Probiotic active compound polysaccharide as well as preparation method and application thereof

A compound polysaccharide and living technology, applied in the direction of application, polysaccharide/gum-containing food ingredients, food ingredients as odor improvers, etc., can solve the adverse effects of product color and odor, improve intestinal microorganisms without prebiotics, and study probiotics Less problems such as polysaccharide browning degree is not high, facility requirements are not high, and the effect of reducing the dissolution rate

Active Publication Date: 2022-06-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods also have some disadvantages: (1) Although the cell wall structure can be quickly destroyed by physical means such as steam explosion and ultra-high pressure treatment, a variety of supporting equipment is required, and the requirements for mechanical equipment are relatively high; (2) the use of heat The polysaccharide extracted by water or alkali contains more protein, which needs to be further deproteinized, and the process is relatively cumbersome. In addition, high temperature and alkaline pH conditions will accelerate the Maillard reaction, which may have adverse effects on the color and smell of the product; (3) Purification Most of the process uses organic reagents to remove impurities, such as acetone degreasing and Sevag reagent deproteinization, which is difficult to apply to food processing enterprises
Not only that, there are few studies on the probiotic activity of a single Lycium barbarum polysaccharide or laminar polysaccharide, and there is no report on the combination of Lycium barbarum polysaccharide and laminar polysaccharide as a prebiotic to improve intestinal microorganisms

Method used

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  • Probiotic active compound polysaccharide as well as preparation method and application thereof
  • Probiotic active compound polysaccharide as well as preparation method and application thereof
  • Probiotic active compound polysaccharide as well as preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0063] (1) Preparation of standard products: equal volume of 8 monosaccharide standard products (10 mg / mL, solvent For ultrapure water) 20μL in a stoppered test tube, mix well, as a mixed standard. Pipette 50μL of mixed standard into a stoppered test tube, add 50μL of 0.6mol / L NaOH aqueous solution, and 100μL of 0.5mol / L 1-phenyl-3-methyl-5-pyrazolone (PMP) methanol solution. The derivatization reaction was carried out at 70 °C for 100 min and cooled. Add 100 μL of 0.3 mol / L HCl solution, make up with water until the final volume is 2 mL, add 2 mL of chloroform, mix well, centrifuge at 2000 rpm for 5 min, discard the chloroform phase, repeat the extraction 6 times, take the aqueous phase through a 0.22 μm microporous membrane, and wait for HPLC sample analysis.

[0064] (2) Sample preparation: Take 1 mL of 10 mg / mL sample solution into an ampoule bottle, add 1 mL of 4 mol / L trifluoroacetic acid aqueous solution, seal, and hydrolyze at 110 °C for 8 h; take out and cool, add 4...

Embodiment 1

[0070] A preparation method of probiotic composite polysaccharide, specifically comprising the following steps:

[0071] The extraction conditions of Lycium barbarum polysaccharide for compounding are as follows:

[0072] (1) Lycium barbarum homogenate to remove impurities: Weigh 60g of dry wolfberry, add 70% (v / v) ethanol aqueous solution according to the ratio of material to liquid 1:6g / mL, pass through colloid mill twice, at a stirring speed of 120r / min and a temperature of 80 Under the condition of ℃, reflux extraction for 1h, centrifuge under the conditions of centrifugal force of 6000g and centrifugation time of 15min, discard the supernatant, recover the residue, repeat the extraction according to the above steps, the drying temperature of the recovered residue is 50℃, and the drying time is 10h , get residue A1;

[0073] (2) Enzymatic hydrolysis: 18 g of residue A1 was added to deionized water at a ratio of 1:20 g / mL of material to liquid, and 0.6% by mass of residue ...

Embodiment 2

[0094] A preparation method of probiotic composite polysaccharide, specifically comprising the following steps:

[0095] The extraction conditions of Lycium barbarum polysaccharide for compounding are as follows:

[0096] (1) Lycium barbarum homogenate to remove impurities: weigh 60g of dry wolfberry, add 80% (v / v) ethanol aqueous solution according to the material-to-liquid ratio of 1:8 g / mL, and pass through colloid mill 3 times at a stirring speed of 150r / min and a temperature of 85 Under the condition of ℃, reflux extraction for 1.5h, centrifuge under the conditions of centrifugal force of 7000g and centrifugation time of 20min, discard the supernatant, recover the residue, repeat the extraction twice according to the above steps, the drying temperature of the recovered residue is 55℃, and the drying time 11h, get residue B1;

[0097] (2) Enzymatic hydrolysis: 18 g of residue B1 was added to deionized water at a ratio of 1:30 g / mL of material to liquid, and at the same ti...

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PUM

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Abstract

The invention discloses life-benefiting active compound polysaccharide as well as a preparation method and application thereof. The compound polysaccharide is a composition of lycium barbarum polysaccharide and laminarin. The lycium barbarum polysaccharide and the laminarin are respectively prepared by the processes of crushing, impurity removal, pectinase and cellulase enzymolysis, medium-temperature acidic pH extraction, centrifugation, concentration, alcohol precipitation, redissolution, dialysis, concentration and freeze-drying, and then the lycium barbarum polysaccharide and the laminarin are combined according to a certain mass ratio to prepare the compound polysaccharide. The compound polysaccharide obviously promotes the proliferation of four types of intestinal beneficial bacteria (bacteroides, butyric acid producing bacteria, bifidobacterium and lactobacillus), and can be applied to food and health care products.

Description

technical field [0001] The invention belongs to the technical field of intensive and deep processing of wolfberry and kelp, and particularly relates to a probiotic active compound polysaccharide and a preparation method and application thereof. Background technique [0002] The gut microbiota is composed of a wide variety of microorganisms, and humans and the gut microbiota form a complex interaction and a symbiotic relationship with dynamic balance. Generally, disturbances of the microbiota in the human gut are associated with a variety of diseases, including obesity, diabetes, autoimmune regulation, cardiovascular disease, and inflammatory bowel disease. Probiotics are a group of microorganisms that are beneficial to human health. The traditional probiotics are Bifidobacterium and Lactobacillus, which can regulate the diversity and structure of intestinal microbial community, and can also reduce intestinal barrier permeability, regulate intestinal mucosal immune response,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08C08L5/00C08B37/00A23L33/21A23L2/52A23L2/56
CPCC08L5/08C08B37/0024C08B37/0045A23L33/21A23L2/52A23L2/56A23V2002/00C08L5/00A23V2200/3202A23V2250/51A23V2200/15Y02A50/30
Inventor 赵谋明范家琪林恋竹陈倩妮
Owner SOUTH CHINA UNIV OF TECH
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