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Purpose of ganoderan in preparation of health food for regulating flora in intestinal tract

A technology for regulating intestinal flora and Ganoderma lucidum polysaccharides, applied in the biological field, can solve problems such as no metagenomic research reports on Ganoderma lucidum polysaccharides

Inactive Publication Date: 2017-05-10
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The various pharmacological activities of Ganoderma lucidum are mostly related to Ganoderma lucidum polysaccharides, but there is no metagenomic study report on which beneficial and harmful bacteria can be regulated by Ganoderma lucidum polysaccharides in the intestinal flora

Method used

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  • Purpose of ganoderan in preparation of health food for regulating flora in intestinal tract
  • Purpose of ganoderan in preparation of health food for regulating flora in intestinal tract
  • Purpose of ganoderan in preparation of health food for regulating flora in intestinal tract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The Ganoderma lucidum polysaccharide of the present invention is prepared by the following preparation method: After crushing the Ganoderma lucidum, add water according to the ratio of Ganoderma lucidum: water = 1g: 5mL, heat and stir at 75°C for 3 hours, and filter the obtained filter residue according to the above-mentioned material-liquid ratio and temperature Continue to extract twice with time, combine three extractions, concentrate under reduced pressure and centrifuge to obtain a clarified concentrate, add 9 times the volume of 95% ethanol to the clarified concentrate according to the volume ratio of the clarified concentrate: 95% ethanol=1:9 After mixing, let stand overnight at 4°C to precipitate, centrifuge to collect the precipitate, and vacuum freeze-dry to obtain Ganoderma lucidum polysaccharide.

Embodiment 2

[0047] The applicant of the present invention has carried out a pharmacological experiment using the Ganoderma lucidum polysaccharide prepared in the above-mentioned Example 1,

[0048] The experimental results are as follows:

[0049] 1. Experimental materials

[0050] 1.1. Experimental Instruments

[0051] Thermo full-wavelength microplate reader: Thermo Fisher Scientific China, 1510;

[0052] JA1203N analytical balance: product of Shanghai Precision Instrument Co., Ltd.;

[0053] Electronic balance: Changshu Shuangjie Testing Instrument Factory, T1000.

[0054] 1.2. Experimental reagents

[0055] Glucose determination kit: Shanghai Rongsheng Biopharmaceutical Co., Ltd., 20131207147;

[0056] Total cholesterol assay kit: Shanghai Rongsheng Biopharmaceutical Co., Ltd., 20131105146;

[0057] Triglyceride determination kit: Shanghai Rongsheng Biopharmaceutical Co., Ltd., 20131105121;

[0058] Low-density lipoprotein assay kit: Shanghai Rongsheng Biopharmaceutical Co., Lt...

Embodiment 3

[0104] The fresh feces of the mice in the 12th week of the mouse experiment in Application Example 1 were collected, and the intestinal flora among different groups were analyzed for differences. figure 1 And B, positive drug group in table 6, C, normal group, D, Ganoderma polysaccharide group 1, E, Ganoderma polysaccharide group 2, F, Ganoderma polysaccharide group 3, M, model group (grouping is the same as table 2 in embodiment 2) .

[0105] This research is most concerned about whether the bacteria with differences between the M model group and the C normal group can be adjusted after treatment, and the figure 1 . figure 1 The OTUs selected in are the OTUs with differences between the C normal group and the M model group (their effectsize>1.96), and then these OTUs are also tested between other groups and the M model group, and the obtained effectsize draws the above heat map . From figure 1 It can be easily seen that the effect of the positive drug is very significant,...

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Abstract

The invention relates to the technical field of biology, and especially relates to a purpose of ganoderan in preparation of health food for regulating flora in intestinal tract. The invention provides the purpose of ganoderan in preparation of health food capable of increasing beneficial bacteria abundance in flora of intestinal tract and reducing harmful bacterium abundance. The applicant of the invention adopts high-fat feed to induce a mouse obesity type-II diabetes model to observe the influence of ganoderan on lipid metabolism of mice through enteric florae, and by adopting the ganoderan, enteric flora disturbance caused by diet can be adjusted, constitution of enteric florae can be remarkably improved, and the unbalance enteric florae in bodies can be recovered, so that the blood fat and the blood sugar indexes of obesity caused by enteric flora disturbance caused by diet can be adjusted, the content of triglyceride in blood plasma can be remarkably reduced, and the content of cholesterol, low density lipoprotein and free fatty acid can be reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use of Ganoderma lucidum polysaccharide in the preparation of health food with the effect of regulating intestinal flora. Background technique [0002] People's eating habits mostly prefer high-fat, high-sugar and high-calorie diets. With the improvement of material living standards, people's demand for high-fat diets is also increasing. A high-fat diet is also one of the important causes of diseases such as obesity and diabetes. Studies in recent years have found that some polysaccharide-rich foods and medicines have better effects in reducing blood sugar and lipids. Studies have shown that gut flora can control fat metabolism, trigger systemic chronic low-grade inflammation, and induce obesity and insulin resistance. Diet structure is an important factor in determining the composition of intestinal flora. Maintaining intestinal microecological balance is necessary to maintai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125
CPCA23V2002/00A23V2250/50A23V2200/32A23V2200/328A23V2200/3262A23V2200/3324
Inventor 胡明华马方励王园园马忠华葛亚中金鑫
Owner INFINITUS (CHINA) CO LTD
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