Microbial preparation with inactivated bacteria MN002 as well as preparation method and application of microbial preparation

A microbial preparation and microbial technology, applied in the field of microbial preparations, can solve the problems of increased risk of bacteriophage infection and outbreak, harsh product storage conditions, and susceptibility to antibiotics, etc., to improve the intestinal flora with characteristics of colitis, excellent heat resistance, etc. and pH stability, improving the effect of intestinal flora characteristic of constipation

Active Publication Date: 2021-08-03
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]The above functions are all based on live bacteria, and for probiotic products made of live bacteria, there are many deficiencies as follows that affect the quality of the product Efficacy, including: (1) When the intestinal tract is immature, inflamed or immune insufficiency, live bacteria will break through the intestinal wall and invade the body, thereby causing inflammation; (2) It is susceptible to antibiotics, and it is also easy to cause drug resistance gene transfer; ( 3) Long-term use of live bacteria preparations increases the risk of phage infection and outbreak; (4) Poor processing characteristics such as heat resistance, easy pollution, and product instability; (5) Storage conditions for products such as low temperature and light protection are relatively harsh

Method used

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  • Microbial preparation with inactivated bacteria MN002 as well as preparation method and application of microbial preparation
  • Microbial preparation with inactivated bacteria MN002 as well as preparation method and application of microbial preparation
  • Microbial preparation with inactivated bacteria MN002 as well as preparation method and application of microbial preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]A microbial preparation comprising inactivated Streptococcus thermophilus MN002. The microbial preparation is a microbial preparation with inactivated Streptococcus thermophilus MN002 as the active ingredient, and the treated product is the culture, concentrate, dry matter, liquid, and dilution of Streptococcus thermophilus MN002 and at least one of fragments. The microbial preparation also includes at least one auxiliary agent suitable for microbial preparation, such as: protective agent, excipient, etc.; the dosage form of the microbial preparation is powder, pill, capsule, granule, tablet, liquid preparation or gels.

[0037] The preparation method of above-mentioned microbial preparation among the present invention, comprises:

[0038] (1) The strains of Streptococcus thermophilus MN 002 were activated and cultured with MRS liquid medium, and cultured statically until the logarithmic phase, so that the number of viable bacteria reached 10 9 More than cfu / mL;

[0...

Embodiment 2

[0045] A kind of inactivated bacteria powder, the specific preparation process is as follows:

[0046] (1) The fermented strain Streptococcus thermophilus MN002 was activated and cultured with MRS liquid medium, and cultured statically for 20 hours at 37°C until the logarithmic phase, so that the number of viable bacteria reached 10 9 More than cfu / mL;

[0047] (2) Centrifuge to collect wet thallus, add protective agent, protective agent is skimmed milk powder, lactose, trehalose, inulin, maltodextrin and sodium glutamate, wet thallus adds protective agent (wet Bacteria: protective agent = 7:1), vacuum drying at 70°C to obtain MN 002 inactivated bacteria powder.

Embodiment 3

[0049] A product containing microbial preparations, wherein the inactivated bacteria powder prepared in Example 1 is added, the product can be a medicine, the product can also be a health product, and it can also be a food that can be used to improve the structure of the intestinal flora Wait.

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PUM

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Abstract

The invention discloses a microbial preparation with inactivated streptococcus thermophilus MN002 as well as a preparation method and application of the microbial preparation. The streptococcus thermophilus MN002 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 7, 2010, and the preservation number is CGMCC No.3817. The inactivated streptococcus thermophilus MN002 product disclosed by the invention has the effects of improving colitis and characteristic intestinal flora thereof, and improving constipation and characteristic intestinal flora thereof.

Description

technical field [0001] The present invention relates to microbial preparations and applications with inactivated Streptococcus thermophilus MN002. Background technique [0002] Streptococcus thermophilus (Streptococcus thermophilus) is a facultative anaerobic or slightly aerobic Gram-positive bacterium. Two oval-shaped cocci form a pair of long chains of about 0.7 to 0.9 microns. Streptococcus thermophilus Colonies in selective media are beige. Streptococcus thermophilus belongs to probiotics, and the microorganisms classified as probiotics need to be still active when they reach the intestinal tract. For this reason, these bacteria should be alive when they pass through the gastrointestinal tract. Studies have shown that Streptococcus thermophilus can reach the upper part of the small intestine, but not the large intestine like Bifidobacteria. The functions of Streptococcus thermophilus disclosed in the prior art mainly include the following: [0003] (1) Live Streptococ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/744A61P1/00A61P1/10C12R1/46
CPCC12N1/20A23L33/135A61K35/744A61P1/00A61P1/10A23V2002/00A23V2400/249A23V2200/3204
Inventor 陈建国吴秀英李周勇姜云芸滕国新韩雨婷
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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