Application of dimethoxyphenol and biological preparation thereof in prevention and treatment of plant pathogenic nematodes

By combining the application of *Polyspora pinkis* and *Bacillus belysinus*, 2,3-dimethoxyphenol and isopentenyl adenine were identified as key substances, and a compound biological agent with both nematicidal and growth-promoting properties was prepared. This solved the problem of the single function of existing microbial pesticides and achieved efficient and stable disease control and plant growth promotion effects.

CN122250460APending Publication Date: 2026-06-23INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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Patent Information

Application Number
CN202610205688.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-12
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

Existing microbial pesticides and growth promoters have limited functions, unclear active substances, vague mechanisms of action, and uncontrollable processes, resulting in unstable product efficacy and difficulty in achieving the combined functions of disease control and plant growth promotion.

Method used

By combining the application of Clonostachys rosea NF-06 and Bacillus velezensis YB-1652, metabolomics analysis revealed that 2,3-dimethoxyphenol and isopentenyl adenine are the key substances for nematicidal and life-promoting effects, and a compound biological agent with both nematicidal and life-promoting properties was prepared.

Benefits of technology

This has resulted in a biological agent with a clear mechanism and well-defined components, which significantly enhances the synergistic effect of nematicide control and plant growth promotion. The process is stable and controllable, environmentally friendly, and highly safe for application, making it suitable for the needs of green agriculture.

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Abstract

The invention discloses application of dimethoxyphenol and a biological agent thereof in prevention and treatment of plant pathogenic nematodes, and aims to solve the problems that an existing microbial source preparation is single in function, unknown in active ingredient, unclear in mechanism and unstable in quality. According to the invention, 2, 3-dimethoxyphenol is accurately identified as an important active substance for killing nematodes through metabonomics, and stable co-accumulation of 2, 3-dimethoxyphenol and a growth promoting factor isopentenyl adenine is realized in combination with a co-culture process of specific strains (spiropolyspora roseum NF-06 and bacillus velezensis YB-1652); and the technical effects of definite components, synergism of disease prevention and growth promotion functions, controllable quality and environmental friendliness are achieved.
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