44results about How to "Strong staining" patented technology

Compositions and methods are disclosed for the therapeutic use of an atonal-associated nucleic acid or amino acid sequence. Also, an animal heterozygous for an atonal-associated gene inactivation is also disclosed having at least one atonal-associated nucleic acid sequence replaced by insertion of a heterologous nucleic acid sequence used to detect expression driven by an atonal-associated promoter sequence, wherein the inactivation of the atonal-associated nucleic acid sequence prevents expression of the atonal-associated gene.

The invention relates to a water-based double-component anti-doodling paint which is a double-component paint composed of a component A and a component B. The component A comprises the following components in percentage by weight: 15-35 wt% of water-based hydroxy polyacrylate dispersion, 15-35 wt% of organosiloxane-modified water-based hydroxy polyacrylic resin, 5-15 wt% of ultraviolet-resistant filler, 0.1-0.6 wt% of defoaming agent, 0.5-1.5 wt% of leveling agent, 0.5-2 wt% of anti-aging agent, 0.5-1 wt% of wetting and dispersing agent and the balance of water, totaling 100 wt%. The component B comprises the following components in percentage by weight: 60-80 wt% of hydrophilic modified aliphatic polyisocyanate curing agent and the balance of solvent, totaling 100 wt%. The ultraviolet-resistant filler is selected from rutile titanium white. The anti-doodling paint provided by the invention has the advantages of favorable water resistance, excellent aging resistance, high medium and pollution resistance and obvious self-cleaning action.

The invention relates to a water-based anti-doodling paint which is a double-component paint composed of a component A and a component B. The component A comprises the following components in percentage by weight: 15-35 wt% of water-based hydroxy polyacrylate dispersion, 15-35 wt% of organosiloxane-modified water-based hydroxy polyacrylic resin, 5-15 wt% of ultraviolet-resistant filler, 0.1-0.6 wt% of defoaming agent, 0.5-1.5 wt% of leveling agent, 0.5-2 wt% of anti-aging agent, 0.5-1 wt% of wetting and dispersing agent and the balance of water, totaling 100 wt%. The component B comprises the following components in percentage by weight: 60-80 wt% of hydrophilic modified aliphatic polyisocyanate curing agent and the balance of solvent, totaling 100 wt%. The ultraviolet-resistant filler is selected from nano rutile titanium white. The water-based anti-doodling paint provided by the invention has the advantages of favorable water resistance, excellent aging resistance, high medium and pollution resistance and obvious self-cleaning action.

The invention provides a liver cancer detection kit based on liquid biopsy. The liver cancer detection kit comprises staining enhancing liquid and specific antibodies with fluorescent staining labels, wherein the staining enhancing liquid is used for enhancing a staining effect; the specific antibodies include an ASGPR1 antibody, a CD45 antibody and a CPS1 antibody; and the staining enhancing liquid contains a surfactant with a concentration of 0.001mg / mL-1mg / mL. The kit provided by the invention can be used for effectively enriching target cells and determining whether the enriched target cells are derived from early-phase liver cancer patients. Meanwhile, by virtue of double-tumor marker detection, the tumor detection sensitivity is increased, and furthermore, the detection accuracy is guaranteed by virtue of CEP8 detection. Besides, by utilizing the staining enhancing liquid, the staining effect is enhanced, multiple antibodies with the fluorescent staining labels can be integrated with the target cells, and the target cells are stained, so that the staining effect is relatively good, the fluorescence is relatively strong, and borders are clear.

A reactive dye compound comprising: (a) at least one chromophore moiety; (b) at least one SO2C2H4 group which is attached to the chromophore moiety either directly via the sulphur atom of the SO2C2H4 group or a linking group L; characterised in that at least one SO2C2H4 group is substituted on its terminal carbon atom with at least one Y group wherein Y is -A(CO)R* wherein A is selected from O or S and wherein R' contains at least one terminal nucleophilic group, such as OH, NH2, SH, COOH, N, NHR<1> and NR<1>R<2> wherein R<1> and R<2> may be the same or different and may be selected from C1-C4 alkyl; and salts thereof. Also claimed is a process of manufacture of the compounds herein and products obtainable by the process. The compounds herein have high Exhaustion Values (E), high Fixation Values (F) and high Efficiency Values (T) and show significant improvements in terms of reducing spent dyestuff in effluent, increasing dye affinity to the substrate, increasing the dye-substrate covalent bonding, increasing the ability to dye substrates at room temperature, decreasing the amount of dye that is removed during the post dyeing 'soaping off process' and therefore simplifying the post dyeing 'soaping off process' traditionally associated with dyeing cotton with fibre reactive dyes and reduction of staining of adjacent white fabrics. In addition, the compounds prepared above provide more intense dyeings and require less levels of salt for dyeing cotton substrates.

The invention provides a reagent kit for detecting non-small cell lung cancer on the basis of liquid biopsy. The reagent kid comprises staining enhancement solution for enhancing staining effects and specific ligands with fluorescent staining markers. The specific ligands comprise EGFR (epidermal growth factor receptor) antibodies, CD45 antibodies and folate ligands; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched by the reagent kit, and whether the enriched target cells come from early-stage patients suffering from the non-small cell lung cancer or not can be verified; the CTC (circulating tumor cell) detection sensitivity can be improved by means of double-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, the diversified ligands with the fluorescent staining markers can be combined with the surfaces of the target cells, accordingly, the surfaces of the target cells can be stained, the good staining effects can be realized, fluorescence is intense, and boundaries are clear.

The invention discloses a preparation process and a dyeing process of nanometer liquid black. The preparation process comprises the following steps of firstly, performing oxidation modification on thesurface of carbon black by adopting a liquid-phase oxidant, and performing drying to obtain oxidized carbon black; mixing and dissolving the oxidized carbon black and a dispersing agent, and carryingout processing by an ultrasonic cell crusher to obtain a nanometer oxidized carbon black dispersion liquid; and finally, adding a modified fullerene and a silane coupling agent into the nanometer oxidized carbon black dispersion liquid, heating and performing ultrasonic dispersion, adding an emulsifier and a defoaming agent, and uniformly mixing to obtain the nanometer liquid black. In the subsequent dyeing process, the nanometer liquid black only needs to be diluted with deionized water, and then a fabric is directly heated and dyed. The modified fullerene is a hydrophilic fullerene derivative, so that the dispersion effect of the oxidized carbon black can be remarkably improved, and the subsequent dyeing effect can also be effectively improved. The prepared nanometer liquid black has the advantages of high dispersibility, strong dyeing property, good color fixing effect, simple subsequent dyeing process and the like.

The invention provides a Tujia saddlebag, belonging to the technical field of articles for daily life. The Tujia saddlebag is characterized in that mixed yarns are taken as raw materials, and a production method of the saddlebag comprises the steps of weaving the mixed yarns by a Tujia weaving method and soaking the yarn raw material in impregnating liquid with full and lasting aroma, bacterium resistance and virus resistance, so that the saddlebag not only has the national features of the Tujia, but also has original functions and new functions of aroma distributing, bacterium resistance and epidemic prevention. Therefore, the life quality and the health level of people can be improved, and the Tujia saddlebag is suitable for popularization and application on a large scale.

The invention relates to a yellow colorant for fertilizer coloration, comprising components by weight percentage of: 5-100 percent of lemon yellow, 0-75 percent of toner, 0-5 percent of antiseptic and 0-95 percent of solvent, wherein the chemical name of the lemon yellow is 1-(4-sulfophenyl)-4-(4-azosulfophenyl)-5-pyrazolone-3-carboxylic acid trisodium salt; the lemon yellow, the toner, the antiseptic and the solvent are added to a reaction kettle; and all of the components are stirred for 25-35min by steam heating for uniformly mixing and solving to obtain the yellow colorant for the fertilizer coloration. In the invention, the yellow colorant has the advantages of low cost, low using amount, no pollution, easy production and the like; simultaneously, the colored fertilizer produced by the yellow colorant is easy to popularize and use.

The invention discloses a cleaner production method of ecological tanning of Tan sheep skin, comprising the steps of preparation prior to tanning, degreasing, soaking in water, fleshing, degreasing, soaking in water, softening and pickling, tanning, neutralizing, wet grinding and oxidizing washing, fat liquoring, neutralizing, outputting skin and standing and clearing up. The softening and pickling comprises the following steps: edible salt, formic acid and water are mixed to obtain first mixed solution; fur fatting agent and softening enzyme are added into the first mixed solution and mixed uniformly to obtain softened pickling solution and the softened pickling solution is heated to 48 DEG C; a Tan sheep skin is put in the softened pickling solution in a drum, softening enzyme is added into the softened pickling solution again, the drum rotates continuously for 6 hours and the softened pickling solution is filtered. The tanning comprises the following steps: the filtered softened pickling solution is put again in the drum in which the Tan sheep skin is put, formic acid solution and sulfuric acid solution are added into the filtered softened pickling solution and the drum is rotated; edible salt, formic acid, white tanning agent and water are mixed to obtain second mixed solution and the drum is rotated continuously; sodium formate, bicarb and soda ash are added for alkali adjustment; then the tanning of the Tan sheep skin is finished.

The invention relates to a green fertilizer coloring agent and a preparation method thereof. The green fertilizer coloring agent comprises the following components in part by weight: 5 to 8 parts of coloring agent, 0 to 3 parts of toner, 0 to 10 parts of dispersant and 0 to 10 parts of compatibilizer, wherein the coloring agent is food-grade lemon yellow 1-(4-sulfophenyl)-4-(4-sulfophenyl azo)-5-pyrazolone-3-carboxylic trisodium salt and / or food-grade brilliant blue di[-N-nethyl-N-3(sulfonic benzyl) aminophenyl]-2 sulfonic methylphenyl disodium salt, and the toner is food-grade rose bengal; the green fertilizer coloring agent is prepared by adding the substances into the water according to proportion, and grinding and uniformly mixing the mixture; and the using amount of the green fertilizer coloring agent in each ton of fertilizer is 0.20 to 3.5 kilograms, and the green fertilizer coloring agent is used for coloring the fertilizer to obtain corresponding green fertilizer. The green fertilizer coloring agent has the advantages of strong coloring capability, ecological environment protection, low cost, low using amount, pollution free and the like.

The invention provides a reagent kit for detecting colorectal cancer on the basis of liquid biopsy. The reagent kit comprises staining enhancement solution for enhancing staining effects and specific antibodies with fluorescence staining markers. The specific antibodies include CK20 antibodies, CD45 antibodies and CDX2 antibodies; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched, and whether the enriched target cells come from early-stage patients who suffer from the colorectal cancer or not can be confirmed; the tumor detection sensitivity can be improved by means of double-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, accordingly, the diversified antibodies with the fluorescence staining markers can be combined with the target cells, the target cells can be stained, the good staining effects can be realized, fluorescence is intensive, and boundaries are clear.

This invention discloses a pearl compound fiber and its making method, the pearl compound fiber is produced by the mixture and co-gather of the hydrolysis pearl and polyvinylalcohols, the method as follows: first dissolving the material, filtering, and sending them into the action kettle by a certain proportion, then stirring, mixing up, and heating the spinning original liquid, then filtering and sending it into the deaeration barrel, warming the deaeration barrel, and then spinning, drawing, drying and shaping, cutting off, curling, acetaling and tying, finally obtaining the product. The effect is that the product's bleaching and dying capability, its white and bright are all improved greatly.

The invention provides a reagent kit for detecting breast cancer on the basis of liquid biopsy. The reagent kit comprises staining enhancement solution for enhancing staining effects and specific antibodies with fluorescence staining markers. The specific antibodies include ER alpha antibodies, VIM antibodies, CD45 antibodies and HER2 antibodies; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched, whether the target cells come from early-stage patients who suffer from the breast cancer or not can be confirmed, and tumor can be classified; the tumor detection sensitivity can be improved by means of three-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, accordingly, the diversified antibodies with the fluorescence staining markers can be combined with the target cells, the target cells can be stained, the good staining effects can be realized, fluorescence is intensive, and boundaries are clear.

Compositions and methods are disclosed for the therapeutic use of an atonal-associated nucleic acid or amino acid sequence. Also, an animal heterozygous for an atonal-associated gene inactivation is also disclosed having at least one atonal-associated nucleic acid sequence replaced by insertion of a heterologous nucleic acid sequence used to detect expression driven by an atonal-associated promoter sequence, wherein the inactivation of the atonal-associated nucleic acid sequence prevents expression of the atonal-associated gene.

The invention provides a kit for detecting prostatic cancer based on liquid biopsy, comprising a staining enhancement solution for enhancing staining effect and specific antibodies with a fluorescent staining marker, wherein the specific antibodies include PSA (prostate specific antigen), CD45 antibody and PSMA (prostate specific membrane antigen); the staining enhancement solution comprises a surfactant having a concentration of 0.001-1 mg / mL. The kit herein is capable of effectively enriching target cells, determining whether the enriched target cells are derived from a patient with early prostatic cancer, and typing CTCs (circulating tumor cells); in addition, double-tumor-marker detection increases detection sensitivity, and detection accuracy is guaranteed through further CEP8 detection; furthermore, the staining effect is enhanced through the staining enhancement solution, each of various antibodies with a fluorescent staining marker can bind with a target cell, the target cells can be stained more effectively, fluorescence is greater, and the boundary is vivid.

The invention relates to a preparation method of high-elasticity soft knitted fabric. The preparation method of the high-elasticity soft knitted fabric is characterized by comprising the following steps: (1) raw material selecting and blending; (2) spinning processing; (3) weaving processing; (4) dyeing and after-finishing. The preparation method selects a reasonable pre-processing method, dyestuff and a dyeing process, so that the dye-uptake is ensured and the loss of strength is reduced. Due to the processes such as soft finishing technology, sizing and preshrinking in the method, the fabricis bright, clean and soft, and stable in size. By the whole method, the cost is greatly reduced and large-scale popularization and application are facilitated. The high-elasticity soft knitted fabricproduct prepared by the method is soft and smooth in hand feeling, good in drapability, and good in hygroscopicity and air permeability, has the functions of antisepsis, far infrared resistance and ultraviolet resistance, and has significant advantages in functionality, fashionability, additional value, economic benefit and the like compared with common fabric.

The invention belongs to the technical field of auxiliary materials for metal smelting and relates to continuous casting mold flux for boron containing steel. The continuous casting mold flux for the boron containing steel is prepared from, by weight, 3-7 parts of glass powder, 40-55 parts of premelting materials, 27-35 parts of aedelforsite, 2 parts of carbon black, 4-5 parts of graphite, 5-9 parts of sodium carbonate, 2.5-5.5 parts of sodium fluoride, 2-4 parts of bauxite, 2-5 parts of caustic calcined magnesite, 5-8 parts of boron carbide and 1.2 parts of binding agent. For crack sensitivity of the boron containing steel, selection, basicity, viscosity and melting point of raw materials of the continuous casting mold flux are controlled comprehensively, so that crack sensitivity of the boron containing steel is improved effectively, and the problems of cracks at edges of the surface of the boron containing steel is solved.

The invention discloses a bath towel, the composition of the bath towel and a preparation method thereof. The corners of the first frosted layer and the second frosted layer are wound with reinforced viscose; the first frosted layer and the second frosted layer are composed of warp yarns, weft yarns, reinforcing yarns A and reinforcing yarns B, and the warp yarns and weft yarns are perpendicular to each other , the angle between the warp yarn and the reinforcing yarn A is 45°, the raw bamboo fiber has the characteristics of good air permeability, instant water absorption, strong wear resistance and good dyeability, natural antibacterial, antibacterial, mite removal, Anti-odor and anti-ultraviolet functions, moisture absorption and breathability, antibacterial and antibacterial, warm in winter and cool in summer, green and environmental protection, etc., not only has excellent warmth retention, but also soft and skin-friendly, which improves the wearing comfort of wool fabrics. The sewing method enhances the firmness of the sewing.

The invention relates to a dye liquor for printing and dyeing of cotton cloth. The dye liquor is prepared from the following components in parts by weight: 1 to 3 parts of dye, 5 to 12 parts of polyester polyol, 8 to 15 parts of sodium dodecyl sulfonate, 3 to 5 parts of antifoaming agent, and 25 to 44 parts of water. The dye liquor has the advantages that the color fastness is high, the dyeing performance is strong, and the dye liquor is environment-friendly and practical.

A reactive dye compound comprising: (a) at least one chromophore moiety, (b) at least one nitrogen-containing heterocycle, (c) a linking group to link each chromophore moiety to each nitrogen-containing heterocycle; characterised in that at least one nitrogen-containing heterocycle is substituted with at least one thio-derivative and at least one quaternized nitrogen derivative. The reactive dye compounds of the invention high Exhaustion Values, high Fixation Values, particularly on cellulosic substrates such as cotton, and show significant improvements in terms of reducing spent dyestuff in effluent, increasing dye affinity to the substrate, increasing the dye-substrate covalent bonding, increasing the ability to dye substrates at room temperature, decreasing the amount of dye that is removed during the post dyeing ''soaping off process'' and therefore simplying the post dyeing ''soaping off process'' traditionally associated with dyeing cotton with fibre reactive dyes and reduction of staining of adjacent white fabrics. In addition, the compounds prepared above provide more intense dyeings and require less levels of salt for dyeing cotton substrates.

The invention provides a reagent kit for detecting non-small cell lung cancer on the basis of liquid biopsy. The reagent kid comprises staining enhancement solution for enhancing staining effects and specific ligands with fluorescent staining markers. The specific ligands comprise EGFR (epidermal growth factor receptor) antibodies, CD45 antibodies and folate ligands; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched by the reagent kit, and whether the enriched target cells come from early-stage patients suffering from the non-small cell lung cancer or not can be verified; the CTC (circulating tumor cell) detection sensitivity can be improved by means of double-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, the diversified ligands with the fluorescent staining markers can be combined with the surfaces of the target cells, accordingly, the surfaces of the target cells can be stained, the good staining effects can be realized, fluorescence is intense, and boundaries are clear.

The invention provides a reagent kit for detecting breast cancer on the basis of liquid biopsy. The reagent kit comprises staining enhancement solution for enhancing staining effects and specific antibodies with fluorescence staining markers. The specific antibodies include ER alpha antibodies, VIM antibodies, CD45 antibodies and HER2 antibodies; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched, whether the target cells come from early-stage patients who suffer from the breast cancer or not can be confirmed, and tumor can be classified; the tumor detection sensitivity can be improved by means of three-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, accordingly, the diversified antibodies with the fluorescence staining markers can be combined with the target cells, the target cells can be stained, the good staining effects can be realized, fluorescence is intensive, and boundaries are clear.

The invention belongs to the field of monoclonal antibodies, and particularly relates to an anti-human statin alpha (Inhistatin-alpha) monoclonal antibody as well as a preparation method, an immunodetection reagent and application thereof. The monoclonal antibody comprises a VHCDR1, a VHCDR2 and a VHCDR3 of which the amino acid sequences are shown as SEQ ID NO: 1-3, and a VLCDR1, a VLCDR2 and a VLCDR3 of which the amino acid sequences are shown as SEQ ID NO: 4-6. The anti-human statin alpha monoclonal antibody provided by the invention can accurately identify Inhibin-alpha protein expressed in paraffin tissue and frozen tissue, and compared with an imported reagent R1, the monoclonal antibody has the advantages of consistent specific evaluation result, low antibody use concentration and stronger dyeing effect, and can be used for immunodetection analysis. The monoclonal antibody has important significance for realizing localization of the monoclonal antibody and reducing reagent cost.

The invention discloses an amino acid type dye used for dyeing chemical fertilizers. Its composition includes a dye, a toner and a preservative, wherein: the dye is the main component and a mixed solution containing a variety of amino acid components; the toner is One of the food color additives containing natural brown, brown, red or black pigments, or a mixture of several; based on mass ratio, dyeing agent 80~100%, toner 0~20%, dispersant 0 ~5%, preservative 0~5%. Since amino acids have multiple uses such as dyeing, improving soil conditions, reducing pesticide residues, protecting the ecological environment, and supplementing crop nutrition, and are supplemented by the functions of environmentally non-polluting, biodegradable toners, this amino acid-based dye It has the advantages of low cost, low dosage, no pollution, rich sources of raw materials and easy production. It can be used for dyeing phosphate fertilizers, compound fertilizers or artificial organic fertilizers.

Provided are a compound functional black coloring agent and a preparation method thereof. The compound functional black coloring agent is prepared from, by weight, 0.1-0.7 part of inorganic nano-materials, 6-8 parts of color black, 0.1 part of dispersing agent, 0.1-0.15 part of lubricating agent, 0.01 part of antifoaming agent, 0.1-0.2 part of emulgator and 0.2 part of coupling reagent. The preparation method comprises the following steps of 1, pre-dispersion, wherein nano particles, the dispersing agent and the lubricating agent are stirred at high speed for 1-3 minutes, the coupling reagent is added to be stirred for 1-2 minutes, and then the color black is added to be stirred for 5-8 minutes; 2, ball-milling dispersion or ultrasonic dispersion; 3, emulsification treatment, wherein the emulgator, the antifoaming agent and a proper amount of process water are added for low-speed stirring. According to the compound functional black coloring agent, the nano-material, namely, the modified color black is adopted, and the advantages of being good in dispersion performance, high in dyeing property, low in production cost, stable in property and the like are achieved. According to the preparation method, the operating process is easy and convenient.