Bacillus amyloliquefaciens producing lipopeptide to promote fungi apoptosis and lipopeptide prepared by the same and use

Abstract

The invention belongs to the technical field of agricultural microbiology, and relates to the fields of biochemistry and molecular biology simultaneously. Through separation, a bacillus amyloliquefaciens strain CH1 for producing a growth-promoting fungus apoptosis lipopeptide is obtained, which is preserved in the China Center for Type Culture Collection (CCTCC) with the preserving number of CCTCC: M208127. A bioactive substance is obtained from a culture of the bacillus amyloliquefaciens strain CH1 and is characterized as the growth-promoting fungus apoptosis lipopeptide. The molecular weight of the lipopeptide is 1,065 or a homologue thereof. The lipopeptide has an infrared spectrum as shown in Figure 6 in the instruction and consists of 9 carbon alkyls and 8 amino acids, wherein the seventh alkyl carbon and the last amino acid form a cyclic peptide through an oxygen atom. The bacillus amyloliquefaciens strain and the lipopeptide prepared from the same are tested on the biological application prospect in apoptosis.

Description

technical field

[0001] The invention belongs to the technical field of agricultural microbiology, and specifically relates to the isolation and screening of a strain of Bacillus amyloliquefaciens producing lipopeptides that promote fungal apoptosis and the preparation of active substances. The invention also relates to some technical fields of biochemistry and molecular biology. Background technique

[0002] Many bacillus, such as Bacillus subtilis (Bacilus subtilis), Bacillus mycoides (B.mycoides) and Bacillus amyloliquefaciens (B.amyloliquefaciens), can inhibit the growth of many plant pathogenic fungi and human pathogenic fungi. Many of these antifungal substances have been identified as having the characteristics of cyclic lipopeptide surfactants. These lipopeptides can inhibit the growth of many fungi, including Aspergillus (Aspergillus), Fusarium (Fusarium), Rhizoctonia (Rhizoctonia) and some yeasts (Candida). The antitumor activity of some lipopeptides has also rece...

Examples

Embodiment 1

[0035] 1. Screening and isolation of Bacillus amyloliquefaciens:

[0036] Several rice plants at the tillering stage were taken from the paddy fields of Huazhong Agricultural University in Wuhan, Hubei Province, China, and the roots were separated after washing. Rinse the outer surface of the rice root with tap water, then rinse it three times with sterile water, then soak it in 70% ethanol for 30 seconds, take it out, rinse it three times with sterile water, and cut the rice root into about 1mm with sterile scissors Then put the fragments of the same size on PDA medium (containing 200g potato boiled liquid, 20g glucose, 20g agar, add distilled water to 1L) and incubate at 28°C for 48h. The isolated bacteria were cultured with PD liquid medium (containing 200g potato boiled liquid, 20g glucose, and distilled water to 1L) at 28°C, shaken at 180rpm for 48h, centrifuged, and the supernatant was collected and filtered through a 0.22μm membrane After filtering, mix it with PDA med...

technical field

[0001] The invention belongs to the technical field of agricultural microbiology, and specifically relates to the isolation and screening of a strain of Bacillus amyloliquefaciens producing lipopeptides that promote fungal apoptosis and the preparation of active substances. The invention also relates to some technical fields of biochemistry and molecular biology. Background technique

[0002] Many bacillus, such as Bacillus subtilis (Bacilus subtilis), Bacillus mycoides (B.mycoides) and Bacillus amyloliquefaciens (B.amyloliquefaciens), can inhibit the growth of many plant pathogenic fungi and human pathogenic fungi. Many of these antifungal substances have been identified as having the characteristics of cyclic lipopeptide surfactants. These lipopeptides can inhibit the growth of many fungi, including Aspergillus (Aspergillus), Fusarium (Fusarium), Rhizoctonia (Rhizoctonia) and some yeasts (Candida). The antitumor activity of some lipopeptides has also rece...

Examples

Embodiment 1

[0035] 1. Screening and isolation of Bacillus amyloliquefaciens:

[0036] Several rice plants at the tillering stage were taken from the paddy fields of Huazhong Agricultural University in Wuhan, Hubei Province, China, and the roots were separated after washing. Rinse the outer surface of the rice root with tap water, then rinse it three times with sterile water, then soak it in 70% ethanol for 30 seconds, take it out, rinse it three times with sterile water, and cut the rice root into about 1mm with sterile scissors Then put the fragments of the same size on PDA medium (containing 200g potato boiled liquid, 20g glucose, 20g agar, add distilled water to 1L) and incubate at 28°C for 48h. The isolated bacteria were cultured with PD liquid medium (containing 200g potato boiled liquid, 20g glucose, and distilled water to 1L) at 28°C, shaken at 180rpm for 48h, centrifuged, and the supernatant was collected and filtered through a 0.22μm membrane After filtering, mix it with PDA med...

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