Human ochrobactrum capable of efficiently degrading glyphosate and application of human ochrobactrum

A technology of Bacillus hominis and glyphosate, which is applied to Bacillus hominis hominis for efficiently degrading glyphosate and its application field, and achieves the effect of great application value

Active Publication Date: 2022-07-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of the existing glyphosate residue degradation and restoration technology, and provide a new efficient glyphosate-degrading microbial bacteria, degradation bacterial agent and degradation method, which can be used to quickly and efficiently degrade glyphosate , remediation of soil and water bodies polluted by glyphosate residues

Method used

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  • Human ochrobactrum capable of efficiently degrading glyphosate and application of human ochrobactrum
  • Human ochrobactrum capable of efficiently degrading glyphosate and application of human ochrobactrum
  • Human ochrobactrum capable of efficiently degrading glyphosate and application of human ochrobactrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Isolation and identification of Ochrobactrum anthropi A-1

[0043] 1. Screening and isolation of glyphosate-degrading strains

[0044]Activated sludge was collected from the wastewater treatment tank of a pesticide factory in Huadu District, Guangzhou, and 5 g of activated sludge sample was weighed and added to 50 mL of the above-mentioned MSM liquid medium containing glyphosate (50 mg / L). After culturing for 7 days at 30°C and 200 r / min, 2% of the previous round of culture medium was inoculated into new MSM medium each time, and the mass concentration of pesticides was increased from 50 mg / L to 100 mg / L, 200 mg / L, 400mg / L, 800mg / L for continuous enrichment culture. Then, the transferred culture medium was diluted 4 times and spread on MSM solid plates containing 400 and 800 mg / L glyphosate, and cultured upside down at 30°C for 2 d. After a single colony grew on the plate, pick a single colony on LB solid medium for multiple streak purification, and then use...

Embodiment 2

[0060] Example 2 Experiment on the degradation effect of Bacillus hominis A-1 on glyphosate

[0061] 1. Experimental method

[0062] (1) Preparation of seed liquid: Bacillus hominis A-1 purified in Example 1 was inserted into LB liquid medium containing 5 mL, activated and cultured to logarithmic phase overnight, centrifuged at 4000 rpm, and the cells were treated with sterile physiological The cells were washed twice with saline (0.9% NaCl) and used as inoculum. Resuspend the bacteria with sterile saline to adjust the OD of the bacteria 600 The value is 1.0.

[0063] (2) Determination of degradation performance: 1 mL of the above bacterial solution was inoculated into 50 mL of MSM culture solution containing glyphosate (400 mg / L), and no inoculation was used as a control, and each group was repeated three times. Incubate at 30°C and 200rpm for 72h in a constant temperature shaker, take samples every 12h, and use a UV-Vis spectrophotometer to measure the growth of P. homini...

Embodiment 3

[0084] Example 3 Effects of different culture conditions on the degradation of glyphosate by Bacillus hominis A-1

[0085] 1. Experimental method

[0086] The Bacillus pallidum A-1 obtained in Example 1 was activated in liquid LB medium, cultured at 30° C. in a constant temperature shaker at 200 r / min in the dark for 12 h, and centrifuged at 8000 r / min for 10 min to collect the bacteria, and use sterile physiological After washing 3 times with saline, resuspend and adjust the OD of the bacterial suspension 600 A value of 1.0 is reserved for later use. Initial pH (5.0, 6.0, 7.0, 8.0 and 9.0), inoculum size (1%, 2%, 3%, 4% and 5%), temperature (20°C, 25°C, 30°C, 35°C and 40°C) were investigated separately ℃), initial concentration of glyphosate (25, 50, 100, 200, 400 and 800 mg / L) on the degradation of glyphosate by Bacillus hominis A-1. Subsequently, samples were taken every 12 hours to detect the concentration of residual glyphosate. Taking no inoculation as a control, eac...

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Abstract

The invention discloses Ochrobactrum anthropi (Ochrobactrum anthropi) capable of efficiently degrading glyphosate and an application of the Ochrobactrum anthropi. The human ochrobactrum anthropi A-1 capable of efficiently degrading glyphosate is obtained through screening, separation and research, the strain is preserved in Guangdong Microbial Culture Collection Center on July 21, 2021, and the preservation number of the strain is GDMCC NO: 61823. After the strain is directly applied for 24 hours, the residual amount of glyphosate in a water-sediment system can be reduced by 93% or above, glyphosate can be completely degraded within 36 hours, and the strain can be used for repairing natural environments such as water bodies and soil polluted by glyphosate. The strain A-1 provided by the invention enriches a germplasm resource library of pesticide degrading bacteria, has an important application value in the bioremediation of water bodies and soil polluted by glyphosate residues, and provides a new development way for breaking through the bottleneck of existing pesticide residue pollution treatment; and a theoretical basis and a practical basis are provided for developing a green and safe glyphosate pesticide residue removal technology.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation. More specifically, it relates to Bacillus hominis for efficiently degrading glyphosate and its application. Background technique [0002] Glyphosate (N-phosphonomethylglycine) is a broad-spectrum biocidal and systemic herbicide developed by Monsanto in 1971. Because of its high efficiency, low toxicity and low cost, it has been widely used in agriculture, forestry and animal husbandry. So far, glyphosate has become the most widely used herbicide variety with the largest output and the highest annual sales in the world. Due to its long-term or improper application of damage to non-target organisms and the environment cannot be ignored, more and more studies have shown that glyphosate is not an environmentally friendly pesticide, and it has certain toxicity to non-photosynthetic organisms. It is affected by various factors such as the type of preparation, biological species, and en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01
CPCC12N1/20C02F3/34B09C1/10
Inventor 陈少华张文平李嘉仪黄耀华吴思仪钟剑锋陈永圣
Owner SOUTH CHINA AGRI UNIV
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