Dual-fluorescence staining solution for vaginal microorganism detection and application thereof

Abstract

The invention discloses a dual-fluorescence staining solution for vaginal microorganism detection. The dual-fluorescence staining solution for the vaginal microorganism detection comprises an independent dual-fluorescence staining solution A and an independent dual-fluorescence staining solution B, wherein the dual-fluorescence staining solution A is composed of a fluorescence staining agent, an auxiliary staining agent, a staining solution buffer reagent, a bacteriostatic agent and water, and the dual-fluorescence staining solution B is composed of a staining solution buffer reagent, an alkaline regulating reagent, an anti-quenching agent, a bacteriostatic agent and water. The invention has the advantages of being simple in operation process, rapid in detection and easy to observe and recognize after pathogenic microorganism dyeing. Moreover, the interference of contaminated specimens such as bloody specimens, seminal fluid specimens and basal cell specimens on detection results is overcome, and the accuracy and the detection rate are effectively improved.

Description

technical field [0001] The invention relates to the technical field of microbial fluorescent dyeing, in particular to a double fluorescent staining solution for vaginal microorganism detection and its application. Background technique [0002] There are many microbial flora symbiotic in the female lower reproductive tract, which have a natural defense function against the invasion of pathogens. However, when the vaginal microecology is out of balance and the natural defense function is destroyed, pathogens are easy to invade, leading to vaginitis, cervicitis, pelvic inflammatory disease, etc. The occurrence of diseases increases the risk of AIDS and cervical cancer; women with vaginitis will affect the quality of sperm during pregnancy, and even lead to infertility; even during pregnancy, it is easy to cause intrauterine infection, birth canal infection and other links to infect the fetus , newborns, resulting in miscarriage, premature birth, congenital deformities, mental r...

AI Technical Summary

Problems solved by technology

This method has no staining effect on the cells and microorganisms in the sample, and can be distinguished according to the shape of each component. However, due to the complex background of the entire smear, it is sometimes difficult to observe and identify some tiny microorganisms, such as fungal infections.

Moreover, for Trichomonas infection, if the sample is not properly preserved during the inspection process, if the temperature is too low, the Trichomonas will die or its activity will be low, and it will not be easy to be observed under the microscope, which will easily lead to missed diagnosis.

[0005] Gram staining is also one of the microscopic staining methods currently used in some hospitals. This method can clearly observe the microorganisms in the sample, such as the Gram-positive or negative microorganisms. However, this method is complicated to operate and generally requires 4 steps. Dyeing method, and the technical requirements for dyeing in the dyeing process are relatively high. For example, if the decolorization process is excessively decolorized, the results will be inaccurate, so the requirements for medical technicians are higher.

Moreover, because the shape and size of Trichomonas are very similar to those of white blood cells and nuclei, it is very easy to cause interference to the detection of Trichomonas

[0006] At present, there is a fluorescent staining product on the market for fungi, anaerobic bacteria and Trichomonas in vaginal secretion samples, but this method is complicated to prepare and stain, and requires two smears and two staining on the slide, Moreover, it is necessary to switch between different microscope light sources during the observation process, which is cumbersome to operate

[0007] The traditional microbial staining technology has more interfering substances under the direct wet film microscope, and it is difficult to observe a small amount of Candida spores and trichomonas; the Gram test has poor sensitivity for the detection of trichomonas, which can easily cause missed detection

Immunofluorescence technology has also been gradually applied to the detection of vaginal microorganisms, but the detection target of this detection method is single, and multiple inspections are required for unknown samples at the same time, and the detection method is cumbersome and time-consuming.

The dry chemical enzymatic method is also less sensitive to samples with less pathogenic bacteria infection, which is not conducive to early detection, and the detection accuracy of common bloody samples, semen samples, and basal cell samples in samples is low, and it is easy to misdiagnose

PCR detection method has high sensitivity, but the operation process is complicated, time-consuming, and requires expensive instruments and reagents

Images