Azoxystrobin degrading bacterium and microbial inoculum produced by using degrading bacterium and applications of degrading bacterium

A technology of azoxystrobin and bacterial strains, which is applied in the field of environmental microorganisms and agriculture, and can solve problems such as bioremediation of pesticide residue pollution that cannot be satisfied

Active Publication Date: 2015-05-06
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity of pesticide structures, microbial degradation of pesticides is often highly targeted, resulting in the existing resource pool of degrading bacteria far from meeting the actual needs of bioremediation of pesticide residue pollution.
At present, there are no degrading microorganisms and related degradation preparations specifically targeting azoxystrobin

Method used

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  • Azoxystrobin degrading bacterium and microbial inoculum produced by using degrading bacterium and applications of degrading bacterium
  • Azoxystrobin degrading bacterium and microbial inoculum produced by using degrading bacterium and applications of degrading bacterium
  • Azoxystrobin degrading bacterium and microbial inoculum produced by using degrading bacterium and applications of degrading bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of Pallidum pallidus SH14

[0032] The inventors isolated and screened the azoxystrobin-degrading bacteria by enrichment culture method. Environmental samples for enrichment of azoxystrobin were collected from activated sludge from sewage treatment ponds in Singapore. Take 5 g of the activated sludge sample and add 50 mL of basal medium (the formula is: (NH 4 ) 2 SO 4 , 2 g; MgSO4, 0.2 g; CaCl 2 , 0.01 g; FeSO 4 , 0.005 g; MnCl 2 , 0.002 g; K 2 HPO 4 , 10.5 g; KH 2 PO 4 , 4.5 g in 1000 mL distilled water, pH 7.5). At the same time, the mother solution of azoxystrobin original drug (DMSO as solvent) was added to make the final mass concentration of azoxystrobin 50 mg / L, and the degrading microorganisms were enriched and cultured at 30 °C and 150 r / min on a shaking table. After culturing for 7 days, transfer them to the second batch of basal medium containing 100 mg / L azoxystrobin at a 10% inoculum size. After culturing u...

Embodiment 2

[0038] Example 2 Preparation of Pallidum pallidus SH14 liquid preparation

[0039] The production process of using the above-mentioned Pallidum pallidum SH14 to prepare the liquid preparation is as follows: slant seed—shake flask seed liquid—seed tank cultivation—production tank fermentation—package as liquid bacterial preparation.

[0040] The strains of Paleobacterium pallidum SH14 were activated on LB solid plate, and inoculated on the inclined surface of the test tube for later use. Inoculate the test tube species of Pallidum pallidum SH14 into 1000 mL shake flask containing 250 mL of LB medium, the formula of which is: yeast extract 0.5%, peptone 1%, NaCl 1%, pH value 7.5. 30 ° C is shaken at a constant temperature to the logarithmic phase, and the seed tank is prepared to be inoculated, and the liquid volume of the seed tank is 70%. At 1.1 Kg / cm2 after feeding 3 Under the pressure of 121 ° C, high-pressure damp heat sterilization under the condition of 121 ° C, af...

Embodiment 3

[0042] Example 3 The biodegradation effect of azoxystrobin by Bacillus paleus SH14

[0043] Azoxystrobin was added to the basal medium (same as Example 1) to make the final concentration 50 mg / L; the inoculum concentration was 1.0×10 7 CFU / mL bacterial agent, and set the culture medium without inoculation as the control, culture at 30 °C for 0-5 days, take samples regularly, use HPLC method to measure the residual amount of pesticides and calculate the degradation rate, the results are shown in Table 1 and image 3 shown. The results showed that P.pallidum SH14 could effectively degrade azoxystrobin in a short period of time, and the degradation rate of azoxystrobin reached 76.8% within 3 days, and 86.3% within 5 days. degradation effect.

[0044]

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Abstract

The invention discloses an azoxystrobin degrading bacterium and a microbial inoculum produced by using the degrading bacterium and applications of the degrading bacterium. The strain is ochrobactrum anthropi (Ochrobactrum (anthropi) SH14, the strain is preserved in China Center For Type Culture Collection on December 20, 2013, and the Preservation Number is CCTCC NO: M 2013681. The strain can effectively degrade azoxystrobin pesticide residues in a short time, so that the strain can be used for restoring natural environments such as water and soil and the like polluted by azoxystrobin. A preparation prepared by using the strain has the advantages of low production cost, easiness for use, good removal effect, and the like. By using the microbial inoculum, the residual quantity of azoxystrobin in water and soil can be reduced by over 85% in a short period of time, so that natural environments polluted by azoxystrobin are effectively restored, and the excessive pesticide residue problem and the environmental pollution problem in agricultural production are solved, thereby protecting the ecological environment and the human health.

Description

technical field [0001] The invention relates to the fields of applied environmental microorganisms and agriculture, in particular to an azoxystrobin-degrading bacterium and the bacterial agent produced by it and its application, which is specifically used to repair natural environments such as water bodies or soil polluted by azoxystrobin, and protect the ecological environment and human health . Background technique [0002] Azoxystrobin, also known as Amida, is one of the most commonly used methoxyacrylate fungicides. Azoxystrobin was launched on the market in 1996 and put into agricultural production applications. It has been registered and used in more than 80 crop diseases in 72 countries around the world. The fungicide has an unprecedented ultra-broad bactericidal spectrum, and has good antibacterial activity against most of the pathogenic fungi in Ascomycetes, Basidiomycetes, Deuteromycetes and Oomycetes, and has been widely used in recent years. It is the main fung...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 陈少华刘诗胤廖立胜程莹莹王惠杉
Owner SOUTH CHINA AGRI UNIV
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