The invention discloses a method for improving primordium inducement uniformity of bottle-cultivated pleurotus eryngii, and relates to the technical field of pleurotus eryngii cultivation. The method comprises the steps that mycelium stimulation is carried out on the bottle-cultivated pleurotus eryngii, and then pleurotus eryngii enters a pleurotus eryngii cultivation room; ventilation and light illumination of the pleurotus eryngii cultivation room are closed, the pleurotus eryngii cultivation room is made to be in the closed dark condition, the temperature is adjusted to range from 8 DEG C to 10 DEG C, the relative humidity is improved to 95%, and the pleurotus eryngii is kept in the pleurotus eryngii cultivation room under the cultivation environment for 24 hours; then the temperature is increased to range from 16 DEG C to 18 DEG C; the light illumination is adjusted to be opened for 30 min after being closed for 60 min every time; the ventilation quantity is adjusted so as to maintain the indoor concentration of carbon diode to be 2000 ppm, and primordium inducement is carried out for 5 days to 8 days. According to the method, the growth characteristics of the pleurotus eryngii under the natural environment are met, the primordium inducement effect of the pleurotus eryngii is improved by reasonably controlling the light illumination, ventilation, temperature and humidity of the pleurotus eryngii cultivation room, the step of transition cultivation is set in the primordium inducement process, the vitality of hyphae can be vigorous, the phenomenon that the growing pleurotus eryngii is not uniform is avoided, water is supplemented in the step of transition cultivation, the hyphae can be positioned, and it is further ensured that the growing pleurotus eryngii is uniform.