44results about How to "Strong staining" patented technology

Compositions and methods are disclosed for the therapeutic use of an atonal-associated nucleic acid or amino acid sequence. Also, an animal heterozygous for an atonal-associated gene inactivation is also disclosed having at least one atonal-associated nucleic acid sequence replaced by insertion of a heterologous nucleic acid sequence used to detect expression driven by an atonal-associated promoter sequence, wherein the inactivation of the atonal-associated nucleic acid sequence prevents expression of the atonal-associated gene.

The invention relates to a water-based double-component anti-doodling paint which is a double-component paint composed of a component A and a component B. The component A comprises the following components in percentage by weight: 15-35 wt% of water-based hydroxy polyacrylate dispersion, 15-35 wt% of organosiloxane-modified water-based hydroxy polyacrylic resin, 5-15 wt% of ultraviolet-resistant filler, 0.1-0.6 wt% of defoaming agent, 0.5-1.5 wt% of leveling agent, 0.5-2 wt% of anti-aging agent, 0.5-1 wt% of wetting and dispersing agent and the balance of water, totaling 100 wt%. The component B comprises the following components in percentage by weight: 60-80 wt% of hydrophilic modified aliphatic polyisocyanate curing agent and the balance of solvent, totaling 100 wt%. The ultraviolet-resistant filler is selected from rutile titanium white. The anti-doodling paint provided by the invention has the advantages of favorable water resistance, excellent aging resistance, high medium and pollution resistance and obvious self-cleaning action.

The invention relates to a water-based anti-doodling paint which is a double-component paint composed of a component A and a component B. The component A comprises the following components in percentage by weight: 15-35 wt% of water-based hydroxy polyacrylate dispersion, 15-35 wt% of organosiloxane-modified water-based hydroxy polyacrylic resin, 5-15 wt% of ultraviolet-resistant filler, 0.1-0.6 wt% of defoaming agent, 0.5-1.5 wt% of leveling agent, 0.5-2 wt% of anti-aging agent, 0.5-1 wt% of wetting and dispersing agent and the balance of water, totaling 100 wt%. The component B comprises the following components in percentage by weight: 60-80 wt% of hydrophilic modified aliphatic polyisocyanate curing agent and the balance of solvent, totaling 100 wt%. The ultraviolet-resistant filler is selected from nano rutile titanium white. The water-based anti-doodling paint provided by the invention has the advantages of favorable water resistance, excellent aging resistance, high medium and pollution resistance and obvious self-cleaning action.

A reactive dye compound comprising: (a) at least one chromophore moiety; (b) at least one SO2C2H4 group which is attached to the chromophore moiety either directly via the sulphur atom of the SO2C2H4 group or a linking group L; characterised in that at least one SO2C2H4 group is substituted on its terminal carbon atom with at least one Y group wherein Y is -A(CO)R* wherein A is selected from O or S and wherein R' contains at least one terminal nucleophilic group, such as OH, NH2, SH, COOH, N, NHR<1> and NR<1>R<2> wherein R<1> and R<2> may be the same or different and may be selected from C1-C4 alkyl; and salts thereof. Also claimed is a process of manufacture of the compounds herein and products obtainable by the process. The compounds herein have high Exhaustion Values (E), high Fixation Values (F) and high Efficiency Values (T) and show significant improvements in terms of reducing spent dyestuff in effluent, increasing dye affinity to the substrate, increasing the dye-substrate covalent bonding, increasing the ability to dye substrates at room temperature, decreasing the amount of dye that is removed during the post dyeing 'soaping off process' and therefore simplifying the post dyeing 'soaping off process' traditionally associated with dyeing cotton with fibre reactive dyes and reduction of staining of adjacent white fabrics. In addition, the compounds prepared above provide more intense dyeings and require less levels of salt for dyeing cotton substrates.

The invention provides a reagent kit for detecting non-small cell lung cancer on the basis of liquid biopsy. The reagent kid comprises staining enhancement solution for enhancing staining effects and specific ligands with fluorescent staining markers. The specific ligands comprise EGFR (epidermal growth factor receptor) antibodies, CD45 antibodies and folate ligands; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg/mL. The reagent kit has the advantages that target cells can be effectively enriched by the reagent kit, and whether the enriched target cells come from early-stage patients suffering from the non-small cell lung cancer or not can be verified; the CTC (circulating tumor cell) detection sensitivity can be improved by means of double-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, the diversified ligands with the fluorescent staining markers can be combined with the surfaces of the target cells, accordingly, the surfaces of the target cells can be stained, the good staining effects can be realized, fluorescence is intense, and boundaries are clear.

The invention discloses a preparation process and a dyeing process of nanometer liquid black. The preparation process comprises the following steps of firstly, performing oxidation modification on thesurface of carbon black by adopting a liquid-phase oxidant, and performing drying to obtain oxidized carbon black; mixing and dissolving the oxidized carbon black and a dispersing agent, and carryingout processing by an ultrasonic cell crusher to obtain a nanometer oxidized carbon black dispersion liquid; and finally, adding a modified fullerene and a silane coupling agent into the nanometer oxidized carbon black dispersion liquid, heating and performing ultrasonic dispersion, adding an emulsifier and a defoaming agent, and uniformly mixing to obtain the nanometer liquid black. In the subsequent dyeing process, the nanometer liquid black only needs to be diluted with deionized water, and then a fabric is directly heated and dyed. The modified fullerene is a hydrophilic fullerene derivative, so that the dispersion effect of the oxidized carbon black can be remarkably improved, and the subsequent dyeing effect can also be effectively improved. The prepared nanometer liquid black has the advantages of high dispersibility, strong dyeing property, good color fixing effect, simple subsequent dyeing process and the like.

The invention provides a Tujia saddlebag, belonging to the technical field of articles for daily life. The Tujia saddlebag is characterized in that mixed yarns are taken as raw materials, and a production method of the saddlebag comprises the steps of weaving the mixed yarns by a Tujia weaving method and soaking the yarn raw material in impregnating liquid with full and lasting aroma, bacterium resistance and virus resistance, so that the saddlebag not only has the national features of the Tujia, but also has original functions and new functions of aroma distributing, bacterium resistance and epidemic prevention. Therefore, the life quality and the health level of people can be improved, and the Tujia saddlebag is suitable for popularization and application on a large scale.

The invention relates to a yellow colorant for fertilizer coloration, comprising components by weight percentage of: 5-100 percent of lemon yellow, 0-75 percent of toner, 0-5 percent of antiseptic and 0-95 percent of solvent, wherein the chemical name of the lemon yellow is 1-(4-sulfophenyl)-4-(4-azosulfophenyl)-5-pyrazolone-3-carboxylic acid trisodium salt; the lemon yellow, the toner, the antiseptic and the solvent are added to a reaction kettle; and all of the components are stirred for 25-35min by steam heating for uniformly mixing and solving to obtain the yellow colorant for the fertilizer coloration. In the invention, the yellow colorant has the advantages of low cost, low using amount, no pollution, easy production and the like; simultaneously, the colored fertilizer produced by the yellow colorant is easy to popularize and use.

The invention provides a reagent kit for detecting breast cancer on the basis of liquid biopsy. The reagent kit comprises staining enhancement solution for enhancing staining effects and specific antibodies with fluorescence staining markers. The specific antibodies include ER alpha antibodies, VIM antibodies, CD45 antibodies and HER2 antibodies; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg/mL. The reagent kit has the advantages that target cells can be effectively enriched, whether the target cells come from early-stage patients who suffer from the breast cancer or not can be confirmed, and tumor can be classified; the tumor detection sensitivity can be improved by means of three-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, accordingly, the diversified antibodies with the fluorescence staining markers can be combined with the target cells, the target cells can be stained, the good staining effects can be realized, fluorescence is intensive, and boundaries are clear.

Compositions and methods are disclosed for the therapeutic use of an atonal-associated nucleic acid or amino acid sequence. Also, an animal heterozygous for an atonal-associated gene inactivation is also disclosed having at least one atonal-associated nucleic acid sequence replaced by insertion of a heterologous nucleic acid sequence used to detect expression driven by an atonal-associated promoter sequence, wherein the inactivation of the atonal-associated nucleic acid sequence prevents expression of the atonal-associated gene.

The invention belongs to the technical field of auxiliary materials for metal smelting and relates to continuous casting mold flux for boron containing steel. The continuous casting mold flux for the boron containing steel is prepared from, by weight, 3-7 parts of glass powder, 40-55 parts of premelting materials, 27-35 parts of aedelforsite, 2 parts of carbon black, 4-5 parts of graphite, 5-9 parts of sodium carbonate, 2.5-5.5 parts of sodium fluoride, 2-4 parts of bauxite, 2-5 parts of caustic calcined magnesite, 5-8 parts of boron carbide and 1.2 parts of binding agent. For crack sensitivity of the boron containing steel, selection, basicity, viscosity and melting point of raw materials of the continuous casting mold flux are controlled comprehensively, so that crack sensitivity of the boron containing steel is improved effectively, and the problems of cracks at edges of the surface of the boron containing steel is solved.

A reactive dye compound comprising: (a) at least one chromophore moiety, (b) at least one nitrogen-containing heterocycle, (c) a linking group to link each chromophore moiety to each nitrogen-containing heterocycle; characterised in that at least one nitrogen-containing heterocycle is substituted with at least one thio-derivative and at least one quaternized nitrogen derivative. The reactive dye compounds of the invention high Exhaustion Values, high Fixation Values, particularly on cellulosic substrates such as cotton, and show significant improvements in terms of reducing spent dyestuff in effluent, increasing dye affinity to the substrate, increasing the dye-substrate covalent bonding, increasing the ability to dye substrates at room temperature, decreasing the amount of dye that is removed during the post dyeing ''soaping off process'' and therefore simplying the post dyeing ''soaping off process'' traditionally associated with dyeing cotton with fibre reactive dyes and reduction of staining of adjacent white fabrics. In addition, the compounds prepared above provide more intense dyeings and require less levels of salt for dyeing cotton substrates.

The invention belongs to the field of monoclonal antibodies, and particularly relates to an anti-human statin alpha (Inhistatin-alpha) monoclonal antibody as well as a preparation method, an immunodetection reagent and application thereof. The monoclonal antibody comprises a VHCDR1, a VHCDR2 and a VHCDR3 of which the amino acid sequences are shown as SEQ ID NO: 1-3, and a VLCDR1, a VLCDR2 and a VLCDR3 of which the amino acid sequences are shown as SEQ ID NO: 4-6. The anti-human statin alpha monoclonal antibody provided by the invention can accurately identify Inhibin-alpha protein expressed in paraffin tissue and frozen tissue, and compared with an imported reagent R1, the monoclonal antibody has the advantages of consistent specific evaluation result, low antibody use concentration and stronger dyeing effect, and can be used for immunodetection analysis. The monoclonal antibody has important significance for realizing localization of the monoclonal antibody and reducing reagent cost.

Provided are a compound functional black coloring agent and a preparation method thereof. The compound functional black coloring agent is prepared from, by weight, 0.1-0.7 part of inorganic nano-materials, 6-8 parts of color black, 0.1 part of dispersing agent, 0.1-0.15 part of lubricating agent, 0.01 part of antifoaming agent, 0.1-0.2 part of emulgator and 0.2 part of coupling reagent. The preparation method comprises the following steps of 1, pre-dispersion, wherein nano particles, the dispersing agent and the lubricating agent are stirred at high speed for 1-3 minutes, the coupling reagent is added to be stirred for 1-2 minutes, and then the color black is added to be stirred for 5-8 minutes; 2, ball-milling dispersion or ultrasonic dispersion; 3, emulsification treatment, wherein the emulgator, the antifoaming agent and a proper amount of process water are added for low-speed stirring. According to the compound functional black coloring agent, the nano-material, namely, the modified color black is adopted, and the advantages of being good in dispersion performance, high in dyeing property, low in production cost, stable in property and the like are achieved. According to the preparation method, the operating process is easy and convenient.