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Ochrobactrum anthropi M11 for degrading unsymmetrical dimethylhydrazine and method for degrading unsymmetrical dimethylhydrazine by using ochrobactrum anthropi M11

A technology of Bacillus pallidum and unsymmetrical dimethyl hydrazine, applied in the biological field, can solve the problems of high operating cost, low safety factor and high energy consumption

Inactive Publication Date: 2013-06-05
LOGISTICS DEPT ANTIEPIDEMIC ARMY OF THE GENERAL ARMAMENT DEPT PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These unsymmetrical dimethylhydrazine treatment methods have problems such as high energy consumption, high operating costs, low safety factor, and immature technology.

Method used

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  • Ochrobactrum anthropi M11 for degrading unsymmetrical dimethylhydrazine and method for degrading unsymmetrical dimethylhydrazine by using ochrobactrum anthropi M11

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Experimental program
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Effect test

Embodiment 1

[0015] The present embodiment provides a method for degrading unsymmetrical dimethylhydrazine by using Pallidum pallidum M11, the method comprising the following steps:

[0016] Seed culture: inoculate the strain of Pallidum bacillus M11 on the slant medium, and cultivate it at 29-31°C for 24 hours to obtain the slant culture. The above-mentioned slant medium composition: peptone 6-14g / L, corn steep liquor 1-5g / L L. KNO 3 0.5~5g / L, K 2 HPO 4 0.1~5g / L, MgSO 4 1~5g / L and agar 15g / L. Take the well-grown slant culture and inoculate it into a 250mL Erlenmeyer flask containing 50-102mL of seed medium, and culture it for 12-24 hours under the conditions of the shaker flask rotation speed of 150-250r / min and the culture temperature of 28-37°C. Obtain seed liquid; above-mentioned seed medium composition: peptone 6~14g / L, corn steep liquor 1~5g / L, KNO 3 0.5~5g / L, K 2 HPO 4 0.1~5g / L and MgSO 4 1~5g / L.

[0017] Seed liquid solidification: After the seed liquid is immobilize...

Embodiment 2

[0022] Seed culture: inoculate the slant medium with the strain of Pallidum pallidum M11, and cultivate it at 30°C for 24 hours to obtain the slant culture; the composition of the above slant medium: peptone 6g / L, corn steep liquor 1g / L, KNO 3 3g / L and K 2 HPO 4 1g / L, MgSO 4 5g / L and agar 15g / L. Take the well-grown slant culture and inoculate it into a 250mL Erlenmeyer flask equipped with 90mL of seed medium, and cultivate it for 12 to 24 hours under the conditions of a shaker flask rotation speed of 250r / min and a culture temperature of 35°C to obtain a seed liquid. Medium composition: peptone 6g / L, corn steep liquor 1g / L, KNO 3 3g / L, K 2 HPO 4 0.1g / L, MgSO 4 5g / L.

[0023] Seed liquid solidification: After immobilizing the seed liquid, obtain solidified bacterial balls; weigh 3g of sodium alginate, 3g of calcium carbonate, and 2g of silicon dioxide, mix them evenly, add them to 100mL of distilled water at 80-100°C, heat and keep stirring , until the solution is...

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Abstract

The invention discloses an ochrobactrum anthropi M11 for degrading unsymmetrical dimethylhydrazine and a method for degrading the unsymmetrical dimethylhydrazine by using the ochrobactrum anthropi M11. The method comprises the steps of: inoculating a strain of the ochrobactrum anthropi M11 to a slant culture medium, and culturing to obtain a slant culture product; inoculating the slant culture product into a container in which a seed culture medium is arranged, and culturing to obtain a seed liquid; immobilizing the seed liquid to obtain immobilized mycelial pellets; and inoculating the immobilized mycelial pellets into a container in which a substrate solution is filled according to an inoculation amount of 10-30%, mixing, and degrading for 72 hours at a temperature of 30-37 DEG C and the oscillation rate of 120-200r / min, wherein the inoculation amount is calculated based on the volume proportion of the seed liquid to the substrate solution. According to the invention, the degrading rate for the unsymmetrical dimethylhydrazine is high up to 97.7%.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a Pallidum pallidum M11 degrading unsymmetrical dimethylhydrazine and a method for degrading unsymmetrical dimethylhydrazine. Background technique [0002] Unsymmetrical dimethylhydrazine, the English name is Unsymmetrical Dimethylhydrazine, referred to as UDMH, and the molecular formula is (CH 3 ) 2 NNH 2 , has a strong irritating fishy odor, is flammable, volatile, colorless or slightly yellowish transparent liquid. The molecular weight of unsymmetrical dimethylhydrazine is 60.11, the boiling point is 63°C, the freezing point is -57.2°C, and the density is 0.791g / cm 3 , has good hydrophilicity, is miscible with water, and can be mixed with gasoline, alcohol and other organic solvents. Unsymmetrical dimethylhydrazine is reductive and can be slowly oxidized by air at room temperature. [0003] As a propellant, unsymmetrical dimethylhydrazine has high calorific value, hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/26
Inventor 夏本立王力丛继信张光友范春华曹晔彭清涛
Owner LOGISTICS DEPT ANTIEPIDEMIC ARMY OF THE GENERAL ARMAMENT DEPT PLA
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