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Lactobacillus paracasei Lc19 and microbial preparation containing lactobacillus paracasei Lc19

A microbial preparation, Lactobacillus technology, applied in the direction of microorganisms, microorganism-based methods, medical preparations containing active ingredients, etc. Simple treatment, richness-enhancing effects

Active Publication Date: 2021-08-03
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome the problem in the prior art that Lactobacillus paracasei cannot target the main genus related to the disease in the intestinal tract, which leads to its low recovery ability for the diversity of intestinal flora, thereby Provide a kind of Lactobacillus paracasei Lc19 and the microbial preparation comprising it

Method used

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  • Lactobacillus paracasei Lc19 and microbial preparation containing lactobacillus paracasei Lc19

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Embodiment 1

[0058] The present embodiment provides a kind of preparation method of microbial preparation, specifically comprises the following steps:

[0059] Inoculate Lactobacillus paracasei (Lactobacillus paracasei) Lc19 (preservation number is CGMCC NO.17827) into the MRS medium, the inoculum size is 1%, and culture it statically at 37°C for 16 hours, cultivate it to the logarithmic phase, and the number of viable bacteria reaches 10 9 cfu / mL, centrifuge at 8000rpm, collect the wet cells, add the above-mentioned cell protection agent solution, the cell protection agent solution is 5 times the mass of the wet cells to obtain concentrated bacterial liquid, and concentrate the bacterial liquid at 70 Vacuum drying at ℃ to obtain Lc19 inactivated bacteria powder, wherein the number of bacteria in Lc19 inactivated bacteria powder is 2×10 11 pcs / g.

Embodiment 2

[0061] The present embodiment provides a kind of preparation method of microbial preparation, specifically comprises the following steps:

[0062] Inoculate Lactobacillus paracasei (Lactobacillus paracasei) Lc19 (preservation number is CGMCC NO.17827) into the MRS medium, the inoculum size is 2%, and culture it statically at 37°C for 20 hours, cultivate it to the logarithmic phase, and the number of viable bacteria reaches 10 10 cfu / mL, centrifuge at 8000rpm, collect the wet cells, add the above-mentioned cell protection agent solution, the mass of the cell protection agent solution is 3 times the weight of the wet cell mass, add the sterile cell protection agent solution, The concentrated bacterial solution was obtained, and the concentrated bacterial solution was vacuum-dried at 80° C. to obtain Lc19 inactivated bacterial powder, wherein the number of bacteria in the Lc19 inactivated bacterial powder was 6×10 11 a / g.

Embodiment 3

[0064] This embodiment provides a kind of microbial preparation and preparation method thereof, and the raw material formula of this microbial preparation is as shown in Table 1 below:

[0065] Table 1 Microbial preparation formula

[0066]

[0067] The preparation method comprises the following steps:

[0068] (1) Weigh inulin, skimmed milk powder and pumpkin powder according to the formula, mix them evenly and record them as material A;

[0069] (2) Take by weighing 0.6g of resistant dextrin and the Lactobacillus paracasei Lc19 inactivated bacterium powder of formula quantity and mix and record as B material;

[0070] (3) Weigh stachyose, fructooligosaccharide, raffinose, the remaining part of resistant dextrin and silicon dioxide respectively according to the formula amount, and mix them evenly with material A and material B to obtain the product.

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Abstract

The invention discloses lactobacillus paracasei Lc19 and a microbial preparation containing the lactobacillus paracasei Lc19. The lactobacillus paracasei Lc19 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the lactobacillus paracasei Lc19 is CGMCC NO.17827; the lactobacillus paracasei Lc19 not only has a remarkable curative effect on constipation and colitis, but also can improve various characteristic intestinal related florae of constipation and colitis in a targeted manner, improve the abundance of various beneficial bacteria, reduce the abundance of various harmful bacteria, maintain the balance of the intestinal florae, and greatly improve the capability of recovering the health and diversification of the intestinal florae; the lactobacillus paracasei Lc19 also has the advantages of being convenient to eat, simple in treatment, high in remission rate, free of toxic and side effects and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and in particular relates to a Lactobacillus paracasei Lc19 and a microbial preparation containing the same. Background technique [0002] Inflammatory bowel disease (IBD) is a worldwide epidemic, mainly including ulcerative colitis and Crohn's disease. Constipation (constipation) is a symptom of many diseases. The common symptoms are a significant decrease in the frequency of defecation, less than 3 times a week, dry and hard feces, and often accompanied by a feeling of difficulty in defecation (including strenuous defecation, difficulty in defecation, defecation, etc.) functional constipation refers to the primary persistent constipation caused by non-systemic diseases or intestinal diseases, which is related to changes in the law of life, emotional depression, dietary factors, and bowel habits. Intestinal flora disorder and decreased diversity of flora are the main factors lead...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K45/06A61K35/747A61K35/745A61P1/00A61P1/10A61P1/04C12R1/01C12R1/225
CPCC12N1/20A61K45/06A61K35/747A61K35/745A61P1/00A61P1/10A61P1/04A61K2300/00Y02A50/30
Inventor 陈建国吴秀英李周勇姜云芸滕国新
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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