Preparation method and application of pyracantha fortuneana fruit extract

A technology of extract and Pyracantha, which is applied in the field of preparation of Pyracantha extract, can solve problems such as spots, affecting facial health and appearance, dark yellow skin, etc., and achieves improved extraction rate, low equipment requirements, and low cost Effect

Pending Publication Date: 2021-10-08
INGREDI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although melanin can reduce the damage of ultraviolet rays to the skin, excessive accumulation can easily lead to the appearance of dark yellow skin and spots, seriously affecting the health and beauty of the face

Method used

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  • Preparation method and application of pyracantha fortuneana fruit extract
  • Preparation method and application of pyracantha fortuneana fruit extract
  • Preparation method and application of pyracantha fortuneana fruit extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A preparation method of fire-acantha extract, comprising the following steps:

[0072] (1) enzymolysis; get fireacantha, add compound enzyme and carry out enzymolysis, obtain enzymolysis mixture; Wherein, compound enzyme is made up of polygalacturonase, cellulase and pectinase, and the temperature of enzymolysis is 40°C, the enzymatic hydrolysis time is 2 hours, and the pH value of the enzymatic hydrolysis is 5. The mass ratio of polygalacturonase, cellulase and pectinase is 1:1:1.

[0073] (2) Alcohol extraction; add ethanol to the enzymolysis mixture to heat and reflux extraction, filter, concentrate, and obtain an ethanol extract; wherein, the mass percentage concentration of ethanol during ethanol extraction is 60%, and the number of times of ethanol extraction is 2 times, each time The ethanol extraction time was 2 hours.

[0074] (3) Macroporous adsorption resin purification; the ethanol extract is loaded on the macroporous adsorption resin whose resin model is ...

Embodiment 2

[0077] A preparation method of fire-acantha extract, comprising the following steps:

[0078] (1) enzymolysis; get fireacantha, add compound enzyme and carry out enzymolysis, obtain enzymolysis mixture; Wherein, compound enzyme is made up of polygalacturonase, cellulase and pectinase, and the temperature of enzymolysis is 42°C, the enzymatic hydrolysis time is 3 hours, and the pH value of the enzymatic hydrolysis is 6.5. The mass ratio of polygalacturonase, cellulase and pectinase is 2:1:1.

[0079] (2) Alcohol extraction; add ethanol to the enzymolysis mixture, heat and reflux extraction, filter, concentrate, and obtain an ethanol extract; wherein, the mass percentage concentration of ethanol during ethanol extraction is 80%, and the number of times of ethanol extraction is 4 times, each time The ethanol extraction time was 1 hour.

[0080] (3) Macroporous adsorption resin purification; the ethanol extract is loaded on the macroporous adsorption resin whose resin model is H...

Embodiment 3

[0083] A preparation method of fire-acantha extract, comprising the following steps:

[0084] (1) enzymolysis; get fireacantha, add compound enzyme and carry out enzymolysis, obtain enzymolysis mixture; Wherein, compound enzyme is made up of polygalacturonase, cellulase and pectinase, and the temperature of enzymolysis is 37°C, the enzymatic hydrolysis time is 1 hour, and the pH value of the enzymatic hydrolysis is 4.5. The mass ratio of polygalacturonase, cellulase and pectinase is 1:2:1.

[0085] (2) Alcohol extraction; add ethanol to the enzymolysis mixture, heat and reflux extraction, filter, concentrate, and obtain an ethanol extract; wherein, the mass percentage concentration of ethanol during ethanol extraction is 50%, and the number of times of ethanol extraction is 1 time. The time is 3 hours.

[0086] (3) Macroporous adsorption resin purification; the ethanol extract is loaded on the macroporous adsorption resin whose resin model is HPD600 for purification, and the...

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Abstract

The invention discloses a preparation method of a pyracantha fortuneana fruit extract. The preparation method comprises the following steps of: (1) enzymolysis: taking pyracantha fortuneana fruits, and adding a compound enzyme for enzymolysis to obtain an enzymolysis mixture; (2) alcohol extraction: adding ethanol into the enzymolysis mixture for carrying out extraction, and carrying out filtering and concentrating to obtain an ethanol extract; (3) macroporous adsorption resin purification: loading the ethanol extract on macroporous adsorption resin for purification, and collecting a desorption solution; and (4) drying: concentrating and drying a decomposition and precipitation solution to obtain the product. The preparation method has the advantages that flavone in the pyracantha fortuneana fruit can be effectively extracted, industrial production can be easily realized, and the pyracantha fortuneana fruit extract with the effects of resisting oxidation, brightening and whitening is obtained.

Description

technical field [0001] The invention belongs to the field of daily chemical products, and in particular relates to a preparation method and application of a fire-acantha extract. Background technique [0002] Human beings' concern about self-image and pursuit of beauty has led to an increasing demand for cosmetics. With the progress of the times and the improvement of human living standards, people are paying more and more attention to the effectiveness and safety of cosmetics. "Green and natural" is The focus of current cosmetics. Safe and effective natural plant cosmetic raw materials have a good competitive advantage in the cosmetics market. [0003] Fair skin is a topic of common concern to most oriental women. However, due to various factors such as environmental pollution, ultraviolet rays, and work pressure, problems such as skin pigmentation, chloasma, and skin aging appear. Traditional whitening agents (such as hydroquinone, mercury and its compounds, α-arbutin) h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/02A61Q19/08
CPCA61K8/9789A61Q19/02A61Q19/08A61K2800/805
Inventor 马占林黄文国王媛冯秋红郑晓琼黄灿周戟罗慧毕艳玲
Owner INGREDI BIOTECH CO LTD
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