Preparation method of bacillus licheniformis raw powder with 100 billion live spores per gram

A technology of bacillus licheniformis and spores, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of easy contamination by miscellaneous bacteria, extensive management, unstable product quality, etc., and achieve process safety and method easy effect

Active Publication Date: 2010-10-27
WUHAN KERNEL BIO-TECH CO LTD
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Problems solved by technology

The solid fermentation method is to inoculate probiotics on solid medium for fermentation and cultivation. Its advantages...

Method used

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Embodiment Construction

[0025] A kind of preparation method of 1,000 billion living spores per gram of bacillus licheniformis former powder, its steps are:

[0026] 1. Optimization of medium for high-yield BLKN-08:

[0027]Response surface experiments designed by Box-Behken were adopted, and the number of spores (cfu) was used as the screening index. Firstly, 10 carbon sources (corn starch, glucose, lactose, sucrose, maltose, mannitol, rice flour, potato starch, etc. , dextrin, corn flour,); 13 nitrogen sources (hydrolyzed plant compound amino acid, cottonseed flour, soybean meal, peanut meal, peptone, yeast powder, corn steep liquor powder, corn steep liquor, fish meal, soybean protein, sodium glutamate, Tianmen Partic acid, ammonium sulfate,); 9 trace elements: cobalt chloride (CoCl 2 ), calcium carbonate (CaCO 3 ), magnesium sulfate (MgSO 4 ), Potassium dihydrogen phosphate (KH 2 PO 4 ), sodium chloride (NaCl), ferrous sulfate (FeSO 4 ), manganese chloride (MnCl 2 ), copper sulfate (CuSO 4...

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Abstract

The invention discloses a preparation method of bacillus licheniformis raw powder with 100 billion live spores per gram. The method comprises the following steps of: (A) screening a fermentation culture medium of a strain BL KN-08, designing an experiment by adopting a response surface method invented by Box-Behken, and using a spore number as a screening index to obtain a culture medium formula beneficial to improving the spore number; (B) increasing the fermentation biomass, carrying out batch fermentation through the culture medium formula screened by using the response surface method to determine an optimal fermentation process beneficial to generating spores, and further increasing the spore number through fed-batch fermentation; and (C) extracting and recovering the spores. The bacillus licheniformis raw powder prepared by using the process has the advantages of clean and safe production, high recovery rate, stable product spore number, and the like, and the live spore number of the powder reaches 100 billions per gram.

Description

technical field [0001] The invention relates to new fields such as purification of polluted water, deodorization of garbage, etc., and more specifically relates to a preparation method of bacillus licheniformis raw powder (1,000 billion live spores / gram), which will be widely used in the feed industry and aquaculture. As the functions of bacillus are still being discovered, the scope of application is also expanding, and it has strong competitiveness in the domestic and international markets. Background technique [0002] Since the 1950s, antibiotics have played a major role as disease treatments and animal growth promoters. However, due to the increasingly serious negative effects of antibiotics, the World Health Organization has banned the use of any antibacterial drugs in feed, and some developed countries have clear regulations on the use of veterinary antibiotics. Feed and aquaculture have been greatly impacted by adding antibiotics to prevent and promote animal growth...

Claims

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Application Information

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IPC IPC(8): A23K1/16C12N1/20C12R1/10
Inventor 陈振民刘华梅李青杨克华谢翔程治国刘荷梅陈又香余娜王巧梅黄志远谢攀龚永华李坤闫世君张敬明
Owner WUHAN KERNEL BIO-TECH CO LTD
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