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Ochrobactrum anthropi DW3 with function of degrading benzo[ghi] perylene

A technology of Bacillus pallidum and lupine, applied in the field of a strain of Bacillus pallidum with the function of degrading benzo[ghi]perylene, can solve the problems of restricting development and application, lack of efficient polycyclic aromatic hydrocarbon degrading bacteria, etc.

Inactive Publication Date: 2015-05-20
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

[0003]However, due to the lack of efficient PAH-degrading bacteria, the further development and application of PAH-contaminated soil bioremediation technology is restricted

Method used

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Examples

Experimental program
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Embodiment Construction

[0019] Add oil-contaminated soil to the open glass bottle containing benzo[ghi]perylene and basal medium to enrich and domesticate benzo[ghi]perylene-degrading microorganisms, and add benzo[ghi]perylene and basal medium after 30 days The conical flasks of culture medium, trace metal solution, vitamin C solution and adsorbent are inserted into enriched and domesticated soil. Cultivate in an oscillator at a temperature of 25-30°C and a speed of 100r / min for 5-7 days, then carry out cycle transfer, and after the number of cycles is more than 8 times, a degrading bacteria group that can efficiently remove benzo[ghi]perylene can be obtained . Make the bottom plate of agar solid medium with benzo[ghi]perylene as the only carbon source and energy source in the ultra-clean workbench, and make the top plate of agar solid medium containing benzo[ghi]perylene-degrading bacteria on the bottom plate, Culture the prepared medium plate in the incubator for 10-15 days. In the ultra-clean wo...

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Abstract

The invention discloses a bacterial strain with a function of degrading benzo[ghi] perylene, and belongs to the technical field of biodegradation processing. The benzo[ghi] perylene degrading bacterium is identified as ochrobactrum anthropi DW3 with a culture preservation number of CGMCC No.8623. The bacterial strain is prepared into a bacterial suspension with bacteria content of 1.25*10<8>CFU / ml, so that a degradation removal rate for the benzo[ghi] perylene with initial concentration of 0.11mg / L after 7 days is 48.7%.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, and in particular relates to a strain of Paleobacterium lupinus capable of degrading benzo[ghi]perylene. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of condensed ring compounds containing two or more benzene rings arranged in linear, angular or clustered shapes, with hydrophobicity, low solubility, low vapor pressure, High melting point and boiling point, high octanol-water partition coefficient and other characteristics. Polycyclic aromatic hydrocarbons have strong carcinogenic, teratogenic and mutagenic effects. Generally speaking, low-molecular-weight PAHs with 2-3 rings have significant acute toxicity, while some high-molecular-weight PAHs have potential carcinogenicity. Due to the presence of conjugated large π-bond electron systems in PAHs molecules, they have high stability,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/32
CPCB09C1/10C02F3/34C02F2101/327C12N1/205C12R2001/01
Inventor 谢恩郑蕾朱宜丁爱中豆俊峰
Owner BEIJING NORMAL UNIVERSITY
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