The invention discloses a preparation method of an
apple pectin heteropolysaccharide for improving diversity of intestinal florae. The preparation method comprises the following steps: I, dissolving apple
powder into water, performing enzymolysis, performing vacuum concentration, and performing purification so as to obtain a
pectin extract; II, adding
pectin methylesterase and
pectin acetylase into the pectin extract, performing primary oriented
digestion, adding polygalacturonase and
pectate lyase, performing secondary oriented
digestion, further adding polyrhamnogalacturonases, xylogalact uronase,
galactan esterases and arabanase, and performing third oriented
digestion so as to obtain a modified pectin extract; and III, performing purification treatment on the modified pectin extract, so as to obtain the
apple pectin heteropolysaccharide. Through three times of oriented digestion, the
apple pectin heteropolysaccharide which is high in neutral
polysaccharide ratio, low in methyl esterification degree and low in
acetylation degree can be obtained, the apple pectin heteropolysaccharide is capable effectively improving diversity of intestinal florae, and the
microorganism fermentation degradation rate in intestines within 24 hours is greater than 80%.