Bacillus altitudinis strain and application thereof

A highland bacillus and strain technology, applied in the application, bacteria, fungicides and other directions, can solve the problems of destroying ecological balance, harming agricultural production, harming human health, etc., and achieving the effect of good effect, short cultivation time and easy acquisition.

Pending Publication Date: 2019-07-30
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, people have considered chemical pesticides for the prevention and control of diseases. However, due to the long-term use of chemical pesticides, they have caused potent...

Method used

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  • Bacillus altitudinis strain and application thereof
  • Bacillus altitudinis strain and application thereof
  • Bacillus altitudinis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] - Bacillus highlandii ( Bacillus altitudinis ) Acquisition, identification and preservation of Bag 1.

[0047] (1) Bacillus highlandi ( Bacillus altitudinis ) Acquisition and identification of Bag 1.

[0048] The Bacillus highlandii described in the present invention is isolated from healthy plants and diseased plants in the diseased plots of A. chinensis and their rhizosphere soils. The samples were collected from Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, and were refrigerated at 4°C for future use.

[0049] The rhizosphere bacteria of healthy plants in the diseased plot were isolated and cultured by conventional tissue method: after washing the surface soil of fresh and healthy root tissue of A. chinensis from the diseased plot with distilled water, the root tissue was cut into 0.5cm × 0.5cm tissue blocks. The surface of the plant tissue was disinfected with 0.1% mercuric chloride solution for 1-5 min, then deep-sterilized with 75% alcohol for 1 min...

Embodiment 2

[0060] - by Bacillus highlandi ( Bacillus altitudinis ) Screening of Bag 1 strains.

[0061] (1) Screening of strains

[0062] Antagonists were screened using the criss-cross method. The specific operation is as follows: take Fusarium oxysporum as the indicator bacteria, inoculate the agar block of the pathogenic bacteria in the center of the PDA medium plate, and then inoculate the single bacterial colony obtained by separation and culture at an equidistant distance of 3 cm from the pathogenic bacteria, and each plate is inoculated with 4 strains , 5 replicates were set, and after incubation at 28°C for 3 days, the size of the inhibition zone was observed, and the strain with the best antagonistic effect was selected.

[0063] (2) Observation of results.

[0064] The isolated strains were screened by crisscross method, and the results showed that there were 3 strains that had antagonistic effect on rhizome rot of Astragalus chinensis.

Embodiment 3

[0066] - by Bacillus highlandi ( Bacillus altitudinis ) Determination of the biocontrol effect of the Bag 1 strain.

[0067] (1) Determination of biocontrol effect.

[0068] Confrontation culture method: Using Fusarium oxysporum as the indicator bacteria, first use a hole punch to punch out the activated pathogenic bacteria cake, put it on the plate, and then inoculate the antagonistic bacteria by the point connection method. The distance between the two bacteria is 5 cm. Inoculate the pathogenic bacteria cake, set up 3 repetitions, cultivate in a 28°C incubator, observe the growth of the pathogenic bacteria, measure the inhibition zone, even if the inhibition rate is calculated, and finally screen out the antagonistic bacteria with the best antagonistic effect. The formula for calculating the bacteriostatic rate: bacteriostatic rate (%) = (colony diameter of control pathogenic bacteria - colony diameter of treated pathogenic bacteria) / colony diameter of control pathogenic ...

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Abstract

The invention discloses a bacillus altitudinis strain and application thereof. The bacillus altitudinis strain is classified and named as Bacillus altitudinis, the preservation unit is the China general microbiological culture collection center (CGMCC), the preservation date is December 28th in 2018, the preservation number is CGMCCNo.17046. The bacillus altitudinis strain belongs to the genus ofbacillus, the family of bacillaceae, the order of bacillales, the class of bacilli, the phylum of firmicutes in the domain of bacteria. The bacillus altitudinis strain can be applied in biological control over root rot of plukenetia volubilis.

Description

technical field [0001] The invention belongs to the technical field of microorganism separation and culture, and in particular relates to a strain of Bacillus highlandi and its application. Background technique [0002] Star oil vine seeds contain major components such as oil, protein and amino acids. It also contains antioxidants, vitamin A, vitamin E and protein, which has a significant effect on regulating blood lipids, preventing skin aging and controlling cardiovascular diseases. It does not contain erucic acid and toxins, and can be widely used in food, pharmaceutical and cosmetic processing industries. , is considered to be the best vegetable oil and health care cosmetic product material, won medals at the 2004, 2006 and 2010 World Edible Oil Exhibition, and won the "Golden Title of Plant Brain" in 2007, with broad prospects for development and use. [0003] However, at the beginning of 2015, the planters found that the perennial plants of Astragalus were dying in la...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCC12N1/20A01N63/00C12R2001/07C12N1/205
Inventor 魏玉倩伍建榕马焕成洪英娣马翔王芳潘启强郑艳玲
Owner SOUTHWEST FORESTRY UNIVERSITY
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