The invention discloses a Pu'er tea yeast fermentation technology. The technology comprises the following steps: (1) cleaning, (2) fermentation, (3) tea overturning, (4) stacking, (5) natural drying and (6) refining. According to the invention, Pu'er tea leaves are fermented in a fermentation room with the assistance of yeast, so that the cell membrane permeability of the Pu'er tea is increased, the enzymatic oxidation of polyphenols substances is promoted, the quality of the Pu'er tea is enhanced and the contents of water extracts, tea pigment, amino-acid and the like of the Pu'er tea are increased; as the Pu'er tea is produced through the adoption of the yeast fermentation technology, the fermentation time is distinctly shortened and the production efficiency is improved. The Pu'er tea, produced by adopting the yeast fermentation technology, is bright in color, full in shape, attractive in appearance, rich and strong in scent and rich in nutrition; the Pu'er tea water has a sweet and faint fragrance and is palatable; color, fragrance, taste, shape and nutrition of the original tea leaves are kept.