Preparation method of low-fluorescent-background assembled gold magnetic composite nanometer particles and application thereof

A composite nanoparticle, assembly-type technology, applied in the fields of nanotechnology, nanomagnetism, nanotechnology, etc., can solve the problems of photothermal conversion properties, insufficient application of fluorescence synergy, and achieve good biocompatibility, reaction Mild conditions and simple operation

Active Publication Date: 2012-07-11
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of gold-magnetic composite particles prepared by many methods in the separation, purification and detection of biomolecules is often limited to the use of the surface of gold nanoparticles as a carrier for binding with biomolecules, and the other properties of gold nanoparticles (such as: near The photothermal conversion properties and fluorescence synergies in the infrared region) are not fully applied

Method used

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  • Preparation method of low-fluorescent-background assembled gold magnetic composite nanometer particles and application thereof
  • Preparation method of low-fluorescent-background assembled gold magnetic composite nanometer particles and application thereof
  • Preparation method of low-fluorescent-background assembled gold magnetic composite nanometer particles and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of assembled gold-magnetic composite nanoparticles

[0038] figure 1 Assembled Fe 3 o 4 SiO 2 The synthesis process of Au-gold magnetic composite nanoparticles includes four steps: ① Preparation of magnetic nano-Fe 3 o 4 Particles; ②Preparation of Fe 3 o 4 SiO 2 particles and modify amino groups on its surface; ③ reduce chloroauric acid with sodium borohydride to obtain small-sized gold nanoparticles; ④ mix the above Fe 3 o 4 SiO 2 suspension and nano-gold suspension and ultrasonically stirred to obtain assembled Fe 3 o 4 SiO 2 Au gold magnetic composite nanoparticles.

[0039] The specific experimental process is as follows.

[0040] (1) Preparation of Fe by soft template method 3 o 4 magnetic nanoparticles. Weigh 1.3 g ferric chloride (FeCl 3 ·6H 2 O), 2.6 g ammonium acetate (NH 4 Ac) and 1.95 g of dextran (mass ratio: 1:2:1.5), were dissolved in 50 mL of ethylene glycol, stirred by magnetic force, then mixed and poured into ...

Embodiment 2

[0046] Example 2 Streptavidin Modified Assembled Gold Magnetic Composite Nanoparticles

[0047] 11-Mercaptoundecanoic acid (11-Mercaptoundecanoic acid, MUDA) with a concentration of 20 mM and the gold magnetic composite nanoparticles prepared in the above-mentioned Example 1 (the mass volume ratio of gold magnetic composite nanoparticles and MUDA is 5:1 ) and shake for 24 hours. After the reaction is complete, wash twice with 95% ethanol and sterile water, and finally disperse in sterile water at a concentration of 4 mg / mL. After that, take a NaOH aqueous solution with a concentration of 0.01 M (the volume ratio of the gold magnetic composite nanoparticle suspension and the NaOH solution is 5:1) and add it to the above-mentioned modified gold magnetic composite nanoparticles to react for 15 minutes. After the reaction is complete, use Wash with sterile water three times. Then add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (1-Ethyl-3-(3-dimethyllaminopropyl) ...

Embodiment 3

[0049] Example 3 Fluorescence background detection of assembled gold-magnetic composite nanoparticles

[0050] The biotin-Cy3 double-labeled oligonucleotide fragment (5'-biotin-TGAAGGAGAAGGTGTCTGCGGGA-Cy3-3') with a concentration of 1 μM and the streptavidin-gold magnetic particles prepared in the above-mentioned Example 2 were mixed in mass ratio Mix 1:2, shake and react at room temperature for 15 minutes. After the reaction, separate magnetically and wash thoroughly with phosphate buffer (PB). Record the fluorescence value of the washing solution after scanning the slice with a scanner until the fluorescence value does not change. image 3 A shows the fluorescence scanning records of each washing solution, row a is the streptavidin-gold magnetic particle supernatant solution without PB buffer washing, row b-f is the streptavidin after washing 1-6 times with PB buffer prime-gold magnetic particle supernatant solution. It can be seen from the figure that after six times of w...

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Abstract

Disclosed are a preparation method of low-fluorescent-background assembled gold magnetic composite nanometer particles and application thereof. The preparation method includes steps of preparing magnetic nanometer Fe3O4; preparing Fe3O4@SiO2 particles, decorating the surfaces of the Fe3O4@SiO2 particles with amino perssad; preparing small-diameter nanometer particles by utilizing sodium borohydride to reduce chloroauric acid; mixing the F33O4@SiO2 particle injectable suspension and the nanometer injectable suspension according to the volume proportion of 1:3, stirring the same ultrasonically, and obtaining assembled Fe3O4@SiO2@Au gold magnetic composite nanometer particles by coordinate bond of nanometer gold particles and the amino perssad on the surface of the Fe3O4@SiO2 particles. The Fe3O4@SiO2@Au gold magnetic composite nanometer particles are prepared by self-assembled technology, and are controllable in size, uniform in distribution, excellent in dispersion, stability and biocompatibility and lower in preparation cost. The preparation process is simple, requires mild reaction conditions, and has the advantages of low cost and convenience in operation.

Description

[0001] technical field [0002] The invention belongs to the field of synthesis of composite nanomaterials, and relates to a composite nanomaterial of an assembled gold-coated magnetic ferrite compound with a low fluorescence background, that is, Fe 3 o 4 SiO 2 The invention relates to Au gold-magnetic composite nanoparticles and a preparation method thereof, in particular to the preparation of gold-magnetic composite nanoparticles suitable for directly detecting fluorescence signals on the surface thereof by adopting a self-assembly technical scheme. [0003] Background technique [0004] Magnetic material is a traditional material with a long history. Studies have shown that when its size enters the nanometer scale, the resulting magnetic nanomaterials will exhibit some special physical phenomena, such as: changes in coercive force, superparamagnetism, and a drop in Curie temperature. Because of these characteristics, magnetic nanoparticles, as an important part of nan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01F1/00B82Y25/00B82Y40/00G01N21/64
Inventor 何农跃曾新江红荣李传燕
Owner SOUTHEAST UNIV
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