Reagent kit for detecting colorectal cancer on basis of liquid biopsy

Abstract

The invention provides a reagent kit for detecting colorectal cancer on the basis of liquid biopsy. The reagent kit comprises staining enhancement solution for enhancing staining effects and specific antibodies with fluorescence staining markers. The specific antibodies include CK20 antibodies, CD45 antibodies and CDX2 antibodies; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched, and whether the enriched target cells come from early-stage patients who suffer from the colorectal cancer or not can be confirmed; the tumor detection sensitivity can be improved by means of double-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, accordingly, the diversified antibodies with the fluorescence staining markers can be combined with the target cells, the target cells can be stained, the good staining effects can be realized, fluorescence is intensive, and boundaries are clear.

Description

technical field [0001] The invention relates to the field of liquid biopsy, in particular to a kit for detecting colorectal cancer based on liquid biopsy. Background technique [0002] Colorectal cancer (CRC) is a common cancer with the third highest morbidity and mortality among all cancers worldwide, with an average of 1.2 million people diagnosed with CRC each year. According to statistics, the morbidity and mortality of CRC in my country are higher than the world average. In 2011, an average of 23 patients per 100,000 people were diagnosed with CRC, and about 11 people per 100,000 people died of CRC. Moreover, in recent years, the incidence and mortality of CRC in my country have been increasing continuously. Therefore, it is particularly important for the early screening of CRC and the detection during the development of the disease. In this regard, methods based on circulating tumor cell (CTC) detection have many advantages that traditional detection methods do not h...

AI Technical Summary

Problems solved by technology

However, this method can only specifically identify the rare cells through a single monoclonal antibody, which is likely to cause missed detection

At the same time, due to the heterogeneity of cells, the amount of antigen expressed by each cell is different, resulting in sometimes very weak fluorescence intensity, which is difficult to distinguish under a fluorescence microscope

Images