Guangzhou pallor bacillus with high-efficiency degradation ability for pyridine

A pale bacillus and pyridine technology, applied in the field of Guangzhou pale bacillus new strains, can solve problems such as the pale bacillus that have not yet been found, and achieve the effects of speeding up the ecological restoration process, low cost, and improving efficiency

Active Publication Date: 2008-10-29
GUANGDONG INST OF MICROORGANISM
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  • Abstract
  • Description
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Problems solved by technology

However, no pyridine-degrading strains have been found in Paleobacter spp.

Method used

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  • Guangzhou pallor bacillus with high-efficiency degradation ability for pyridine

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Experimental program
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Effect test

Embodiment 1

[0026] Add Na to 100ml deionized water 2 HPO 4 .7H 2 O 1.28g, (NH 4 ) 2 SO 4 0.1g, FeSO 4 0.01g, NaCl 0.2g, K 2 HPO 4 0.6g, 0.08g pyridine, sterilized at 121°C for 20min as a culture solution and distributed into 20ml test tubes, 5ml per tube. From preserved Ochrobactrum guangzhouense P 2 CCTCC M 207191 slant bacteria were inoculated into LB liquid medium, and the bacteria were activated on a shaking table at 30°C and 180r / min, so that the number of bacteria reached 10 8 Order of magnitude, inoculate 100 μl of activated bacteria solution into the culture medium, place it at 30°C, and culture it on a shaker at 180r / min. Take 2ml of the culture solution and centrifuge it at 5000r / min for 3min on the 3rd day to remove bacteria. Use DU 640 ultraviolet spectrophotometer (BECKMAN ) Measure the absorbance of pyridine in the culture medium at 256nm. Calculate the degradation rate of pyridine. Paleobacterium cantonensis P 2 The degradation rate of pyridine reaches 90.91%...

Embodiment 2

[0028] Add Na to 100ml deionized water 2 HPO 4 .7H 2 O 1.28g, (NH 4 ) 2 SO 4 0.1g, FeSO 4 0.01g, NaCl 0.2g, K 2 HPO 4 0.6g, 0.1g pyridine, sterilized at 121°C for 20min as a culture solution and distributed into 20ml test tubes, 5ml per tube. From preserved Ochrobactrum guangzhouense P 2 CCTCC M 207191 slant bacteria were inoculated into LB liquid medium, and the bacteria were activated on a shaking table at 30°C and 180r / min, so that the number of bacteria reached 10 8 Order of magnitude, inoculate 100 μl of activated bacteria liquid into the culture medium, place it at 30°C, and cultivate it on a shaker at 180r / min. Take 2ml of the culture solution and centrifuge at 5000r / min for 3min on the 3rd day to remove bacteria. Use a DU 640 ultraviolet spectrophotometer ( Beckman) measured the absorbance of pyridine in the culture medium at 256 nm. Calculate the degradation rate of pyridine. Paleobacterium cantonensis P 2 The degradation rate of pyridine reaches 92.25%...

Embodiment 3

[0030] Add Na to 100ml deionized water 2 HPO 4 .7H 2O 1.28g, (NH 4 ) 2 SO 4 0.1g, FeSO 4 0.01g, NaCl 0.2g, K 2 HPO 4 0.6g, 0.2g pyridine, sterilized at 121°C for 20min as a culture solution and dispensed into 20ml test tubes, 5ml per tube. From preserved Ochrobactrum guangzhouense P 2 CCTCC M 207191 slant bacteria were inoculated into LB liquid medium, and the bacteria were activated on a shaking table at 30°C and 180r / min, so that the number of bacteria reached 10 8 Order of magnitude, inoculate 100 μl of activated bacteria solution into the culture medium, place it at 30°C, and cultivate it on a shaker at 180r / min. Take 2ml of the culture solution and centrifuge at 5000r / min for 3min on the 3rd day to remove bacteria, and use a DU 640 ultraviolet spectrophotometer ( Beckman) measured the absorbance of pyridine in the culture medium at 256 nm. Calculate the degradation rate of pyridine. Paleobacterium cantonensis P 2 The degradation rate of pyridine reaches 95....

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Abstract

The invention provides Ochrobactrum guangzhouense P2 with high capacity in degrading pyridine. The strain as a novel species of Ochrobactrum genus is obtained by separating and purifying sludge of oil and gas factories, and is preserved in China Center for Type Culture Collection with the preservation number of CCTCC M 207191. The strain has higher capacity in degrading pyridine, and can effectively degrade pyridine in sludge, thus lowering the sludge processing cost.

Description

technical field [0001] The invention relates to the field of bacteria, in particular to a new strain of Cantonensis pallidum with high-efficiency degradation of pyridine. Background technique [0002] As a typical refractory organic pollutant, the environmental problems caused by heterocyclic aromatic compounds have attracted more and more attention. Because most of them have "three effects", they are harmful to human health and ecological balance. At present, the diffusion of heterocyclic aromatic compounds in the environment has caused serious pollution of soil and groundwater. An effective method to solve such problems in bioremediation, the use of degrading microorganisms screened from the environment can effectively remove pollutants such as heterocycles and benzene rings in sludge and other environments. [0003] Pyridine, as the most basic and typical heterocyclic aromatic hydrocarbon substance, has become the focus of research, especially focusing on the biodegradat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/01C02F101/38
CPCY02W10/20
Inventor 张国霞孙国萍曾国驱许玫英
Owner GUANGDONG INST OF MICROORGANISM
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