Parabacteroides in intestinal tracts can be obviously reduced, sothat the lactobacillus fermentum CCFM1037 has a huge application prospect in the preparation of products (such as foods, medicines or health care products) for improving the intestinal health.

Description

technical field [0001] The invention relates to a strain of lactobacillus fermentum and its application in improving intestinal health, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] The intestinal microecology is the most important and complex ecological environment of the human body, and the intestinal flora is one of the most active components of the intestinal microecology. There are 500 to 1,000 types of microorganisms in the human intestinal tract, among which Firmicutes, Bacteroidetes, Actinomycetes, Proteobacteria and Verrucobacteria are the main part of the normal human intestinal flora structure. [0003] The structure of the gut microbiota is a co-selection of microorganisms and their hosts, and the two interact to maintain the functional stability of the gut microecosystem. Generally speaking, the intestinal flora of a healthy host is in a state of dynamic balance. If the balance is broken du...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/00A23L33/135C12R1/225
CPCA61P1/00A23L33/135A61K2035/115A23V2002/00C12R2001/225C12N1/205A23V2400/143A23V2200/3204
Inventor 田丰伟陈卫翟齐啸陆文伟吉梦馨沈旭丹于雷雷赵建新张灏
Owner JIANGNAN UNIV
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