Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes

a nucleic acid and probe technology, applied in the field ofmolecular biology, can solve the problems of increasing the fluorescence of the detector dye, contaminating and false positives, and affecting the efficiency of the hydrolysis probe in homogeneous assays, and achieves enhanced binding affinities and low fluorescent effects
US20050233360A1Inactive Publication Date: 2005-10-20SIGMA ALDRICH CO LLC
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
SIGMA ALDRICH CO LLC
Publication Date
2005-10-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention comprises novel methods and strategies to detect and / or quantify nucleic acid analytes. The methods involve nucleic acid probes with covalently conjugated dyes, which are attached either at adjacent nucleotides or at the same nucleotide of the probe and novel linker molecules to attach the dyes to the probes. The nucleic acid probes generate a fluorescent signal upon hybridization to complementary nucleic acids based on the interaction of one of the attached dyes, which is either an intercalator or a DNA groove binder, with the formed double stranded DNA. The methods can be applied to a variety of applications including homogeneous assays, real-time PCR monitoring, transcription assays, expression analysis on nucleic acid microarrays and other microarray applications such as genotyping (SNP analysis). The methods further include pH-sensitive nucleic acid probes that provide switchable fluorescence signals that are triggered by a change in the pH of the medium.
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Description

RELATED APPLICATIONS

[0001] This application is a divisional application of U.S. application Ser. No. 10 / 278,047, filed Oct. 21, 2002, which is a non-provisional of Provisional Application Ser. No. 60 / 336,432, filed Oct. 19, 2001, both of which are entitled “Nucleic Acid Probes And Methods To Detect And / Or Quantify Nucleic Acid Analytes” and each of which is incorporated herein by reference in its entirety.FIELD OF INVENTION

[0002] The present invention relates to the field of molecular biology. More specifically, the present invention relates to the field of assays that utilize nucleic acid probes to detect and / or quantify nucleic acid analytes. BACKGROUND OF THE INVENTION

[0003] Advances in DNA technology and sequencing, specifically the sequencing of whole genomes including the human genome, have resulted in a significantly increased need to detect and / or quantify specific nucleic acid sequences. Applications include the fields of in vitro diagnostics, including clinical diagnost...

Claims

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