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Low-temperature denitrification pseudomonas fluorescens

A technology of Pseudomonas fluorescens and Pseudomonas fluorescens, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of reduced denitrification ability and narrow application range

Inactive Publication Date: 2013-09-04
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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Problems solved by technology

[0004] Biological denitrification efficiency is limited by temperature and nitrogen concentration. Generally, strains have a narrow adaptability to nitrogen concentration, and their denitrification ability decreases at low temperature (<10°C), so they are not suitable for denitrification of low-temperature water source water.

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  • Low-temperature denitrification pseudomonas fluorescens
  • Low-temperature denitrification pseudomonas fluorescens
  • Low-temperature denitrification pseudomonas fluorescens

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specific Embodiment approach 1

[0016] Specific embodiment 1: In this embodiment, a strain of low-temperature denitrification Pseudomonas fluorescens, which is Pseudomonas fluorescens WSW-1001, has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is It is CGMCC No.7476, the preservation date is April 15, 2013, and the preservation address is No. 3, Yard No. 1, Beichen Road, Chaoyang District, Beijing.

[0017] In this embodiment, a strain of low-temperature denitrification Pseudomonas fluorescens was isolated and screened from the Songhua River in Heilongjiang Province in March. Sodium acetate and 5mL trace element solution, 8°C, 160r / min enrichment culture for 5 days, take 1mL enrichment culture solution into the Erlenmeyer flask containing 50mL basic culture solution, 3 parallel samples, 8°C, 160r / min shaking culture After 3 days, the culture solution was diluted to 1000 times, and 100 μL of the culture solution...

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Abstract

The invention discloses low-temperature denitrification pseudomonas fluorescens and relates to pseudomonas fluorescens. The low-temperature denitrification pseudomonas fluorescens are pseudomonas fluorescens WSW-1001 and are collected in China General Microbiological Culture Collection Center (CGMCC), the collection number is CGMCC No.7476, the collection date is April 15th, 2013, and the collection address is No.3, No.1 Courtyard, Beichen Road, Chaoyang District, Beijing. The low-temperature denitrification pseudomonas fluorescens are heterotrophic nitrification aerobic denitrifiers, can be used for degrading NH4+-N, NO--N and NO--N (0.25 mg / L-1314.1 mg / L) with a wide concentration range, can be applied to low-nitrogen pollution source water denitrification, medium- and high-concentration wastewater denitrification and eutrophic water body treatment, and have an important meaning on low-nitrogen pollution source water denitrification, water eutrophication control and biological environment modification.

Description

technical field [0001] The invention relates to a strain of Pseudomonas fluorescens. Background technique [0002] With the rapid development of society and economy, nitrogen pollution in my country's water environment is becoming more and more serious. Domestic sewage, industrial wastewater, aquaculture wastewater and farmland nitrogen fertilizer are the main sources of nitrogen pollution in the water environment. Water nitrogen pollution not only causes serious environmental pollution and economic losses, but even threatens human health and ecological security. Microbial nitrogen removal is considered to be the most economical, effective and promising water nitrogen removal technology due to its advantages of high efficiency, low cost, and no secondary pollution. [0003] Nitrogen in polluted water mainly includes NH 4 + -N, NO 3 - -N and NO 2 - -N inorganic nitrogen and organic nitrogen. Traditional biological denitrification includes ammonification, nitrificatio...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/39
Inventor 张淑梅姜威高娃李晶孟利强赵晓宇陈靖宇曹旭
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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