Anti-human statin alpha monoclonal antibody, preparation method thereof, immunodetection reagent and application
A monoclonal antibody and inhibin technology, applied in the direction of anti-animal/human immunoglobulin, anti-growth factor immunoglobulin, measuring devices, etc., can solve the problems of high price and high cost of differential diagnosis
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Embodiment 1
[0032] The anti-human inhibin α monoclonal antibody of this embodiment comprises a heavy chain variable region with an amino acid sequence shown in SEQ ID NO:7 and a light chain variable region with an amino acid sequence shown in SEQ ID NO:8. The heavy chain variable region comprises VHCDR1, VHCDR2 and VHCDR3 as shown in SEQ ID NO: 1-3, and the light chain variable region comprises VLCDR1, VLCDR2 and VLCDR3 as shown in SEQ ID NO: 4-6.
[0033] Two. Specific examples of the preparation method of the monoclonal antibody against human inhibin alpha
Embodiment 2
[0035] The preparation method of the anti-human inhibin alpha monoclonal antibody of the present embodiment comprises the following steps:
[0036] (1) Rabbit immunization: add 5 kinds of immunogens (immunogens I, II, III, IV, and V are MSH6, Inhibin-α, MLH1, CK19, and PMS2) and immune partners (CSF2 protein; enhance immune effect) into water-soluble In the adjuvant, the immune reagent was obtained, and the concentration of the immune partner in the immune reagent was controlled to be 50 μg / mL, and the immune dose of the five immunogens was 100 μg / monkey. Then, New Zealand white rabbits were immunized with immune reagents, and after the immune effect was evaluated to meet expectations, the rabbits were subjected to immunogen shock immunization, so as to mobilize the rapid activation of B lymphocytes targeting the target immunogen in the rabbit body.
[0037] CSF2 protein can be prepared by a method comprising the following steps:
[0038] 1) First, by consulting the Uniprot d...
Embodiment 3
[0058] The immunological detection reagent of this embodiment is an immunohistochemical detection reagent, which is the monoclonal antibody working solution of Example 1, the concentration of the monoclonal antibody is 0.1 μg / mL, and the solvent is TBS buffer solution, which contains a concentration of 10 mg / mL BSA (bovine serum albumin) and preservatives Proclin-300 and Proclin-950 were added in a ratio of 1:1000 (V / V).
[0059] 4. Specific examples of the application of monoclonal antibodies in immunohistochemistry
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