Low-temperature denitrification pseudomonas fluorescens
A technology of Pseudomonas fluorescens and Pseudomonas fluorescens, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of reduced denitrification ability and narrow application range
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[0016] Specific embodiment 1: In this embodiment, a strain of low-temperature denitrification Pseudomonas fluorescens, which is Pseudomonas fluorescens WSW-1001, has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is It is CGMCC No.7476, the preservation date is April 15, 2013, and the preservation address is No. 3, Yard No. 1, Beichen Road, Chaoyang District, Beijing.
[0017] In this embodiment, a strain of low-temperature denitrification Pseudomonas fluorescens was isolated and screened from the Songhua River in Heilongjiang Province in March. Sodium acetate and 5mL trace element solution, 8°C, 160r / min enrichment culture for 5 days, take 1mL enrichment culture solution into the Erlenmeyer flask containing 50mL basic culture solution, 3 parallel samples, 8°C, 160r / min shaking culture After 3 days, the culture solution was diluted to 1000 times, and 100 μL of the culture solution...
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