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Agrobacterium T29 capable of realizing high efficiency activating of mineral elements and heavy metal cadmium

A technology of Agrobacterium and mineral elements, applied in the field of biological treatment of soil pollution, to achieve the effect of promoting plant growth and improving efficiency

Active Publication Date: 2018-09-04
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the prior art, the research on screening out Agrobacterium from farmland soil and specifically using Agrobacterium to activate heavy metal cadmium has not been reported.

Method used

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  • Agrobacterium T29 capable of realizing high efficiency activating of mineral elements and heavy metal cadmium
  • Agrobacterium T29 capable of realizing high efficiency activating of mineral elements and heavy metal cadmium

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Example 1 Isolation and Identification of Bacterial Strains

[0033] A high-efficiency cadmium-dissolving Agrobacterium provided by the present embodiment is formed by screening in the following manner:

[0034] 1. Strain isolation

[0035]Take a 1L beaker and add quantitative deionized water, weigh the ingredients according to the screening medium formula, and add them to the water in turn. Divide each 100mL medium into Erlenmeyer flasks, then add 1g of potassium feldspar to the medium and adjust its pH to 7.0-7.5 with dilute NaOH solution, add agar, wrap, and sterilize at 121°C for 20min. In the petri dish, the solid medium (plate) is formed after the medium cools down. The rhizosphere soil of amaranth polluted by heavy metal cadmium was collected, 10g fresh soil sample was weighed, wrapped in 90mL sterile water (containing glass beads), and transferred to a shaker at 28°C, shaking at 180r / min for 20min. Make a bacterial suspension. Pipette 1mL of bacterial suspen...

Embodiment 2

[0044] Example 2 Activation of mineral potassium in soil by Agrobacterium T29

[0045] The Agrobacterium obtained by screening in Example 1 was confirmed to have a better effect of activating the mineral potassium (potassium feldspar) in the soil through the following experiments.

[0046] 1. Pick the Agrobacterium T29 strain from the preserved slant, inoculate it in LB medium (solid) for activation, and cultivate it for 24 hours. T29 was picked from the solid medium, inoculated into LB medium (liquid) for enrichment, transferred to a shaker at 28°C, and cultured at 150r / min for 18h. Obtain the bacterium liquid after enrichment or make bacterium agent, obtain colony number by dilution plate counting method and be 7 * 10^ 8 CFU / mL. Inoculate the enriched bacterial solution into 100mL potassium solution medium according to 2% (2mL) of the medium volume, and set no bacteria as the control at the same time and set up 3 parallels, in a shaker at 28°C, 150r / min Shake for 7 days. ...

Embodiment 3

[0048] Example 3 Determination of the ability of Agrobacterium T29 to activate calcium phosphate and cadmium carbonate

[0049] 1. Prepare functional medium (without water-soluble phosphate ion and cadmium ion), divide each 100mL medium into Erlenmeyer flasks, add 0.0668g Ca 3 (PO 4 ) 2 with 0.0060g CdCO 3 in the culture medium. Use dilute NaOH solution to adjust its pH to 7.0-7.5, sterilize at 120°C for 30 minutes, and make a cadmium-dissolving medium for later use. For the preparation of the bacterial suspension, refer to the method in Example 2, inoculate the bacterial suspension into the cadmium-dissolving medium at a volume of 2% (2 mL), and set no bacteria as a control at the same time, and set 3 parallels respectively, a total of 6 Samples were transferred to a constant temperature incubator for 7 days at 28°C and 150r / min. Take it out, dilute it to a 100mL volumetric flask, centrifuge the culture solution at 4000r / min for 10min and pass it through a 0.22um filter ...

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Abstract

The invention discloses an agrobacterium sp. T29 capable of realizing high efficiency activating of mineral elements and heavy metal cadmium. The agrobacterium sp. T29 is preserved at Guangdong culture collection center (GDMCC) on 29th, Jan., 2018, and the preservation number is GDMCC No:60321. The agrobacterium sp. T29 is capable of realizing high efficiency activating of mineral elements potassium and phosphor and heavy metal cadmium. The agrobacterium sp. T29 is used for root activating of soil mineral nutrients and promoting plant growth, and is capable of dissolving soil cadmium effectively, and increasing hyperaccumulator plant soil heavy metal cadmium repairing efficiency. In soil heavy metal cadmium activating process using the agrobacterium sp. T29, no pollutant harmful for the environment is discharged, and the application prospect in the field of soil improvement is promising.

Description

technical field [0001] The invention relates to the technical field of biological treatment of soil pollution, more specifically, relates to a strain of Agrobacterium ( Agrobacterium sp.) T29. Background technique [0002] my country's farmland soil is seriously polluted by heavy metals, among which cadmium is the main pollutant. Extraction of cadmium hyperaccumulator plants is one of the most promising technical approaches for soil heavy metal remediation. Sedum sedum, Solanum nigrum, and Sedum sedum are the most reported hyperaccumulator plants used in the extraction and remediation of soil cadmium pollution in China. However, the common problem of these hyperaccumulators is that the enrichment coefficient of cadmium in weakly acidic or neutral soils is significantly lower than that in acidic soils, and the extraction efficiency is low, which seriously limits their practical engineering applications. The low activity of cadmium in weakly acidic or neutral soil is the k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/20C12N1/205C12R2001/01
Inventor 李取生何宝燕王立立吕耀周雪芳
Owner JINAN UNIVERSITY
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