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Method for preparing pectin oligosaccharide (POS) through dynamic high-pressure microfluidization (DHPM) combined acid process

A technology of high-pressure micro-jet and pectin oligosaccharides, which is applied in the preparation of oligosaccharides and the field of beneficial activities of oligosaccharides, can solve the problems of low yield of oligomers, high ash content, and many colored substances, and achieve Good probiotic activity, short reaction time, environment-friendly effect

Inactive Publication Date: 2012-08-01
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Degrading pectin with inorganic acid is the earliest method of degrading pectin, but mild hydrolysis conditions cannot break the pectin mainly composed of uronic acid, and strong acid and prolonged hydrolysis time may cause side reactions. High, the more side reactions, the more colored substances are produced, the color is dark, and the amount of alkali required to neutralize is large, so the ash produced is also more, the hydrolysis reaction is not easy to control, and the yield of oligomers is not high
Enzyme degradation is a method of degrading pectin by specific or non-specific pectinase, which requires a long operating time and is sensitive to pH changes. Adding enzymes makes industrial production more difficult
As for the physical degradation method, only the preparation of pectin oligosaccharides by radiation has been reported.

Method used

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  • Method for preparing pectin oligosaccharide (POS) through dynamic high-pressure microfluidization (DHPM) combined acid process
  • Method for preparing pectin oligosaccharide (POS) through dynamic high-pressure microfluidization (DHPM) combined acid process

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Embodiment 1

[0020] Weigh 18.4g pectin and dissolve it in 1000ml distilled water, stir at room temperature until completely dissolved, and use 0.5MH 2 SO 4 Adjust the pH of the solution to H in the pectin solution 2 SO 4 The concentration is 0.15 M, and the water bath is heated to 63 o c. Add the heated pectin solution into the micro jet, and treat it under 160MPa for 6 times to get the degraded pectin solution. Add 10% pectin solution calcium carbonate powder to the pectin solution, stir well, centrifuge, and take the supernatant. The supernatant was ultrafiltered for 6 h in a tangential flow ultrafilter with a molecular weight cut off of 5,000 Da, and the permeate was collected and concentrated. Put the permeate at -80 o Freeze-drying under C conditions for 48 hours to obtain pectin oligosaccharides. Inoculate Bifidobacterium, Lactobacillus, and Escherichia coli in 10 ml minimal medium with 1% (w / v) glucose as carbon source, 37 o C Cultivate for 24 hours under anaerobic condit...

Embodiment 2

[0022] Weigh 36.8 g pectin and dissolve it in 1000 ml distilled water, stir at room temperature until completely dissolved, and use 0.5MH 2 SO 4 Adjust the pH of the solution to H in the pectin solution 2 SO 4 The concentration is 0.15 M, and the water bath is heated to 63 o c. Add the heated pectin solution into the micro jet, and treat it under 160MPa for 6 times to get the degraded pectin solution. Add 10% pectin solution calcium carbonate powder to the pectin solution, stir well, centrifuge, and take the supernatant. The supernatant was ultrafiltered for 6 h in a tangential flow ultrafilter with a molecular weight cut off of 5,000 Da, and the permeate was collected and concentrated. Put the permeate at -80 o Freeze-drying under C conditions for 48 hours to obtain pectin oligosaccharides. Inoculate Bifidobacterium, Lactobacillus, and Escherichia coli in 10 ml minimal medium with 1% (w / v) glucose as carbon source, 37 o C Cultivate for 24 hours under anaerobic cond...

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Abstract

A preparation method of POS is provided. The POS is prepared through treating a commercial apple pectin as a raw material, adopting a DHPM combined acid hydrolysis process, and carrying out dissolving, acid adding, DHPM processing, neutralizing, ultrafiltering and the like. The method which adopts the DHPM combined acid hydrolysis process to degrade the pectin makes the oligosaccharide having the activity beneficial for lives be obtained, so the method has the characteristics of rapid reaction speed, high yield, environmental protection and the like.

Description

[0001] technical field [0002] The invention relates to a method for preparing pectin oligosaccharides by dynamic high-pressure micro-jet combined with an acid method, and relates to a preparation method of oligosaccharides and the probiotic activity of oligosaccharides. Background technique [0003] Oligosaccharides, also known as oligosaccharides, generally refer to glycosides composed of 2-20 monosaccharides. Different types and sources of oligosaccharides determine their unique biological activities. In general, oligosaccharides have the following biological activities: gastrointestinal tract activity, hematopoietic function enhancement activity, dental caries prevention, blood sugar stabilization and immune activity improvement. [0004] Pectin is a general term for a series of oligosaccharides and polysaccharides with the same properties but different fine structures. It is one of the polysaccharides that make up the cell wall of plants. It is widely present in the ...

Claims

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Application Information

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IPC IPC(8): C08B37/06
Inventor 梁瑞红陈军刘成梅刘伟
Owner NANCHANG UNIV
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