A kind of azoxystrobin degrading bacterium and its production bacterium agent and application

A technology for azoxystrobin and preparations, which is applied in the field of azoxystrobin degrading bacteria and the inoculants produced by them, and can solve the problems of not being able to meet the bioremediation of pesticide residue pollution and the like

Active Publication Date: 2018-03-06
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity of pesticide structures, microbial degradation of pesticides is often highly targeted, resulting in the existing resource pool of degrading bacteria far from meeting the actual needs of bioremediation of pesticide residue pollution.
At present, there are no degrading microorganisms and related degradation preparations specifically targeting azoxystrobin

Method used

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  • A kind of azoxystrobin degrading bacterium and its production bacterium agent and application
  • A kind of azoxystrobin degrading bacterium and its production bacterium agent and application
  • A kind of azoxystrobin degrading bacterium and its production bacterium agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of Pallidum pallidus SH14

[0032] The inventors isolated and screened the azoxystrobin-degrading bacteria by enrichment culture method. Environmental samples for enrichment of azoxystrobin were collected from activated sludge from sewage treatment ponds in Singapore. Get activated sludge sample 5g and add 50mL basal medium (formulation is: (NH 4 ) 2 SO 4 , 2g; MgSO4, 0.2g; CaCl 2 , 0.01g; FeSO 4 , 0.005g; MnCl 2 , 0.002g; K 2 HPO 4 , 10.5g; KH 2 PO 4 , 4.5g, in distilled water 1000mL, pH 7.5). At the same time, the mother liquor of azoxystrobin original drug (DMSO as solvent) was added to make the final mass concentration of azoxystrobin 50 mg / L, and the degrading microorganism enrichment culture was carried out at 30° C. and 150 r / min on a shaking table. After culturing for 7 days, they were transferred to the second batch of basal medium containing 100 mg / L azoxystrobin at an inoculum size of 10%. After culturing und...

Embodiment 2

[0038] The preparation of embodiment 2 pale bacillus SH14 liquid preparation

[0039] The production process of using the above-mentioned Pallidum pallidus SH14 to prepare the liquid preparation is as follows: slant seed - shake flask seed solution - seed tank cultivation - production tank fermentation - packaging as liquid bacterial preparation.

[0040] The strains of Paleobacterium pallidum SH14 were activated on LB solid plate, and inoculated on the inclined surface of the test tube for later use. Inoculate the test tube species of Pallidum pallidus SH14 into 1000mL shake flask containing 250mL of LB medium. The formula of LB medium is: 0.5% yeast extract, 1% peptone, 1% NaCl, pH value is 7.5. Constant temperature shaking at 30°C To the logarithmic phase, prepare to inoculate the seed tanks, and the liquid volume of the seed tanks is 70%. 1.1Kg / cm after feeding 3 Under the pressure of 121 ℃, high-pressure damp heat sterilization, after cooling to 30 ℃, inoculate the abov...

Embodiment 3

[0042] The biodegradation effect of embodiment 3 Pallidum SH14 to azoxystrobin

[0043] Add azoxystrobin in basic culture medium (with embodiment 1), make its final concentration be 50mg / L; Inoculation concentration is 1.0 * 10 7 CFU / mL bacterial agent, and set the medium without inoculation as the control, culture at 30°C for 0-5 days, take samples regularly, use HPLC method to measure the residual amount of pesticides and calculate the degradation rate, the results are shown in Table 1 and image 3 shown. The results showed that P.pallidum SH14 could effectively degrade azoxystrobin in a short period of time, the degradation rate of azoxystrobin reached 76.8% within 3 days, and the degradation rate of azoxystrobin reached 86.3% within 5 days, which has a good biodegradation effect .

[0044] Table 1 Degradation effect of Pallidum SH14 on azoxystrobin

[0045]

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Abstract

The invention discloses an azoxystrobin-degrading bacterium as well as the bacterium agent produced therefrom and application thereof. The strain is Ochrobactrum anthropi SH14, which was deposited in China Center for Type Culture Collection (CCTCC) on December 20, 2013, and the preservation number is CCTCC NO: M 2013681. The strain can effectively degrade azoxystrobin pesticide residues in a short period of time, and can be used to restore natural environments such as water bodies and soils polluted by azoxystrobin. The preparation prepared by the bacterial strain has the advantages of low production cost, convenient use, good removal effect and the like. The use of this bacterial agent can reduce the residual amount of azoxystrobin in water and soil by more than 85% in a short period of time, effectively repair the natural environment polluted by azoxystrobin, solve the problem of excessive pesticide residues and environmental pollution in agricultural production, and protect the ecology environment and human health.

Description

technical field [0001] The invention relates to the fields of applied environmental microorganisms and agriculture, in particular to an azoxystrobin-degrading bacterium and the bacterial agent produced by it and its application, which is specifically used to repair natural environments such as water bodies or soil polluted by azoxystrobin, and protect the ecological environment and human health . Background technique [0002] Azoxystrobin, also known as Amida, is one of the most commonly used methoxyacrylate fungicides. Azoxystrobin was launched on the market in 1996 and put into agricultural production applications. It has been registered and used in more than 80 crop diseases in 72 countries around the world. The fungicide has an unprecedented ultra-broad bactericidal spectrum, and has good antibacterial activity against most of the pathogenic fungi in Ascomycetes, Basidiomycetes, Deuteromycetes and Oomycetes, and has been widely used in recent years. It is the main fung...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 陈少华刘诗胤廖立胜程莹莹王惠杉
Owner SOUTH CHINA AGRI UNIV
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