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Human pallid bacillus ZJB-061 and uses thereof

A technology of ZJB-061 and Paleobacterium hominis, applied in the fields of application, bacteria, biochemical equipment and methods, etc., can solve the problems of unfriendly environment, low yield, large loss of reagents, etc.

Inactive Publication Date: 2007-10-31
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] The above three methods all need strong alkali or strong acid to participate in the reaction, the reagent loss is large, the environment is not friendly, and the yield is not high

Method used

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  • Human pallid bacillus ZJB-061 and uses thereof
  • Human pallid bacillus ZJB-061 and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Inoculate 100mL of sterilized fermentation medium with human paleobacterium ZJB-061 from the slant: glucose 10g / L, MgCl 2 0.4g / L, Na 2 HPO 4 2g / L, KH 2 PO 4 1g / L, yeast extract 3g / L. Then add β-aminopropionitrile with a final concentration of 44.7mmol / L. After culturing for 4 days, the fermentation broth was refrigerated and centrifuged (4°C, 13000rpm, 10min), the supernatant was removed, washed with phosphate buffer, and centrifuged in the same way. Take 0.5 g of wet bacteria obtained by centrifugation, add 10 mL of phosphate buffer, shake and mix in a 50 mL Erlenmeyer flask with a stopper, add 0.447 mmol (ie 30 μL) of β-aminopropionitrile, and transform in a water bath at 30 °C for 2.5 h. After centrifugation, the supernatant was assayed by pre-column derivatization by high-performance liquid chromatography. The conversion rate was 98%.

[0054] The transformation solution was centrifuged, the supernatant was first adjusted to pH 4, passed through the column ...

Embodiment 2

[0056] Inoculate 100mL of fermented and sterilized culture medium from the slant with Pallidum human ZJB-061: glycerol 4g / L, MgCl 2 0.4g / L, Na 2 HPO 4 2g / L, KH 2 PO 4 1g / L, yeast extract 3g / L. Then add β-aminopropionitrile with a final concentration of 44.7mmol / L. After culturing for 5 days, the fermentation broth was refrigerated and centrifuged (4°C, 13000rpm, 10min), the supernatant was removed, washed with phosphate buffer, and centrifuged in the same way. Take 0.5 g of wet bacteria obtained by centrifugation, add 10 mL of phosphate buffer, shake and mix in a 50 mL Erlenmeyer flask with a stopper, add 0.746 mmol (ie 50 μL) of β-aminopropionitrile, and transform in a water bath at 30 ° C for 6 h. After centrifugation, the supernatant was assayed by pre-column derivatization by high-performance liquid chromatography. The conversion rate was 92%.

Embodiment 3

[0058] Inoculate Pallidum human ZJB-061 into 100mL fermented and sterilized culture medium from the slant: glucose 8g / L, MgCl 2 0.3g / L, Na 2 HPO 4 1.5g / L, KH 2 PO 41.5g / L, then add β-aminopropionitrile with a final concentration of 44.7mmol / L, culture for 4 days and then refrigerate and centrifuge the fermentation broth (4°C, 13000rpm, 10min), remove the supernatant, wash with phosphate buffer, and centrifuge in the same way . Take 0.5 g of wet bacteria obtained by centrifugation, add 10 mL of phosphate buffer, shake and mix in a 50 mL Erlenmeyer flask with a stopper, add 0.447 mmol (ie 30 μL) of β-aminopropionitrile, and transform in a water bath at 30 °C for 2.5 h. Centrifuge and detect by high performance liquid chromatography. The conversion rate reached 91.2%.

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Abstract

The invention discloses a microbe strain human pallid bacillus ZJB-061(Ochrobactrum anthropi ZJB-061) and appliance in beta-alanien preparation, which is characterized by the following: keeping human pale bacillus ZJB-061 in Chinese typical culture preserve center with the preserve number at CCTCC No: M 206039 and preserve date at 20060414; seeding human pale bacillus ZJB-061 bacterial in ferment cultural medium; adding light beta-amido ethyl cyanide into ferment culture medium; proceeding ferment culture; centrifuging the ferment liquid; getting thallus; adding bacterial cell or treated bacterial cell into beta-amido ethyl cyanide solution; proceeding hydrolytic reaction; separating inverting liquid; getting beta-alanien. This invention can replace chemosynthesis method, which possesses high conversion rate, good environment and green course.

Description

(1) Technical field [0001] The invention relates to a new microorganism strain Ochrobactrum anthropi ZJB-061 (Ochrobactrum anthropi ZJB-061) screened from soil, and its application in catalyzing β-aminopropionitrile to prepare β-alanine. (2) Background technology [0002] β-alanine is an important intermediate in the preparation of calcium pantothenate, vitamin B 3 (Pantothenic acid) is composed of pantothenic acid and β-alanine, which exists in all tissues. It is a component of coenzyme A and an indispensable component in energy metabolism in the body. Pantothenic acid participates in carbohydrate, fat and protein metabolism, especially plays a very important role in the synthesis and metabolism of fat. Pantothenic acid is also necessary for the formation of acetylcholine. The lack of pantothenic acid can reduce the growth rate of animals, damage the skin, disturb the nervous system, and hinder the formation of antibodies. [0003] β-Alanine is also a precursor for the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31C12P13/04
Inventor 郑裕国徐亚蓉柳志强沈寅初
Owner ZHEJIANG UNIV OF TECH
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