Ochrobacterum sp. MGJ11, and method of immobilizing heavy metal cadmium in soil therewith

A pale bacillus, soil technology, applied in the restoration of polluted soil, microorganism-based methods, botanical equipment and methods, etc., can solve the problem of no restoration of cadmium-contaminated soil, etc., to enhance plant stress tolerance and promote plant growth. Growth, reducing the effect of cadmium enrichment

Active Publication Date: 2016-06-15
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, there is no report on the application of P. pall...

Method used

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  • Ochrobacterum sp. MGJ11, and method of immobilizing heavy metal cadmium in soil therewith
  • Ochrobacterum sp. MGJ11, and method of immobilizing heavy metal cadmium in soil therewith
  • Ochrobacterum sp. MGJ11, and method of immobilizing heavy metal cadmium in soil therewith

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1 contains the bacterial agent of pale bacillus MGJ11

[0027] The strain was isolated from the soybean root system at the edge of the Panzhihua vanadium-titanium magnet tailings pond, and it was found that it had strong tolerance to cadmium and could secrete the plant growth hormone 3-indoleacetic acid (IAA). The plant rhizosphere inoculated with the bacteria can produce the plant growth hormone IAA to promote plant growth, reduce the enrichment of Cd in the soil by plants to alleviate the stress of cadmium on plants, thereby fixing the cadmium in the plant rhizosphere soil, enhancing Stress tolerance of plants.

[0028] The strain was identified as Ochrobactrum sp., numbered MGJ11, and preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (CGMCC for short, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Institute of Microbiology, Zip Code: 100101), the...

Embodiment 2

[0031] Embodiment 2 contains the bacterial fertilizer of Pallidum MGJ11

[0032] Please start from the bacterial agent described in Example 1, and supplement the preparation method of the bacterial fertilizer.

[0033] Please explain the organic fertilizer mixed with the bacterial agent, so that those skilled in the art can reproduce this embodiment according to the description.

Embodiment 3

[0034] Example 3 Microbial immobilization of soil heavy metal cadmium and indoor test results

[0035] The bacterium agent described in embodiment 1 is diluted 500 times.

[0036] The greenhouse pot experiment was carried out with vermiculite added with heavy metal cadmium as the soil matrix. Soybeans were planted in cadmium-containing soil, and the diluted bacterial agent was sprayed into the soil around the roots of soybean seedlings, so that the microbial concentration was 10 9 ~10 10 Bacteria cells / plants, and soybean seedlings not inoculated with Paleobacterium pallidum (MGJ11) were used as a negative control, and nitrogen-containing nutrient solution (KNO 3 ,KH 2 PO 4 0.2gL -1 , KCl0.5gL -1 , CaSO 4 2H 2 O0.2gL -1 , CaCO 3 2gL -1 , MgSO 4 ·7H 2 O0.2g, Fe EDTA, H 3 BO 3 1mgL -1 , ZnSO 4 ·7H 2 O1mgL -1 , CuSO 4 ·5H 2 O0.5mgL -1 , MnCl 2 4H 2 O0.5mgL -1 , Na 2 MoO 4 2H 2 O0.1mgL -1 ), and each treatment was repeated three times (that is, three ...

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Abstract

The invention relates to the technical field of microorganism and environment treatment, and particularly discloses an ochrobacterum sp. MGJ11, which is deposited with the CGMCC at Sep 16th, 2015 and is assigned the accession number CGMCC No.11385. The invention also discloses a method of immobilizing heavy metal cadmium in soil with the ochrobacterum sp. MGJ11, wherein the method particularly includes the steps of applying the ochrobacterum sp. MGJ11 to rhizosphere of plants, or mixing powder of the ochrobacterum sp. MGJ11 with seeds and planting the seeds, wherein the concentration of the inoculated microorganism is not less than 10<9> bacterial cells in every plant.

Description

technical field [0001] The invention relates to the technical field of microbes and environmental treatment, in particular, to a method for fixing soil heavy metal cadmium by using the bacillus pallidus MGJ11 Background technique [0002] Sewage irrigation in farmland, application of solid waste, extensive use of chemical fertilizers and pesticides, and human activities such as mining and smelting have caused soil to be more and more polluted by heavy metals. Among them, cadmium (Cd) pollution is particularly obvious (Jiang Chunyu et al., Biological characteristics of a lead-cadmium resistant strain WS34 and its strengthening effect on phytoremediation of lead-contaminated soil[J]. Environmental Science Journal 2008, 28(10) .1961-1968). Cadmium is one of the heavy metal elements that are highly toxic to plants and humans. Long-term exposure to a certain dose of cadmium can cause kidney damage, which can lead to osteoporosis and softening, and can lead to cancer in severe c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08B09C1/10A01N63/02A01P21/00C12R1/01
Inventor 余秀梅李杨昕李艳梅康夏陈强辜运富陈翠平谢雨歆崔永亮
Owner SICHUAN AGRI UNIV
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