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Cordyceps militaris intracellular polysaccharide and preparation method thereof, and applications in regulation of intestinal flora

A kind of intracellular polysaccharide, Cordyceps militaris mycelium technology, applied in biological field

Active Publication Date: 2020-04-21
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reports at home and abroad whether the polysaccharide of Cordyceps militaris can play a role in regulating dyslipidemia by regulating the structure of intestinal flora, especially the report on regulating the composition and structure of intestinal fungi and health

Method used

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  • Cordyceps militaris intracellular polysaccharide and preparation method thereof, and applications in regulation of intestinal flora
  • Cordyceps militaris intracellular polysaccharide and preparation method thereof, and applications in regulation of intestinal flora
  • Cordyceps militaris intracellular polysaccharide and preparation method thereof, and applications in regulation of intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1: Preparation of Cordyceps militaris intracellular polysaccharide

[0104] Cordyceps militaris is activated by strain, cultured on inclined plane and liquid submerged fermentation to obtain Cordyceps militaris mycelium, and the concrete method is as follows:

[0105] 1. Cultivation of slanted surface strains: after the activation and cultivation of the slanted parent species of Cordyceps militaris, it is inoculated into a sterilized slanted solid medium, and cultivated at 24 ° C for 8 d, for subsequent use;

[0106] ②Liquid submerged fermentation culture: Pick 4 to 6 soybean-sized bacterial blocks from the slanted strains of Cordyceps militaris, inoculate them in 150 mL of aseptic liquid fermentation medium, and conduct constant-temperature shaking culture in the dark at 26°C and 160 rpm in the dark. 7d, obtain the Cordyceps militaris liquid fermentation product, and separate through centrifugation and vacuum filtration to obtain the Cordyceps militaris myceliu...

Embodiment 2

[0122] Example 2: Preparation of Cordyceps militaris intracellular polysaccharide

[0123] (1) Cordyceps militaris is activated by bacterial classification, slanted surface bacterial culture and liquid submerged fermentation are cultivated to obtain Cordyceps militaris mycelium, and the concrete method is as follows:

[0124] 1. Cultivation of slanted surface strains: after the activation and cultivation of the slanted parent species of Cordyceps militaris, it is inoculated into a sterilized slanted solid medium, and cultivated at 24 ° C for 8 d, for subsequent use;

[0125] ②Liquid submerged fermentation culture: Pick 4 to 6 soybean-sized bacterial blocks from the slanted strains of Cordyceps militaris, inoculate them in 150 mL of aseptic liquid fermentation medium, and conduct constant-temperature shaking culture in the dark at 26°C and 160 rpm in the dark. 7d, obtain the Cordyceps militaris liquid fermentation product, and separate through centrifugation and vacuum filtrati...

Embodiment 3

[0141] Example 3: Preparation of Cordyceps militaris intracellular polysaccharide

[0142] (1) Cordyceps militaris is activated by bacterial classification, slanted surface bacterial culture and liquid submerged fermentation are cultivated to obtain Cordyceps militaris mycelium, and the concrete method is as follows:

[0143] 1. Cultivation of slanted surface strains: after the activation and cultivation of the slanted parent species of Cordyceps militaris, it is inoculated into a sterilized slanted solid medium, and cultivated at 24 ° C for 8 d, for subsequent use;

[0144] ②Liquid submerged fermentation culture: Pick 4 to 6 soybean-sized bacterial blocks from the slanted strains of Cordyceps militaris, inoculate them in 200 mL of sterile liquid fermentation medium, and culture under constant temperature shaking in the dark at 26°C and 160 rpm in the dark 7d, the Cordyceps militaris liquid fermentation product is obtained, and the Cordyceps militaris mycelium is obtained thro...

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Abstract

The invention discloses cordyceps militaris intracellular polysaccharide and a preparation method thereof, and applications of the cordyceps militaris intracellular polysaccharide in regulation of intestinal flora. The method comprises the following steps: carrying out fermentation culture on cordyceps militaris strains, and separating to obtain cordyceps militaris mycelia; degreasing the cordyceps militaris mycelia, and performing hot water leaching to obtain a cordyceps militaris leaching liquid; and removing protein by adopting trypsin and a Sevage method, carrying out ethanol precipitation, and carrying out DEAE-Sepharose Fast Flow column chromatography purification to obtain the cordyceps militaris intracellular polysaccharide. The cordyceps militaris intracellular polysaccharide prepared by the invention can regulate the intestinal bacterial flora structure of a high-fat diet mouse, promote the growth of intestinal probiotics of high-fat diet mice, improve the proportion of phylum firmicutes / bacteroides, adjust the structure of fungal flora, promote the synthetic route of intestinal butyrate, induce activation of short-chain fatty acid receptors, promote fat metabolism and inhibit fat accumulation so as to achieve the effects of reducing blood fat and protecting the liver.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an intracellular polysaccharide of Cordyceps militaris, a preparation method and its application in regulating intestinal flora. Background technique [0002] Intestinal flora, the normal microorganisms that live in the human intestine, its number is more than 10 times that of human cells. Under normal circumstances, there is a dynamic balance between the intestinal flora and the host, and between the intestinal flora, the intestinal environment and the host. The gut microbiota are interdependent and restrict each other, and together they form a relatively stable gut microecological environment. Intestinal flora is the largest immune organ in the human body. It plays an important role in regulating the immune system, endocrine system and nervous system of the human body, and mediates the metabolic pathways of various endocrine diseases. [0003] A large number of studie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A01G18/00A01G18/20A01G18/40A23L31/00A23L33/125A61K31/715A61P1/00A61P3/06
CPCC08B37/0003A61K31/715A61P1/00A61P3/06A23L31/00A23L33/125A01G18/00A01G18/20A01G18/40A61K36/068A23V2002/00A23V2200/32A23V2250/208A23V2250/51A23V2300/14
Inventor 张松黄振峰
Owner SOUTH CHINA NORMAL UNIVERSITY
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